Objective: To evaluate the changes of heart structure and function in elite Chinese weightlifters by spot tracking technique. Methods: Chinese elite male weightlifters (weightlifter group, n=16) and age-matched healthy men (control group, n=16) were included as subjects. Transthoracic echocardiography and speckle-tracking automatic functional imaging were used for two-dimensional myocardial strain measurements. Results: The thickness of septum and left ventricular (LV) posterior wall and the myocardial mass index of LV were all higher than those of the control group [(9.3±1.3) mm vs. (8.0±0.4) mm, (9.2±0.8) mm vs. (8.0±0.8) mm, (77.8±12.8) g/m² vs. (67.8±11.2) g/m², all P<0.05]. Although the LV ejection fraction (LVEF) and global long axis strain value (LVGLS) were not significantly different from those in the control group, the LV mean Sm and Em reflecting the systolic and diastolic functions of the LV were lower than those in the control group (P<0.05). Further myocardial strain analysis showed that the absolute value of the long axial strain of the basal anteroseptal and mid-inferoseptal segments of the weightlifters were significantly lower than those of the control group [ \left|(-15.1\pm 4.2)\mathrm{\%}\right| vs. \left|(-18.7\pm 3.0)\mathrm{\%}\right|, \left|(-18.8\pm 2.6)\mathrm{\%}\right| vs. \left|(-21.3\pm 2.8)\mathrm{\%}\right|, all P<0.05]. There was no significant difference in other segments. The athletes were divided into two groups according to their best performance in the National Youth Games. The athletes were divided into two sub-groups according to their performance in the National Youth Games. The thickness of the septum in the sub-group with better performance (who ranked the 1^st to 8^th) was larger [(10.2±1.1) mm vs. (8.5±1.0) mm, P<0.05], and the absolute value of the long-axis strain in the mid-inferoseptal segment was lower [ \left|(-17.1\pm 2.1)\mathrm{\%}\right| vs. \left|(-20.4\pm 2.1)\mathrm{\%}\right|, P<0.05]. Conclusion: The thickening of septum is more obvious in the excellent weightlifters, accompanied by the decrease of myocardial systolic function. The speckle-tracking technique of echocardiography can identify the changes of the heart structure and function of elite athletes at an early stage, which may provide a basis for sports medicine supervision and the selection of excellent talents.