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Comparative study of differentiation potential of mesenchymal stem cells derived from orofacial system into vascular endothelial cells
Received date: 2017-10-10
Online published: 2019-10-24
Supported by
Supported by National Natural Science Youth Foundation of China(81500837)
Objective: To compare the proliferation and capacity of differentiation to vascular endothelial cells and angiogenesis induction among stem cells from human exfoliated deciduous teeth (SHED), dental pulp stem cells (DPSC) and human bone marrow mesenchymal stem cells (BMSC) from orofacial bone. Methods: SHED and DPSC were isolated from pulp tissue of the patients. BMSC were isolated from orthognathic or alveolar surgical sites. The surface markers of the cells were detected by flowcytometry. Cell counting kit-8 (CCK-8) assays were conducted to detect the proliferation ability of the cells. The cells were induced into endothelial cells with conditional medium and then the induced cells were cultured in Matrigel medium. The expression of angiogenesis-related genes such as platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31), vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor 1 (VEGFR1), vascular endothelial growth factor receptor 2 (VEGFR2) and von Willebrand Factor (vWF) were quantified by real-time PCR. The cells were cultured in chick embryo chorioallantoic membrane (CAM) and the vessels were counted after 5 days. Results: The cell surface markers CD73, CD90, CD105 and CD146 of all the stem cells were positive, CD34 and CD45 were negative. The CD146 positive rate of SHED and DPSC was higher than that of BMSC. SHED had a higher proliferation rate than DPSC and BMSC. After angiogenic induction for 14 d, 3 kinds of cells emanated pseudopodia formed grid structure long vasculature in Matrigel media. The total length of tube formation of induced BMSC (7 759.7 μm) and SHED (7 734.3 μm) was higher than DPSC (5 541.0 μm). The meshes number of induced SHED (70.7) was higher than DPSC (60) and BMSC (53.7) in Matrigel medium. The expression of CD31, VEGFR2 and vWF genes of SHED were higher than those of BMSC and DPSC. VEGFR1 gene expression of BMSC was higher than that of the other groups, and SHED was higher than DPSC. The expression of VEGF showed no difference among the cells. No deference was showed between the effect of the stem cells and negative control on new formed vessels in CAM. The total length of vessels of SHED (30.4 mm) was higher than that of the negative control (20.9 mm) and BMSC (28.0 mm). Conclusion: SHED, DPSC and BMSC can differentiate into vascular endothelial cells. SHED showed a stronger angiogenesis differentiation and proliferation potential compared with DPSC and BMSC.
Jing XIE , Yu-ming ZHAO , Nan-quan RAO , Xiao-tong WANG , Teng-jiao-zi FANG , Xiao-xia LI , Yue ZHAI , Jing-zhi LI , Li-hong GE , Yuan-yuan WANG . Comparative study of differentiation potential of mesenchymal stem cells derived from orofacial system into vascular endothelial cells[J]. Journal of Peking University(Health Sciences), 2019 , 51(5) : 900 -906 . DOI: 10.19723/j.issn.1671-167X.2019.05.018
| [1] | Sakaguchi Y, Sekiya I, Yagishita K , et al. Comparison of human stem cells derived from various mesenchymal tissues: superiority of synovium as a cell source[J]. Arthritis Rheumatol, 2005,52(8):2521. |
| [2] | Folkman J . Angiogenesis in cancer, vascular, rheumatoid and other disease[J]. Nat Med, 1995,1(1):27. |
| [3] | Carmeliet P . Angiogenesis in life, disease and medicine[J]. Nature, 2005,438(7070):932. |
