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Journal of Peking University (Health Sciences) ›› 2023, Vol. 55 ›› Issue (3): 436-441. doi: 10.19723/j.issn.1671-167X.2023.03.008

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Influence of rs2587552 polymorphism of DRD2 gene on the effect of a childhood obesity intervention: A prospective, parallel-group controlled trial

Jing CHEN1,Wu-cai XIAO1,Rui SHAN1,Jie-yun SONG2,Zheng LIU1,*()   

  1. 1. Department of Maternal and Child Health, Peking University School of Public Health, Beijing 100191, China
    2. Institute of Child and Adolescent Health, Peking University School of Public Health, Beijing 100191, China
  • Received:2023-02-06 Online:2023-06-18 Published:2023-06-12
  • Contact: Zheng LIU E-mail:liuzheng@bjmu.edu.cn
  • Supported by:
    the National Natural Science Foundation of China(81903343);National Key R&D Program of China(2016YFC1300204)

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Abstract:

Objective: To explore the association between rs2587552 polymorphism (has a strong lin-kage disequilibrium with rs1800497 which had been found in many studies to be related to obesity, r2=0.85) of DRD2 gene and the effect of a childhood obesity intervention in Chinese population, and provide a scientific basis for future personalized childhood obesity intervention based on genetic background. Methods: From a multi-center cluster randomized controlled trial studying the effect of a childhood obesity intervention, we enrolled 382 children from 8 primary schools (192 and 190 children from intervention and control groups, respectively) in Beijing as study subjects. Saliva was collected and DNA was extracted to detect the rs2587552 polymorphism of DRD2 gene, and the interactions between the gene and study arms on childhood obesity indicators [including body weight, body mass index (BMI), BMI Z-score, waist circumference, hip circumference, waist-to-hip ratio, waist-to-height ratio, and body fat percentage] were analyzed. Results: No association was found between rs2587552 polymorphism and the changes in hip circumference or body fat percentage in the intervention group (P>0.05). However, in the control group, children carrying the A allele at DRD2 rs2587552 locus showed a greater increase in hip circumference and body fat percentage compared with those not carrying A allele (P < 0.001). There were interactions between rs2587552 polymorphism of DRD2 gene and study arms on the changes in hip circumference and body fat percentage (P=0.007 and 0.015, respectively). Compared with the control group, children in the intervention group carrying the A allele at DRD2 rs2587552 locus showed decrease in hip circumference by (-1.30 cm, 95%CI: -2.25 to -0.35, P=0.007) and decrease in body fat percentage by (-1.34%, 95%CI: -2.42 to -0.27, P=0.015) compared with those not carrying A allele. The results were consistent between the dominant model and the additive model (hip circumfe-rence: -0.66 cm, 95%CI: -1.28 to -0.03, P=0.041; body fat percentage: -0.69%, 95%CI: -1.40 to 0.02, P=0.056). No interaction was found between rs2587552 polymorphism and study arms on the changes in other childhood obesity-related indicators (P>0.05). Conclusion: Children carrying the A allele at rs2587552 polymorphism of DRD2 gene are more sensitive to intervention and showed more improvement in hip circumference and body fat percentage after the intervention, suggesting that future personalized childhood obesity lifestyle intervention can be carried out based on the rs2587552 polymorphism of DRD2 gene.

Key words: Pediatric obesity, Polymorphism, single nucleotide, Life style, Intervention effect

CLC Number: 

  • R179

Table 1

Baseline characteristics of subjects"

Characteristics Intervention group (n=192) Control group (n=190) P
Age/years 9.55 ± 0.29 9.56 ± 0.31 0.596
Female 90 (46.87) 97 (51.05) 0.475
Overweight/Obesity 74 (38.54) 74 (38.95) 1.000
Obesity 48 (25.00) 41 (21.58) 0.503
Weight/kg 36.96±9.35 35.97±8.95 0.287
BMI/(kg/m2) 18.57±3.71 18.23±3.29 0.333
BMI Z-score 0.72±1.46 0.61±1.40 0.426
Waist circumference/cm 65.00±10.45 64.42±9.92 0.573
Hip circumference/cm 76.45±8.09 76.42±7.84 0.971
Waist-to-hip ratio 0.85±0.06 0.84±0.05 0.232
Waist-to-height ratio 0.46±0.07 0.46±0.06 0.735
Body fat percentage/% 21.16±10.77 20.25±9.43 0.379

Table 2

The associations between rs2587552 polymorphism and the level of obesity-related indicators at baseline"

Indicators AA/AC (n=262) GG (n=120) P
Male 134 (51.15) 61 (50.83)
Overweight/Obesity 95 (36.26) 53 (44.17) 0.136
Obesity 59 (22.52) 30 (25.00) 0.576
Age/years 9.57±0.30 9.52±0.30
Weight/kg 36.38±9.28 36.65±8.91 0.592
BMI/(kg/m2) 18.35±3.59 18.51±3.33 0.573
BMI Z-score 0.63±1.46 0.74±1.35 0.440
Waist circumference/cm 64.59±10.41 64.97±9.70 0.592
Hip circumference/cm 76.29±8.06 76.76±7.75 0.425
Waist-to-hip ratio 0.84±0.06 0.84±0.05 0.932
Waist-to-height ratio 0.46±0.06 0.46±0.06 0.604
Body fat percentage/% 20.62±10.39 20.89±9.55 0.733

Table 3

The interactions between rs2587552 polymorphism and study arms on the changes in obesity-related indicators after intervention"

Obesity-related indicatorsSNP’s effects in intervention group SNP’s effects in control group Interaction (intervention-control)
AA/AG (n=125) GG(n=67) Pa AA/AG (n=137) GG(n=53) Pa β coefficient (95%CI)b Pb
ΔWeight/kg 2.47±2.03 2.26±2.75 0.372 3.73±3.39 2.63±2.24 0.029 -0.738 (-1.880 to 0.404) 0.205
ΔBMI/(kg/m2) -0.05±0.84 -0.15±1.20 0.507 0.50±1.30 -0.01±0.90 0.006 -0.430 (-0.910 to 0.049) 0.078
ΔBMI Z-score -0.22±0.34 -0.30±0.52 0.268 0.005±0.64 -0.21±0.36 0.008 -0.162 (-0.372 to 0.049) 0.132
ΔWaist circumference/cm 1.20±3.30 0.72±2.94 0.289 1.65±2.84 0.62± 3.29 0.014 -0.702 (-2.039 to 0.635) 0.302
ΔHip circumference/cm 2.33±2.20 2.21±2.00 0.734 3.01±2.12 1.62±2.61 < 0.001 -1.302 (-2.251 to -0.353) 0.007
ΔWaist-to-hip ratio -0.01±0.04 -0.01±0.04 0.471 -0.01±0.03 -0.01±0.03 0.826 0.003 (-0.011 to 0.017) 0.671
ΔWaist-to-height ratio -0.01±0.02 -0.01±0.02 0.348 -0.004±0.02 -0.01±0.02 0.011 -0.006 (-0.015 to 0.004) 0.245
ΔBody fat percentage/% -1.03±2.79 -1.10±2.01 0.894 0.46±2.30 -0.85±2.99 < 0.001 -1.342 (-2.419 to -0.266) 0.015

Figure 1

The changes in hip circumference (A) and body fat percentage (B) of children with different genotypes of rs2587552 in intervention and control groups Data are expressed as $\bar x \pm s$. P values were tested for the interactions between rs2587552 genotypes and study arms on the changes in hip circumference or body fat percentage."

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