裸小鼠淋巴结的解剖和组织学特点

doi:10.3969/j.issn.1671-167X.2017.05.027

摘要/Abstract

摘要： 目的研究裸小鼠淋巴结的解剖学和组织学特点。方法使用手术显微镜解剖20只BALB/c裸小鼠和20只BALB/c小鼠,通过免疫组织化学方法比较了两者淋巴结中T淋巴细胞和B淋巴细胞表达的不同。结果平均每只裸小鼠中包含23个淋巴结和一组大型淋巴结群。裸小鼠颈部淋巴结分布密度较高,其中颌下淋巴结及颈浅淋巴结在裸小鼠中大多数为2个(构成比分别为95%和90%),而在小鼠中大多数为4个(构成比分别为95%和90%),两者差异有统计学意义。无论小鼠还是裸小鼠的颈深淋巴结均为2个(构成比分别为100%和95%),两者差异无统计学意义。裸小鼠淋巴结中CD₃阳性的T淋巴细胞减少。细胞膜和细胞质着色的CD₃阳性表达的T细胞在裸小鼠的表达阳性率为15%,在小鼠的表达阳性率为100%,两者表达的差异有统计学意义。细胞膜阳性表达CD₂₀的B细胞在裸小鼠中的阳性率为95%,在小鼠中的阳性率为100%,两者表达的差异无统计学意义。结论裸小鼠淋巴结分布的解剖图对于初学者有一定的帮助,裸小鼠颈部淋巴结密度较高的解剖学特点和缺乏CD₃阳性的T淋巴细胞将有助于更好地理解裸小鼠的淋巴结转移机制。

关键词: 裸小鼠, 淋巴结, 解剖学

Abstract: Objective: To compare the differences of anatomical and histological characteristics of lymph nodes between BALB/c nude mice and BALB/c mice. Methods: Firstly, twenty BALB/c nude mice and twenty BALB/c mice were dissected by using a surgical microscope. Secondly, the differences of T cells and B cells at the lymph node were compared by the expressions of CD₃ and CD₂₀ immunohistochemistry dyes. Results: There were, on average, 23 nodes per mouse contained within the large lymph node assembly in the BALB/c nude mouse. The anatomical features of the lymph node distribution in the nude mice were mainly found in the neck with relatively higher density. There were two lymph nodes both in the submandible lymph nodes group and in the superficial cervical lymph nodes group (the constituent ratios were 95% and 90%, respectively) in the BALB/c nude mice, but there were four lymph nodes (the constituent ratios were 95% and 90%, respectively) in the BALB/c mice. There were significant difference between the BALB/c nude mice and the BALB/c mice. Mostly there were two lymph nodes of deep cervical lymph nodes both in the BALB/c nude mice and the BALB/c mice (the constituent ratios were 95% and 100%, respectively). There were no significant difference between the BALB/c nude mice and the BALB/c mice. We confirmed that the number of CD₃-positive T lymphocytes in lymph nodes of the nude mice decreased greatly as compared with the BALB/c mice. Expressions of CD₃ in T cells were 95% and 100% in the BALB/c nude mice and in the BALB/c mice, respectively. There were significant differences between the BALB/c nude mice and the BALB/c mice. Expressions of CD₂₀ in B cells were 95% and 100% in the BALB/c nude mice and in the BALB/c mice, respectively. There was no significant difference between the BALB/c nude mice and BALB/c mice. Conclusion: The anatomical pictures of lymph node distribution in the nude mouse will be benefit to those who are interested. The anatomical features of the lymph node local higher density in neck of the nude mouse and lack of CD₃-positive T lymphocytes would be useful for obtaining a better understanding of localized lymph node metastasis of oral transplant tumors.

Key words: Nude mice, Lymph nodes, Anatomy

中图分类号:

R-332

孙睿, 高波, 郭传瑸. 裸小鼠淋巴结的解剖和组织学特点[J]. 北京大学学报(医学版), 2017, 49(5): 893-898.

SUN Rui, GAO Bo, GUO Chuan-bin. Anatomy and histology characteristics of lymph node in nude mice[J]. Journal of Peking University(Health Sciences), 2017, 49(5): 893-898.

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