Journal of Peking University (Health Sciences) ›› 2025, Vol. 57 ›› Issue (1): 73-77. doi: 10.19723/j.issn.1671-167X.2025.01.011

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Durability of the anti-demineralization effects of fluoride varnish on dental root surfaces: An in vitro study

Hongyan TIAN1, Xue CAI2,*(), Xiaoyan WANG2   

  1. 1. First Clinical Division, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, China
    2. Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, China
  • Received:2024-09-26 Online:2025-02-18 Published:2025-01-25
  • Contact: Xue CAI E-mail:caixue@bjmu.edu.cn
  • Supported by:
    the National Natural Science Foundation of China(51602008)

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Abstract:

Objective: To study the durability of the anti-demineralization effects of fluoride varnish after being applied to dental root surfaces. Methods: Coronal and radicular dentin samples were prepared from extracted human teeth. Duraphat® (DP) was applied to the dentine surfaces to form a protective film. The film-dentin interfaces were observed by scanning electron microscopy (SEM) and the fluoride element was analyzed with energy dispersive spectrometer (EDS). Thus, the differences between applying DP on crowns and roots were compared. Radicular dentin samples were prepared and randomly divi-ded into four groups: (1) Blank: DP was not applied, and demineralized in acetic acid (pH 4.5) for 4 days; (2) Blank+aging: DP was not applied, the samples were put into deionized water for 14 days at room temperature, and then demineralized in acetic acid (pH 4.5) for 4 days; (3) DP: DP was applied and demineralized in acetic acid (pH 4.5) for 4 days; (4) DP+aging: DP was applied, the samples were put into deionized water for 14 days at room temperature, and then demineralized in acetic acid (pH 4.5) for 4 days. Finally, SEM observation and EDS analysis of fluoride content were performed on film-dentin interfaces to evaluate the degree of demineralization, the morphology of DP film, and the penetration of fluorine. Results: The immediate penetration depth of fluoride element from DP was deeper in the coronal dentin than that in radicular dentin. The samples in the blank and blank+aging groups demine-ralized significantly after acid etching. The DP group did not undergo demineralization, and the fluorine element penetrated to (76.00±8.94) μm below the interfaces. The structure of the protective film in the DP+aging group was damaged, but the underneath dentin did not undergo demineralization. The fluorine element still remained at a depth of (5.00±3.53) μm below the interfaces. Conclusion: DP has an anti-demineralization effect on the root surface, and this effect can still be exerted for a period of time after losing the structure of protective film. It has the ability to prevent root caries and a certain durability.

Key words: Topical fluorides, Duraphat, Root caries, Durability

CLC Number: 

  • R783.1

Figure 1

Schematic diagram of coronal and radicular dentin sample processing, and the position under SEM SEM, scanning electron microscopy."

Table 1

Experimental grouping and processing methods"

BlankDPBlank+agingDP+aging
DP was appliedDP was applied
Water aging for 14 dWater aging for 14 d
Demineralized for 4 dDemineralized for 4 dDemineralized for 4 dDemineralized for 4 d

Figure 2

The representative SEM images of DP protective film on coronal and radicular dentin surfaces a1, crown blank group; b1, crown DP group; c1, root blank group; d1, root DP group; a2-d2, high magnification images of a1-d1, respectively; a3-d3, energy dispersive spectra of the white/red star area in a1-d1. White arrow, DP plug in the dentinal tubule; Hollow arrow, no plug in the dentinal tubule. D, dentin; DP, Duraphat; SEM, scanning electron microscopy; At%, atomic percent."

Figure 3

The representative SEM images of the DP protective film after aging and acid etching of root surfaces a1, blank group; b1, DP group; c1, blank+aging group; d1, DP+aging group. a2-d2, high magnification images of a1-d1, respectively; b3 and d3, energy dispersive spectra of the white/red star area in b1 and d1. White dashed line, left of which is nail polish, right of which is window area; White arrow, demineralized dentinal tubules; Black arrow, DP plug in the dentinal tubule. D, dentin; DP, Duraphat; SEM, scanning electron microscopy; At%, atomic percent."

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