柠檬酸修饰的超顺磁性氧化铁纳米粒的制备及表征

doi:10.3969/j.issn.1671-167X.2018.02.023

北京大学学报(医学版) ›› 2018, Vol. 50 ›› Issue (2): 340-346. doi: 10.3969/j.issn.1671-167X.2018.02.023

柠檬酸修饰的超顺磁性氧化铁纳米粒的制备及表征

王欢1，覃小雅1，李子圆1，郑卓肇2，范田园1△

（1. 北京大学药学院天然及仿生药物国家重点实验室，北京大学药学院分子药剂学与新释药系统北京市重点实验室，北京100191； 2. 北京清华长庚医院核医学科，北京100044）

出版日期:2018-04-18 发布日期:2018-04-18
通讯作者: 范田园 E-mail:tianyuan_fan@bjmu.edu.cn
基金资助:
国家自然科学基金（81571779）资助

Preparation and characterization of citric acid-modified superparamagnetic iron oxide nanoparticles

WANG Huan1, QIN Xiao-ya1, LI Zi-yuan1, ZHENG Zhuo-zhao2, FAN Tian-yuan1△

(1. The State Key Laboratory of Natural and Biomimetic Drugs, Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery Systems, Peking University School of Pharmaceutical Sciences, Beijing 100191， China; 2. Department of Nuclear Medicine, Beijing Tsinghua Changgung Hospital, Beijing 100044, China)

Online:2018-04-18 Published:2018-04-18
Contact: FAN Tian-yuan E-mail:tianyuan_fan@bjmu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China (81571779)

摘要/Abstract

摘要： 目的：制备并表征柠檬酸（citric acid，CA）修饰的超顺磁性氧化铁纳米粒（superparamagnetic iron oxide nanoparticles，SPIONs）用于磁靶向、热疗和磁共振成像（magnetic resonance imaging，MRI）。方法：应用共沉淀法制备柠檬酸修饰的SPIONs（CA-SPIONs），并通过磁铁、透射电子显微镜、激光粒度测定仪、傅里叶转换红外（Fourier transform infrared，FT-IR）光谱仪、热重差热同步分析仪、振动样品磁强计、X射线衍射仪分别对CA-SPIONs的磁响应性、形态、粒径、红外特征、柠檬酸的质量分数、磁学性质、X射线衍射图谱等进行表征；采用高频感应加热机对CA-SPIONs的发热性能进行考察；通过3.0 T的MRI扫描仪对CA-SPIONs的横向弛豫效能（r2）进行评价。结果：制备的CA-SPIONs在水中分散性良好，呈深黑色，具有良好的磁响应性，其形态圆整、大小均一，平均粒径在12 nm左右，水力学平均粒径为（72.35±4.47） nm，多分散系数为0.231±0.029。红外光谱结果证明CASPIONs表面成功修饰上了CA，修饰的CA所占的重量百分比为9.0%。CA-SPIONs具有良好的超顺磁性，其饱和磁化强度为63.58 emu/g；CA-SPIONs为反尖晶石型结构，经Debye-Scherrer半宽公式可计算出晶粒大小为12.4 nm；在9 A和45~50 kHz的交变电磁场下，CA-SPIONs呈现出良好的发热性能，比吸收率（specific absorption rate，SAR）值为26 W/g；在3.0 T的MRI扫描仪下，测得CA-SPIONs的r2为338 (mmol/L)-1·s-1，表明CA-SPIONs具有良好的MRI负性对比增强作用。结论： CA-SPIONs有希望用于磁靶向、热疗和MRI诊断。

关键词: 柠檬酸, 超顺磁性氧化铁, 纳米粒, 磁共振成像, 磁靶向

Abstract: Objective： To prepare and characterize citric acid (CA)-modified super paramagnetic iron oxide nanoparticles (SPIONs) for magnetic targeting, hyperthermia and magnetic resonance imaging (MRI). Methods: CA-modified SPIONs (CA-SPIONs) were prepared by co-precipitation method and then the magnetic responsiveness, morphology, particle size, infrared feature, weight percentage of CA, magnetic property and X-ray diffraction pattern of CA-SPIONs were respectively characterized by magnet, transmission electron microscope, laser particle size analyzer, Fourier transform infrared (FT-IR) spectroscopy, thermogravimetry-differential thermal analyzer, vibrating sample magnetometer and X-ray diffractometer (XRD). The heating efficiency of the CA-SPIONs was investigated by a high frequency induction heater. The transverse relaxivity (r2) of the CA-SPIONs was evaluated by a 3.0 T MRI scanner. Results: The CA-SPIONs prepared were dispersed well in water with a dark black color and had good magnetic responsiveness. The CA-SPIONs were spherical in shape and uniform in size with an average size around 12 nm. The hydrodynamic average size of the CA-SPIONs was (72.35±4.47) nm with a polydispersity index of 0.231 ± 0.029. The result of infrared spectrum indicated that CA was successfully modified to the surface of SPIONs. The result of thermogravimetric analysis showed that the weight percentage of CA modified on the CA-SPIONs was 9.0%. The result of magnetic property evaluation demonstrated that the CA-SPIONs exhibited excellent superparamagetism with a saturation magnetism of 63.58 emu/g. The XRD result indicated that the CA-SPIONs were in inverse spinel structure. The crystallite size of the CA-SPIONs was calculated to be 12.4 nm by Debye-Scherrer equation. Under the high frequency alternating electromagnetic field with electric current of 9 A and frequency ranging from 45 to 50 kHz, the CA-SPIONs exhibited excellent heating efficiency and the specific absorption rate (SAR) value was calculated to be 26 W/g. The r2 of the CA-SPIONs was assessed to be 338 (mmol/L)-1·s-1 by a 3.0 T MRI scanner, which suggested the excellent negative contrast enhancement effect of the CA-SPIONs. Conclusion: The CA-SPIONs are expected to be used as a promising agent for magnetic targeting, hyperthermia and MRI detection.

