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Journal of Peking University(Health Sciences) >

2019 , Vol. 51 >Issue 3: 451 - 458

DOI: https://doi.org/10.19723/j.issn.1671-167X.2019.03.012

Clinicopathological and molecular characteristics of Epstein-Barr virus associated gastric cancer: a single center large sample case investigation

  • Yang YANG ,
  • Yi-qiang LIU ,
  • Xiao-hong WANG ,
  • Ke JI ,
  • Zhong-wu LI ,
  • Jian BAI ,
  • Ai-rong YANG ,
  • Ying HU ,
  • Hai-bo HAN ,
  • Zi-yu LI ,
  • Zhao-de BU ,
  • Xiao-jiang WU ,
  • Lian-hai ZHANG ,
  • Jia-fu JI
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  • 1. Department of Gastrointestinal Cancer Center
    2. Department of Pathology
    3. Department of Biobank, Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education; Laboratory of Genetics, Peking University Cancer Hospital & Institute, Beijing 100142, China
    4. Berry Oncology Corporation, Beijing 102206, China

Received date: 2019-03-18

  Online published: 2019-06-26

Supported by

Supported by the National Natural Science Foundation of China(81502643)

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Abstract

Objective: Epstein-Barr virus associated gastric cancer (EBVaGC) is different from the traditional gastric cancer (Epstein-Barr virus non-associated gastric cancer, EBVnGC), and has unique clinicopathological features. This study investigated the largest single center cancer series so as to establish the clinicopathological and molecular characteristics of EBVaGC in China.Methods: A retrospective analysis was conducted on EBVaGC and EBVnGC patients diagnosed at Peking University Cancer Hospital from 2003 to 2018 by comparing their clinicopathological features and prognosis. The gastric cancer (GC) dataset of public database was analyzed to obtain differentially expressed genes. The expression of important genes and their association with prognosis of GC were verified in GC tissues from our hospital. Results: In this study, 3 241 GC patients were included, and a total of 163 EBVaGC (5.0%) patients were identified. Compared with EBVnGC, EBVaGC was higher in male and younger patients, and positively associated with remnant GC, poorly differentiated adenocarcinoma, and mixed type GC. EBVaGC was inversely related to lymph node metastasis. The 5-year survival rate of EBVnGC and EBVaGC was 59.6% and 63.2% respectively (P<0.05). In order to explore molecular features of EBVaGC, the Cancer Genome Atlas (TCGA) dataset was analyzed (n=240), and 7 404 significant differentially expressed genes were obtained, involving cell proliferation, apoptosis, invasion and metastasis. The down-regulated invasion/metastasis gene SALL4 and the up-regulated immune checkpoint gene PD-L1 were important molecular features of EBVaGC. Validation of these two genes in large GC series showed that the majority of the EBVaGC was SALL4 negative (1/92, 1.1%, lower than EBVnGC, 303/1 727, 17.5%), and that PD-L1 was mostly positive in EBVaGC (81/110, 73.6%, higher than EBVnGC, 649/2 350, 27.6%). GC patients with SALL4 negative and PD-L1 positive were often associated with better prognosis.Conclusion: EBVaGC is a unique subtype of GC with less metastasis and a good prognosis. It also has a distinct molecular background. The down-regulation of invasion/metastasis gene SALL4 and up-regulation of immune checkpoint gene PD-L1 are important molecular features.

Key words： Epstein-Barr virus infections; Stomach neoplasms; Clinicopathological features; Prognosis

Cite this article

Yang YANG , Yi-qiang LIU , Xiao-hong WANG , Ke JI , Zhong-wu LI , Jian BAI , Ai-rong YANG , Ying HU , Hai-bo HAN , Zi-yu LI , Zhao-de BU , Xiao-jiang WU , Lian-hai ZHANG , Jia-fu JI . Clinicopathological and molecular characteristics of Epstein-Barr virus associated gastric cancer: a single center large sample case investigation[J]. Journal of Peking University(Health Sciences), 2019 , 51(3) : 451 -458 . DOI: 10.19723/j.issn.1671-167X.2019.03.012

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