核转出蛋白对前列腺癌雄激素受体稳定性的调节 |
巩艳青 , 张崔建, 何世明, 李学松, 周利群, 郭应禄 |
Nuclear export signal of androgen receptor regulated of androgen receptor stability in prostate cancer |
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GONG Yan-qing
, ZHANG Cui-jian, HE Shi-ming, LI Xue-song, ZhOU Li-qun, GUO Ying-lu |
| NESAR泛素化位点的鉴定 A, ubiquitination of GFP-NESAR K776R, GFP-NESAR K807R and GFP-NESAR K776R/K807R. PC3 cells were co-transfected with expression vectors of His tagged ubiquitin with GFP or GFP-NESAR, GFP-NESAR K776R, GFP-NESAR K807R, GFP-NESAR K776R/K807R. Transfected PC3 cells were incubated overnight and then treated with vehicle or 10 μmol/L MG132 for 20 h. Cell lysates were immunoprecipitated (IP) with anti GFP-tag agarose. Protein expression was examined by Western blot (immunoblot, IB) with anti-ubiquitin and anti-GFP antibodies. Ub, ubiquitin; HC, heavy chain, 55 000; LC, light chain, 25 000. B, protein stability of GFP-NESAR K776R, GFP-NESAR K807R and GFP-NESAR K776R/K807R in the presence of CHX. PC3 cells were transfected with GFP-NESAR, GFP-NESAR K776R, GFP-NESAR K807R and GFP-NESAR K776R/K807Rrespectively. 20 h later, cells were treated with 50 mg/L CHX for indicated time points. Protein expression was examined by Western blot with anti-GFP antibody. K, lysine; R, arginine. |
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