Key words: Citric acid, Superparamagnetic iron oxide, Nanoparticles, Magnetic resonance imaging, Magnetic targeting

中图分类号:

R943

王欢，覃小雅，李子圆，郑卓肇，范田园. 柠檬酸修饰的超顺磁性氧化铁纳米粒的制备及表征[J]. 北京大学学报(医学版), 2018, 50(2): 340-346.

WANG Huan, QIN Xiao-ya, LI Zi-yuan, ZHENG Zhuo-zhao, FAN Tian-yuan. Preparation and characterization of citric acid-modified superparamagnetic iron oxide nanoparticles[J]. Journal of Peking University(Health Sciences), 2018, 50(2): 340-346.

参考文献

相关文章 15

[1]	吴一凡,张晓圆,任爽,玉应香,常翠青. 基于磁共振的青年男性股四头肌的测量和评估[J]. 北京大学学报（医学版）, 2021, 53(5): 843-849.
[2]	周迪,陈章健,胡贵平,阎腾龙,龙昌茂,冯慧敏,贾光. 纳米二氧化钛亚急性经口暴露对大鼠氧化/抗氧化生物标志和炎性因子的影响[J]. 北京大学学报（医学版）, 2020, 52(5): 821-827.
[3]	盛荟,梁磊,周童亮,贾彦兴,王彤,袁兰,韩鸿宾. 光磁双模态探针钆-[4,7-双-羧甲基-10-(2-荧光素硫脲乙基)-1,4,7,10-四氮杂环十二烷-1-基]-乙酸络合物合成方法的改进[J]. 北京大学学报（医学版）, 2020, 52(5): 959-963.
[4]	赵世明,杨铁军,许春苗,郭孝峰,马永康,陈学军,李祥,何朝宏. 3.0T磁共振成像在接受过经尿道膀胱肿瘤切除术膀胱癌中诊断肌层浸润的应用[J]. 北京大学学报（医学版）, 2020, 52(4): 701-704.
[5]	宋宇,韩鸿宾,杨军,王艾博,和清源,李媛媛,赵国梅,高亚娟,王睿,韩易兴,刘爱连,宋清伟. 脑对流增强给药对老年大鼠脑细胞外间隙微观结构的影响[J]. 北京大学学报（医学版）, 2020, 52(2): 362-367.
[6]	吴静云,米悦,刘水,姚林,唐琦,何志嵩,王霄英. MRI对肾细胞癌静脉瘤栓侵犯下腔静脉壁的术前评估[J]. 北京大学学报（医学版）, 2019, 51(4): 673-677.
[7]	许力,胡明洁,李玉玉,屈洪党,钱伟东,刘晓林. 圆锥马尾部黏液乳头型室管膜瘤继发中枢神经系统表面铁沉积症1例报道及文献复习[J]. 北京大学学报（医学版）, 2019, 51(4): 769-774.
[8]	王伟,侯进,黄文强. 运动导致兴奋脑区组织液流动一过性加速[J]. 北京大学学报（医学版）, 2019, 51(2): 206-209.
[9]	常军英,郑梅,刘颖,刘蕊,张警丰,邓晓莉. 多学科协作对神经精神狼疮磁共振成像特点的分类[J]. 北京大学学报（医学版）, 2018, 50(6): 1009-1013.
[10]	李昀倩，盛荟，梁磊，赵越，李怀业，白宁，王彤，袁兰，韩鸿宾. 光磁双模态分子探针Gd-DO3A-EA-FITC在脑组织间隙成像分析中的应用[J]. 北京大学学报(医学版), 2018, 50(2): 221-225.
[11]	赵越，李昀倩，李怀业，李玉亮，刘兰祥，袁兰，张殊佳，韩鸿宾. 荧光及磁示踪法观测脑组织液的引流分区特征[J]. 北京大学学报(医学版), 2017, 49(2): 303-309.
[12]	郑艺明，李文竹，王朝霞，张巍，吕鹤，肖江喜，袁云. 类似内收肌附丽病的抗肌萎缩蛋白病骨骼肌磁共振成像特点[J]. 北京大学学报(医学版), 2016, 48(5): 846-849.
[13]	郑艺明，杜婧，李文竹，王朝霞,张巍，肖江喜，袁云. 磁共振肌肉脂肪化色阶直方图评估法的临床应用[J]. 北京大学学报(医学版), 2016, 48(5): 830-834.
[14]	耿良，范敬，高启龙，俞静，花宝金. 人参皂苷Rg3和PEG-PLGA-Rg3纳米微粒对Lewis肺癌小鼠的作用及其机制[J]. 北京大学学报(医学版), 2016, 48(3): 496-501.
[15]	张晶,左盼莉，程克斌，于爱红，程晓光. 动态增强磁共振成像用于肌骨系统肿瘤定性诊断的可行性[J]. 北京大学学报(医学版), 2016, 48(2): 287-291.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

柠檬酸修饰的超顺磁性氧化铁纳米粒的制备及表征

Preparation and characterization of citric acid-modified superparamagnetic iron oxide nanoparticles

RICH HTML

PDF (PC)

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10