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Journal of Peking University(Health Sciences) >

2016 , Vol. 48 >Issue 1: 1 - 4

DOI: https://doi.org/10.3969/j.issn.1671-167X.2016.01.001

Article

Development of human embryonic stem cell platforms for human health-safety #br# evaluation

  • YU Guang-yan ,
  • CAO Tong ,
  • ZOU Xiao-hui ,
  • ZHANG Xue-hui ,
  • FU Xin ,
  • PENG Shuang-qing ,
  • DENG Xu-liang ,
  • LI Sheng-lin ,
  • LIU He ,
  • XIAO Ran ,
  • OUYANG Hong-wei ,
  • PENG Hui ,
  • CHEN Xiao ,
  • ZHAO Zeng-ming ,
  • WANG Xiao-ying……
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  • (1. Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, China； 2. Faculty of Dentistry Research Laboratories，National University of Singapore, Singapore 119083，Singapore; 3. Centre for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou 310058, China; 4. Department of Dental Materials, Peking University School and Hospital of Stomatology, Beijing 100081, China； 5.Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Science, Beijing 100144, China; 6. Evaluation and Research Center for Toxicology, Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China; 7. Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China； 8. Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China； 9. Third Clinical Division, Peking University School and Hospital of Stomatology, Beijing 100081, China)

Online published: 2016-02-18

Supported by

Supported by the International Scientific and Technical Cooperation Pragram of China (2011DFA32190）

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Abstract

The human embryonic stem cells (hESCs) serve as a self-renewable, genetically-healthy, pluripotent and single source of all body cells, tissues and organs. Therefore, it is considered as the good standard for all human stem cells by US, Europe and international authorities. In this study, the standard and healthy human mesenchymal progenitors, ligament tissues, cardiomyocytes, keratinocytes, primary neurons, fibroblasts, and salivary serous cells were differentiated from hESCs. The human cellular health-safety of NaF, retinoic acid, 5-fluorouracil, dexamethasone, penicillin G, adriamycin, lead acetate PbAc, bisphenol A-biglycidyl methacrylate (Bis-GMA) were evaluated selectively on the standar-dized platforms of hESCs, hESCs-derived cardiomyocytes, keratinocytes, primary neurons, and fibroblasts. The evaluations were compared with those on the currently most adopted cellular platforms. Parti-cularly, the sensitivity difference of PM2.5 toxicity on standardized and healthy hESCs derived fibroblasts, currently adopted immortalized human bronchial epithelial cells Beas-2B and human umbilical vein endothelial cells (HUVECs) were evaluated. The results showed that the standardized hESCs cellular platforms provided more sensitivity and accuracy for human cellular health-safety evaluation.

Key words： Embryonic stem cells; Toxicity tests; Cell differentiation; In vitro; Humans

Cite this article

YU Guang-yan , CAO Tong , ZOU Xiao-hui , ZHANG Xue-hui , FU Xin , PENG Shuang-qing , DENG Xu-liang , LI Sheng-lin , LIU He , XIAO Ran , OUYANG Hong-wei , PENG Hui , CHEN Xiao , ZHAO Zeng-ming , WANG Xiao-ying…… . Development of human embryonic stem cell platforms for human health-safety #br# evaluation[J]. Journal of Peking University(Health Sciences), 2016 , 48(1) : 1 -4 . DOI: 10.3969/j.issn.1671-167X.2016.01.001

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