Journal of Peking University(Health Sciences) >
Effect of subchronic combined oral exposure of titanium dioxide nanoparticles and glucose on levels of serum folate and vitamin B12 in young SD rats
Received date: 2019-12-12
Online published: 2020-06-30
Supported by
National Key Research and Develop Program of the Ministry of Science and Technology of China(2017YFC1600204);National Natural Science Foundation of China(81703257)
Objective: To explore the effect of subchronic combined oral exposure of titanium dioxide nanoparticles and glucose on levels of serum folate and vitamin B12 in young SD rats.Methods: At first, the physical and chemical properties of titanium dioxide nanoparticles, such as particle size, shape, crystal form and agglomeration degree in solution system, were characterized in detail. Eighty 4-week-old young SD rats were randomly divided into 8 groups (10 rats in each group, half male and half female). The rats were exposed to titanium dioxide nanoparticles through intragastric administration at 0, 2, 10 and 50 mg/kg body weight with or without 1.8 g/kg glucose daily for 90 days. At last, the concentrations of serum folate and vitamin B12 were detected.Results: Titanium dioxide nanoparticles were anatase crystals, closely spherical shape, with an average particle size of (24±5) nm. In male young rats, compared with the control group, the serum folate concentration was significantly increased when exposed to titanium dioxide nanoparticles (10 mg/kg) and glucose. The difference was statistically significant (P<0.05). However, in female and male young rats, compared with glucose (1.8 g/kg) exposure group, titanium dioxide nanoparticles (50 mg/kg) and glucose significantly reduced the serum folate concentration. The difference was statistically significant (P<0.05). Through statistical analysis of factorial design and calculation of interaction, obvious antagonistic effect was observed between titanium dioxide nanoparticles and glucose on the serum folate concentration in the young female SD rats. The combined oral exposure of titanium dioxide nanoparticles and glucose had little effect on the concentration of serum vitamin B12 in the young SD rats, with no significant interaction between the two substances. It was only found that titanium dioxide nanoparticles (2 mg/kg) and glucose significantly increased the serum vitamin B12 concentration, compared with glucose (1.8 g/kg) exposure group. The difference was statistically significant (P<0.05).Conclusion: Subchronic combined oral exposure of titanium dioxide nanoparticles and glucose had an obvious antagonistic effect on serum folate concentrations in young SD rats.
Key words： Folic acid; Titanium dioxide nanoparticles; Glucose; Rats; Sprague-Dawley; Vitamin B12
Zhang-jian CHEN , Shuo HAN , Pai ZHENG , Shu-pei ZHOU , Guang JIA . Effect of subchronic combined oral exposure of titanium dioxide nanoparticles and glucose on levels of serum folate and vitamin B12 in young SD rats[J]. Journal of Peking University(Health Sciences), 2020 , 52(3) : 451 -456 . DOI: 10.19723/j.issn.1671-167X.2020.03.009
| [1] | Lim JH, Bae D, Fong A. Titanium dioxide in food products: quantitative analysis using ICP-MS and Raman spectroscopy[J]. J Agric Food Chem, 2018,66(51):13533-13540. |
| [2] | FDA. Listing of color additives exempt from certification. In Code of Federal Regulations Title 21-Food and Drugs. 21 CFR 73.575. Washington, DC: US Government Printing Office, 2002. |
| [3] | Singh T, Shukla S, Kumar P, et al. Application of nanotechnology in food science: Perception and overview[J]. Front Microbiol, 2017(8):1501. |
| [4] | Yang Y, Doudrick K, Bi XY, et al. Characterization of food-grade titanium dioxide: The presence of nanosized particles[J]. Environ Sci Technol, 2014,48(11):6391-6400. |
| [5] | Weir A, Westerhoff P, Fabricius L, et al. Titanium dioxide nanoparticles in food and personal care products[J]. Environ Sci Technol, 2012,46(4):2242-2250. |
| [6] | Winkler HC, Notter T, Meyer U, et al. Critical review of the safety assessment of titanium dioxide additives in food[J]. J Nanobiotechnology, 2018,16(1):51. |
| [7] | Ruiz PA, Moron B, Becker HM, et al. Titanium dioxide nanoparticles exacerbate DSS-induced colitis: Role of the NLRP3 inflammasome[J]. Gut, 2017,66(7):1216-1224. |
| [8] | Shrivastava R, Raza S, Yadav A, et al. Effects of sub-acute exposure to TiO2, ZnO and Al2O3 nanoparticles on oxidative stress and histological changes in mouse liver and brain[J]. Drug Chem Toxicol, 2014,37(3):336-347. |
| [9] | Rollerova E, Tulinska J, Liskova A, et al. Titanium dioxide nanoparticles: Some aspects of toxicity/focus on the development[J]. Endocr Regul, 2015,49(2):97-112. |
| [10] | Ervin RB, Kit BK, Carroll MD, et al. Consumption of added sugar among U.S. children and adolescents, 2005-2008[J]. NCHS data brief, 2012(87):1-8. |
| [11] | Chen Z, Zhou D, Wang Y, et al. Combined effect of titanium dioxide nanoparticles and glucose on the cardiovascular system in young rats after oral administration[J]. J Appl Toxicol, 2019,39(4):590-602. |
| [12] | Czajka M, Sawicki K, Sikorska K, et al. Toxicity of titanium dioxide nanoparticles in central nervous system[J]. Toxicol In Vitro, 2015,29(5):1042-1052. |
| [13] | Fan J, Ye J, Kamphorst JJ, et al. Quantitative flux analysis reveals folate-dependent NADPH production[J]. Nature, 2014,510(7504):298-302. |
| [14] | Chen Z, Wang Y, Zhuo L, et al. Interaction of titanium dioxide nanoparticles with glucose on young rats after oral administration[J]. Nanomedicine, 2015,11(7):1633-1642. |
| [15] | Wang Y, Chen ZJ, Ba T, et al. Susceptibility of young and adult rats to the oral toxicity of titanium dioxide nanoparticles[J]. Small, 2013,9(9-10):1742-1752. |
| [16] | Chen Z, Zhou D, Zhou S, et al. Gender difference in hepatic toxicity of titanium dioxide nanoparticles after subchronic oral exposure in Sprague-Dawley rats[J]. J Appl Toxicol, 2019,39(5):807-819. |
| [17] | Chen ZJ, Wang Y, Wang X, et al. Effect of titanium dioxide nanoparticles on glucose homeostasis after oral administration[J]. J Appl Toxicol, 2018,38(6):810-823. |
/
| 〈 |
|
〉 |