北京大学学报(医学版) ›› 2019, Vol. 51 ›› Issue (2): 315-320. doi: 10.19723/j.issn.1671-167X.2019.02.022
郑苗1,詹凌璐2,刘志强3,4,李和平3,∆(),谭建国2,∆()
Miao ZHENG1,Ling-lu ZHAN2,Zhi-qiang LIU3,4,He-ping LI3,∆(),Jian-guo TAN2,∆()
摘要:
目的: 比较以不同种类气体为工作气体的大气压放电冷等离子体处理氧化锆对氧化锆表面人牙龈成纤维细胞生物学行为的影响。方法: 将20个氧化锆试件随机分为4组:对照组(无处理),氦气大气压介质阻挡放电等离子体处理90 s组(He组),氩气大气压介质阻挡放电等离子体处理90 s组(Ar组),95%氩气+5%氧气大气压介质阻挡放电等离子体处理90 s组(Ar+O2组),每组5个。分组处理后观察氧化锆表面形貌,测定表面粗糙度及润湿性,分析表面元素组成,培养人牙龈成纤维细胞3 h测定细胞在氧化锆表面的初期黏附能力,免疫荧光染色观察细胞黏附初期细胞形态。结果: 等离子体处理后氧化锆表面形貌及粗糙度无明显改变,接触角均显著降低(P<0.05), 其中Ar组及Ar+O2组接触角与He组具有统计学意义(P<0.05)。X射线光电子能谱分析结果显示,等离子体处理后氧化锆表面C/O比分别下降至0.45(He组)、0.36(Ar组)和0.33(Ar+ O2组)。经Ar及Ar+O2混合气体等离子体处理的氧化锆表面人牙龈成纤维细胞黏附初期数量提高,差异具有统计学意义(P<0.05)。相对于对照组,不同等离子体处理后的氧化锆表面成纤维细胞黏附及增殖情况更佳,细胞在黏附初期形态铺展更佳,伸出伪足更多。结论: 不同种类大气压放电冷等离子体处理氧化锆可在不改变材料表面形貌及粗糙度的情况下通过在材料表面引入含氧官能团提高材料表面润湿性;以Ar+O2混合气体为工作气体的大气压放电冷等离子体可显著提高人牙龈成纤维细胞在氧化锆表面的早期黏附能力。
中图分类号:
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