北京大学学报(医学版) ›› 2016, Vol. 48 ›› Issue (3): 398-402. doi: 10.3969/j.issn.1671-167X.2016.03.004

• 论著 • 上一篇    下一篇

1,3-二苯-1,3-丙二酮对可卡因致小鼠神经递质含量变化的影响

陈娟1,王一超1,崔蓉1△,刘晓晓2,张宝旭2   

  1. (北京大学公共卫生学院 1. 劳动卫生与环境卫生学系,2. 毒理学系,北京100191)
  • 出版日期:2016-06-18 发布日期:2016-06-18
  • 通讯作者: 崔蓉 E-mail:cuirong19@sohu.com

Effects of 1,3-diphenyl-1,3-propanedione on neurotransmitter contents of brain in mice administered with cocaine

CHEN Juan1, WANG Yi-chao1, CUI Rong1△, LIU Xiao-xiao2, ZHANG Bao-xu2   

  1. (1. Department of Occupational and Environmental Health Sciences, 2. Department of Toxicology, Peking University School of Public Health, Beijing 100191, China)
  • Online:2016-06-18 Published:2016-06-18
  • Contact: CUI Rong E-mail:cuirong19@sohu.com

摘要:

目的:探讨1,3-二苯-1,3-丙二酮(1,3-diphenyl-1,3-propanedione,DPPD)对可卡因所致小鼠神经递质含量变化的影响。方法: 36只雄性健康的ICR小鼠随机分为对照组、可卡因组、3个DPPD预给药组(200、400、800 mg/kg)和DPPD单独给药组(800 mg/kg)。对照组灌胃给予1%(体积分数)吐温3 d;可卡因组灌胃给予1%吐温2 d,第3天皮下注射可卡因;3个DPPD预给药组小鼠分别灌胃给药(DPPD 200、400、800 mg/kg)3 d,第3天给药30 min后皮下注射可卡因;DPPD单独给药组用DPPD 800 mg/kg灌胃给药3 d。给予可卡因20 min后处死小鼠,采用高效液相色谱法荧光检测器检测小鼠脑组织中多巴胺(dopamine,DA)和5-羟色胺(5-hydroxytryptamin,5-HT)的含量,采用高效液相色谱法紫外检测器检测小鼠脑组织中谷氨酸(glutamic acid,Glu)和γ氨基丁酸(γ-aminobutyric acid,GABA)的含量,比较各组间神经递质的水平。结果: 与对照组相比,可卡因组小鼠脑中DA和GABA含量明显升高(P<0.01和P<0.05),Glu含量显著降低(P<0.05)。与可卡因组相比,DPPD预给药组(200、400、800 mg/kg)小鼠脑中DA含量明显下降(P<0.01),DPPD预给药200 mg/kg组小鼠脑中GABA含量明显下降(P<0.05)。DPPD预给药200 mg/kg组小鼠脑中DA、5-HT、Glu和GABA含量与对照组的差异均无统计学意义。结论: 低剂量DPPD可逆转可卡因致小鼠神经递质含量的改变。

关键词:  1,3-二苯-1,3-丙二酮;可卡因;神经递质;小鼠;色谱法, 高压液相

Abstract:

Objective:To investigate the possible reversal effects of 1,3-diphenyl-1,3-propanedione (DPPD) for cocaine-induced content changes of neurotransmitters of brain in mice. Methods: In this study, 36 healthy ICR male mice were randomly divided into control group, cocaine group, three DPPD pretreatment groups (200, 400, and 800 mg/kg) and DPPD alone group (800 mg/kg). The mice in control group were administered intragastrically with 1% Tween 80 for 3 d, and the mice in cocaine group were administered intragastrically with 1% Tween 80 for 2 d before cocaine was injected subcutaneously on the 3rd day. The mice in the three DPPD pretreatment groups were administered intragastrically (DPPD 200, 400, and 800 mg/kg) for 3 d before cocaine was injected subcutaneously 30 min after the administration on the 3rd day. The mice in DPPD alone group were administered intragastrically with DPPD at dose of 800 mg/kg for 3 d. The mice were sacrificed 20 minutes after cocaine injection. The contents of dopamine (DA) and 5-hydroxytryptamin (5-HT) in the mice brain were determined by high performance liquid chromatography (HPLC)-fluorescence detector, the contents of glutamic acid (Glu) and γ-aminobutyric acid (GABA) in the mice brain were determined by HPLC-ultraviolet detector, and the neurotransmitter levels were compared between the groups.  Results: The results showed that as compared with the control group, DA and GABA contents in cocaine group increased significantly (P<0.01 and P<0.05), while Glu content decreased (P<0.05). As compared with cocaine group, the DA levels in the three DPPD pretreatment groups (200, 400, and 800 mg/kg) all decreased significantly (P<0.01). In DPPD 200 mg/kg pre-administration group, GABA content decreased (P<0.05), and the contents of the four kinds of neurotransmitters had no statistical differences with those of the control group. Conclusion: DPPD may have potential reversal effects of the content changes of neurotransmitters in mice brain induced by cocaine at a lower dose.

Key words: 1,3-Diphenyl-1,3-propanedione, Cocaine, Neurotransmitter, Mice, Chromatography, high pressure liquid

中图分类号: 

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