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In vitro fertilization-embryo transfer affects focal adhension kinase signaling pathway in early placenta
Received date: 2018-04-23
Online published: 2019-02-26
Supported by
Supported by the National Natural Science Foundation of China(81070493)
Objective: To study the effects of in vitro fertilization-embryo transfer (IVF-ET) technique on gene expression of focal adhension kinase (FAK) signaling pathway in early placental trophoblast cells, and to explore the effects of IVF-ET technology on the development and function of early placenta. Methods: We collected 7-8 weeks of gestation placenta tissue as a study group by ultrasound guided reduction of fetal from double embryo transfer under IVF-ET technology. In the control group, placenta tissues were obtained from the spontaneous abortion of natural pregnancy twin 7-8 weeks. Microarray hybridization analysis was performed on the placenta tissue of the two groups using the Affymetrix HG-U133 Plus 2.0 gene chip. Eight differentially expressed genes were identified by real-time quantitative polyme-rase chain reaction (qRT-PCR), and unsupervised clustering analysis and functional bioinformatics analy-sis were performed for the differentially expressed genes. Results: Twenty-eight cases of IVF-ET reduced fetal villi and 8 cases of spontaneous abortion villi were collected. A total of 8 placental villi were detected by the gene chip. Compared with the natural pregnancy control group, 32 differentially expressed genes in the placental FAK signaling pathway were expressed in IVF-ET. The differential expression was greater than or equal to 2 times, of which 12 genes were up-regulated and 20 were down-regulated. The qRT-PCR showed that the expression of the 8 genes in FAK signaling pathways of IVF-ET was significantly different from that in the placenta of natural pregnancy, which was consistent with the result of the gene chip detection. The FAK signal pathway gene localization showed that the FAK gene was mainly located in the upstream of the signal pathway in the placenta of IVF-ET. The placental trophoblast cells maintained the FAK signaling pathway function through gene expression compensation. Conclusion: There are gene expression differences in the FAK signaling pathway between the IVF-ET derived early placenta and the natural pregnancy placenta. The differentially expressed genes are involved in many key functions of the FAK signaling pathway and affect the early development and function of the IVF-ET placenta, while the placental trophoblast cells change gene expression for interference to compensate for IVF-ET technology itself, maintain normal function of the FAK signaling pathway, and satisfy the need for placental and fetal development.
Liang ZHAO , Li-fang SUN , Xiu-li ZHENG , Jing-fang LIU , Rong ZHENG , Ying WANG , Rui YANG , Lei ZHANG , Li YU , Han ZHANG . In vitro fertilization-embryo transfer affects focal adhension kinase signaling pathway in early placenta[J]. Journal of Peking University(Health Sciences), 2019 , 51(1) : 151 -158 . DOI: 10.19723/j.issn.1671-167X.2019.01.026
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