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Journal of Peking University(Health Sciences) ›› 2019, Vol. 51 ›› Issue (6): 1036-1041. doi: 10.19723/j.issn.1671-167X.2019.06.010

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Effect of methyl eugenol on nasal mucosal aquaporin 5 in rats with allergic rhinitis

Nan WU1,Xiu-li ZHANG1,Yun HOU2,Li-xing LIN3,△(),Xiao-bing ZHANG2,△()   

  1. 1. The First Clinical Medical College of Lanzhou University, Lanzhou 730000, China
    2. Department of Otolaryngology, The First Affiliated of Lanzhou University, Lanzhou 730000, China
    3. Pediatrics, The First Affiliated of Lanzhou University, Lanzhou 730000, China
  • Received:2017-12-14 Online:2019-12-18 Published:2019-12-19
  • Contact: Li-xing LIN,Xiao-bing ZHANG E-mail:lxlin.lzu@163.com;790736924@qq.com
  • Supported by:
    Supported by the National Natural Science Foundation of China(81450052)

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Abstract:

Objective: To observe the effect of methyl eugenol on the expression of aquaporin (AQP) 5 in nasal mucosa of rats with allergic rhinitis and to explore its significance.Methods: In the study, 128 Wistar rats were randomly divided into normal control group, AR model control group, budesonide positive control group, 80 mg/kg group, 40 mg/kg group, 20 mg/kg group and 10 mg/kg group, and ovalbumin (OVA) was used to establish the model of allergic rhinitis. After successful modeling, castor oil, budesonide and corresponding doses of methyl eugenol were given respectively. After 1, 2 and 4 weeks of administration, the distribution of AQP5 in nasal mucosa was observed by immunohistochemistry. The expression of AQP5 in nasal mucosa of each group was compared by Western blotting. The expression of AQP5 mRNA was compared with real-time PCR.Results: AQP5 was mainly located in the glandular epithelium and ductal epithelial cell membrane and cytoplasm. The expression of AQP5 and AQP5 mRNA in nasal mucosa of the rats in the model control group was lower than that in the normal control group (P<0.05). AQP5 and AQP5 mRNA in nasal mucosa of the rats in each treatment group were higher than those in the model control group in varying degrees. The expression of AQP5 in the budesonide group was not significantly different from that in the normal control group 1, 2 and 4 weeks after drug intervention(P>0.05), but there was significant difference between the budesonide group and the model control group (P<0.05). The expression of AQP5 mRNA in the budesonide group was significantly different from that in the normal control group and the model control group (P<0.05).After 2 weeks of intervention, the expression of AQP5 in each dose group of methyleugenol was not significantly different from that in the budesonide group (P>0.05). After 1 week of intervention, there was no significant difference in AQP5 mRNA between the 20 mg/kg group and the normal control group (P>0.05), but there was significant difference between the 20 mg/kg group and the model control group (P<0.05).Conclusion: Methyl eugenol may increase the degree of edema of the nasal mucosa by reducing the expression of AQP5 and reduce the secretion of glands, thus alleviating the symptoms of allergic rhinitis, sneezing and runny nose.

Key words: Methyl eugenol, Aquaporin 5, Allergic rhinitis

CLC Number: 

  • R765.21

Figure 1

The distribution of AQP5 in the nasal mucosa of rats (SP ×400) A and B, Expression of AQP5 in normal control group of rat nasal mucosa; C and D, expression of AQP5 in nasal mucosa of rats in AR model control group."

Figure 2

The expression of AQP5 in the nasal mucosa of rats C, control group; A, AR model control group; B, budesonide positive control group; 80, 80 mg/kg group; 40, 40 mg/kg group; 20, 20 mg/kg group; 10, 10 mg/kg group; W, week(s). *P<0.05, comparison with normal control group; ▲ P<0.05, comparison with AR model control group; ◆ P<0.05, comparison with budesonide positive control group."

Figure 3

The expression of AQP5 mRNA in the nasal mucosa of rats C, control group; A, AR model control group; B, budesonide positive control group; 80, 80 mg/kg group; 40, 40 mg/kg group; 20, 20 mg/kg group; 10, 10 mg/kg group; W, week(s). *P<0.05, comparison with normal control group; ▲ P<0.05, comparison with AR model control group; ◆ P<0.05, comparison with budesonide positive control group."

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