p38丝裂原活化蛋白激酶在口腔扁平苔癣及口腔鳞状细胞癌中的表达及意义

doi:10.3969/j.issn.1671-167X.2016.02.024

摘要/Abstract

摘要：

目的：探讨口腔扁平苔癣（oral lichen planus，OLP）及口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）病变组织中p38丝裂原活化蛋白激酶（mitogen activated protein kinases，MAPK）、磷酸化p38MAPK及其下游核转录因子κB（nuclear factor kappa B，NF-κB）的表达情况，初步探讨p38MAPK通路在OLP及OSCC发病过程中的作用。方法：收集53例OLP、45例OSCC及18例正常对照口腔黏膜组织的石蜡切片，免疫组织化学染色分析法检测p38MAPK、磷酸化p38MAPK及NF-κB在3组中的表达及分布情况。同时收集11例OLP、5例OSCC及7例正常对照组织的新鲜冰冻组织，采用Western blotting法进一步比较p38MAPK、磷酸化p38MAPK在3组中的表达情况。结果：免疫组织化学染色分析显示：p38MAPK及NF-κB在细胞核及细胞质中均有表达，在OLP中主要在固有层淋巴细胞中呈高表达，而在上皮细胞表达阳性率低；磷酸化p38MAPK在OSCC中阳性表达率显著高于OLP及正常对照组。Western blotting结果显示：p38MAPK在OLP、OSCC及正常对照组中均有表达；磷酸化p38MAPK在8例（8/11）OLP、5例（5/5）OSCC和4例（4/7）正常对照组中有表达。结论：p38MAPK广泛存在于口腔黏膜的各种生理、病理过程中，它的磷酸化形式可能参与了OSCC的发生和发展，其在OLP发病和进展中的作用仍需进一步探索。

关键词: p38丝裂原活化蛋白激酶, 扁平苔癣, 口腔, 癌, 鳞状细胞, NF-&kappa, B

Abstract:

Objective：To assess the differences among the expressions of p38 mitogen activated protein kinases (MAPK), phospho-p38MAPK and nuclear factor kappa B (NF-κB) in oral lichen planus (OLP) and oral squamous cell carcinoma(OSCC).Methods:In the study, 53 cases of OLP, 45 of OSCC, and 18 controls were obtained and 4-μm-thick histological sections were prepared from formalin-fixed paraffin-embedded tissue blocks.The expressions of p38MAPK,phospho-p38MAPK and NF-κB were detected by immunohistochemistry staining. Furthermore, the expressions of p38MAPK and phospho-p38MAPK were detected using Western blotting analyses in the fresh tissues from 11 cases of OLP, 5 cases of OSCC, and 7 cases of the controls. Results:p38MAPK was over-expressed in the lamina propria, but lowly expressed in the epithelium in OLP group. Phosphop38MAPK was lower expressed in OLP group than in OSCC and control groups.NF-κB was found over-expressed in the lamina propria in OLP group.p38MAPK was found expressed in all the samples in the 3 groups. The expression of phospho-p38MAPK was observed in 8 (8/11) OLP samples, 5 (5/5) OSCC samples and 4 (4/7) controls by Western blotting, but no significant differences were found within the 3 groups. Conclusion:p38MAPK can be detected in normal oral mucosa, OLP and OSCC.phospho-p38MAPK may be related to the onset and progression of OSCC. The role of p38MAPK in OLP is yet to be revealed.

Key words: p38 mitogen-activated protein kinases, Lichen planus, oral, Carcinoma, squamous cell, NF-kappa B

中图分类号:

R739.85

胡晓晟，黄云惠，刘晓松，华红. p38丝裂原活化蛋白激酶在口腔扁平苔癣及口腔鳞状细胞癌中的表达及意义[J]. 北京大学学报(医学版), 2016, 48(2): 310-315.

HU Xiao-sheng, HUANG Yun-hui, LIU Xiao-song, HUA Hong. Expression and significance of p38 mitogen-activated protein kinase in oral lichen planus and oral squamous cell cacinoma[J]. Journal of Peking University(Health Sciences), 2016, 48(2): 310-315.

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