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Journal of Peking University (Health Sciences) ›› 2020, Vol. 52 ›› Issue (3): 451-456. doi: 10.19723/j.issn.1671-167X.2020.03.009

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Effect of subchronic combined oral exposure of titanium dioxide nanoparticles and glucose on levels of serum folate and vitamin B12 in young SD rats

Zhang-jian CHEN1,Shuo HAN1,Pai ZHENG1,Shu-pei ZHOU2,Guang JIA1,△()   

  1. 1. Department of Occupational and Enviromental Health Sciences, Peking University School of Public Health, Beijing 100191, China
    2. Department of Laboratory Animal Science, Peking University Health Science Center, Beijing 100191, China
  • Received:2019-12-12 Online:2020-06-18 Published:2020-06-30
  • Contact: Guang JIA E-mail:jiaguangjia@bjmu.edu.cn
  • Supported by:
    National Key Research and Develop Program of the Ministry of Science and Technology of China(2017YFC1600204);National Natural Science Foundation of China(81703257)

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Abstract:

Objective: To explore the effect of subchronic combined oral exposure of titanium dioxide nanoparticles and glucose on levels of serum folate and vitamin B12 in young SD rats.Methods: At first, the physical and chemical properties of titanium dioxide nanoparticles, such as particle size, shape, crystal form and agglomeration degree in solution system, were characterized in detail. Eighty 4-week-old young SD rats were randomly divided into 8 groups (10 rats in each group, half male and half female). The rats were exposed to titanium dioxide nanoparticles through intragastric administration at 0, 2, 10 and 50 mg/kg body weight with or without 1.8 g/kg glucose daily for 90 days. At last, the concentrations of serum folate and vitamin B12 were detected.Results: Titanium dioxide nanoparticles were anatase crystals, closely spherical shape, with an average particle size of (24±5) nm. In male young rats, compared with the control group, the serum folate concentration was significantly increased when exposed to titanium dioxide nanoparticles (10 mg/kg) and glucose. The difference was statistically significant (P<0.05). However, in female and male young rats, compared with glucose (1.8 g/kg) exposure group, titanium dioxide nanoparticles (50 mg/kg) and glucose significantly reduced the serum folate concentration. The difference was statistically significant (P<0.05). Through statistical analysis of factorial design and calculation of interaction, obvious antagonistic effect was observed between titanium dioxide nanoparticles and glucose on the serum folate concentration in the young female SD rats. The combined oral exposure of titanium dioxide nanoparticles and glucose had little effect on the concentration of serum vitamin B12 in the young SD rats, with no significant interaction between the two substances. It was only found that titanium dioxide nanoparticles (2 mg/kg) and glucose significantly increased the serum vitamin B12 concentration, compared with glucose (1.8 g/kg) exposure group. The difference was statistically significant (P<0.05).Conclusion: Subchronic combined oral exposure of titanium dioxide nanoparticles and glucose had an obvious antagonistic effect on serum folate concentrations in young SD rats.

Key words: Folic acid, Titanium dioxide nanoparticles, Glucose, Rats, Sprague-Dawley, Vitamin B12

CLC Number: 

  • R994.4

Table 1

Physicochemical properties of titanium dioxide nanoparticles used in the present study"

Property TiO2 NPs
Shape Spherical
Average diameter (24±5) nm
Crystal structure Anatase
Purity 99.90%
Hydrodynamic diameter in H2O 40.8 nm
Hydrodynamic diameter in glucose solution 74.5 nm
Zeta potential in H2O 11.09 mV
Zeta potential in glucose solution 4.62 mV

Figure 1

Effect of combined oral exposure of titanium dioxide nanoparticles and glucose for 90 days on body weight of SD rats (x?±s, n=5)"

Figure 2

Effect of combined oral exposure of titanium dioxide nanoparticles and glucose for 90 days on the concentrations of serum folate of SD rats (x?±s, n=5) *P<0.05, compared with the control group; # P<0.05, compared with the glucose exposure group."

Figure 3

Effect of combined oral exposure of titanium dioxide nanoparticles and glucose for 90 days on the concentrations of serum vitamin B12 of SD rats (x?±s, n=5) #P<0.05, compared with the glucose exposure group."

Figure 4

Interactions of titanium dioxide nanoparticles and glucose on the concentrations of serum folate after oral exposure for 90 days in SD rats (x?±s, n=5) In a/p, a is the coefficient of interaction term between titanium dioxide nanoparticles and glucose, and p is the statistical test parameter of interaction term. The abscissa is the dose of glucose and titanium dioxide nanoparticles, and the ordinate is the concentration of folate. The two-dimensional and three-dimensional images show the changes of folate concentrations with the different doses of glucose and titanium dioxide nanoparticles, in which diffe-rent colors represent the change of ordinate."

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