| [4] | Canan A, Huseyin A, Riza KA , et al. Angiogenesis in inflammatory bowel disease[J]. In J Inflammation, 2015,2015(3):970890. |
| [5] | Tateishi-Yuyama E, Matsubara H, Murohara T . Therapeutic angiogenesis for patients with limb ischemia by autologous transplantation of bone-marrow cells: a pilot study and a randomized controlled trial[J]. Acc Current J Rev, 2002,360(9331):427. |
| [6] | Hou L, Kim JJ, Woo YJ , et al. Stem cell-based therapies to promote angiogenesis in ischemic cardiovascular disease[J]. Am J Physiol, 2016,310(4):H455. |
| [7] | Shi S, Gronthos S . Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp[J]. J Bone Miner Res, 2003,18(4):696-704. |
| [8] | Belotti A, Elli E, Speranza T , et al. Circulating endothelial cells and endothelial activation in essential thrombocythemia: results from CD146 + immunomagnetic enrichment: flow cytometry and soluble E-selectin detection [J]. Am J Hematol, 2012,87(3):319. |
| [9] | Jouve N, Despoix N, Espeli M , et al. The involvement of CD146 and its novel ligand galectin-1 in apoptotic regulation of endothelial cells[J]. J Biol Chem, 2013,288(4):2571-2579. |
| [10] | Nakamura S, Yamada Y, Katagiri W , et al. Stem cell proliferation pathways comparison between human exfoliated deciduous teeth and dental pulp stem cells by gene expression profile from promising dental pulp[J]. J Endodont, 2009,35(11):1536-1542. |
| [11] | Duttenhoefer F, Lara d FR, Meury T, et al. 3D scaffolds co-seeded with human endothelial progenitor and mesenchymal stem cells: evidence of prevascularisation within 7 days[J]. Eur Cells Mater, 2013,26(4):49. |
| [12] | Moore MC, Pandolfi V, Mcfetridge PS . Novel human-derived extracellular matrix induces in vitro, and in vivo, vascularization and inhibits fibrosis[J]. Biomaterials, 2015,49:37. |
| [13] | Nourse MB, Halpin DE, Scatena M , et al. VEGF induces differentiation of functional endothelium from human embryonic stem cells: implications for tissue engineering[J]. Arterioscler Thromb Vas Biol, 2010,30(1):80-89. |
| [14] | Oswald J, Boxberger S, J?rgensen B , et al. Mesenchymal stem cells can be differentiated into endothelial cells in vitro[J]. Stem Cells, 2004,22(3):377. |
| [15] | Sugiyama M, Iohara K, Wakita H , et al. Dental pulp-derived CD31 -/CD146 - side population stem/progenitor cells enhance recovery of focal cerebral ischemia in rats [J]. Tissue Eng Part A, 2011,17(9/10):1303-1311. |
| [16] | Park S, Sorenson CM, Sheibani N . PECAM-1 isoforms, eNOS and endoglin axis in regulation of angiogenesis[J]. Clin Sci, 2015,129(3):217. |
| [17] | Zhang Z, Neiva KG, Lingen MW , et al. VEGF-dependent tumor angiogenesis requires inverse and reciprocal regulation of VEGFR1 and VEGFR2[J]. Cell Death Differ, 2010,17(3):499. |
| [18] | Ruszkowskaciastek B, Sokup A, Socha M W , et al. A preliminary evaluation of VEGF-A, VEGFR1 and VEGFR2 in patients with well-controlled type 2 diabetes mellitus[J]. J Zhejiang Univ Sci B, 2014,15(6):575-581. |
| [19] | Rondaij MG, Bierings R, Kragt A , et al. Dynamics and plasticity of Weibel-Palade bodies in endothelial cells[J]. Arterioscler Thromb Vasc Biol, 2006,26(5):1002. |
| [20] | Kleibeuker EA, Schulkens IA, Castricum KC , et al. Examination of the role of galectins during in vivo angiogenesis using the chick chorioallantoic membrane assay[J]. Methods Mol Biol, 2015,1207:305. |
| [21] | Dehelean CA, Feflea S, Gheorgheosu D , et al. Anti-angiogenic and anti-cancer evaluation of betulin nanoemulsion in chicken chorioallantoic membrane and skin carcinoma in BALB/c mice[J]. J Biomed Nanotechnol, 2013,9(4):577-589. |
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