Email Alert | RSS

Journal of Peking University (Health Sciences) ›› 2020, Vol. 52 ›› Issue (3): 451-456. doi: 10.19723/j.issn.1671-167X.2020.03.009

Previous Articles     Next Articles

Effect of subchronic combined oral exposure of titanium dioxide nanoparticles and glucose on levels of serum folate and vitamin B12 in young SD rats

Zhang-jian CHEN1,Shuo HAN1,Pai ZHENG1,Shu-pei ZHOU2,Guang JIA1,△()   

  1. 1. Department of Occupational and Enviromental Health Sciences, Peking University School of Public Health, Beijing 100191, China
    2. Department of Laboratory Animal Science, Peking University Health Science Center, Beijing 100191, China
  • Received:2019-12-12 Online:2020-06-18 Published:2020-06-30
  • Contact: Guang JIA E-mail:jiaguangjia@bjmu.edu.cn
  • Supported by:
    National Key Research and Develop Program of the Ministry of Science and Technology of China(2017YFC1600204);National Natural Science Foundation of China(81703257)

RICH HTML

 4   

PDF (PC)

171

Abstract:

Objective: To explore the effect of subchronic combined oral exposure of titanium dioxide nanoparticles and glucose on levels of serum folate and vitamin B12 in young SD rats.Methods: At first, the physical and chemical properties of titanium dioxide nanoparticles, such as particle size, shape, crystal form and agglomeration degree in solution system, were characterized in detail. Eighty 4-week-old young SD rats were randomly divided into 8 groups (10 rats in each group, half male and half female). The rats were exposed to titanium dioxide nanoparticles through intragastric administration at 0, 2, 10 and 50 mg/kg body weight with or without 1.8 g/kg glucose daily for 90 days. At last, the concentrations of serum folate and vitamin B12 were detected.Results: Titanium dioxide nanoparticles were anatase crystals, closely spherical shape, with an average particle size of (24±5) nm. In male young rats, compared with the control group, the serum folate concentration was significantly increased when exposed to titanium dioxide nanoparticles (10 mg/kg) and glucose. The difference was statistically significant (P<0.05). However, in female and male young rats, compared with glucose (1.8 g/kg) exposure group, titanium dioxide nanoparticles (50 mg/kg) and glucose significantly reduced the serum folate concentration. The difference was statistically significant (P<0.05). Through statistical analysis of factorial design and calculation of interaction, obvious antagonistic effect was observed between titanium dioxide nanoparticles and glucose on the serum folate concentration in the young female SD rats. The combined oral exposure of titanium dioxide nanoparticles and glucose had little effect on the concentration of serum vitamin B12 in the young SD rats, with no significant interaction between the two substances. It was only found that titanium dioxide nanoparticles (2 mg/kg) and glucose significantly increased the serum vitamin B12 concentration, compared with glucose (1.8 g/kg) exposure group. The difference was statistically significant (P<0.05).Conclusion: Subchronic combined oral exposure of titanium dioxide nanoparticles and glucose had an obvious antagonistic effect on serum folate concentrations in young SD rats.

Key words: Folic acid, Titanium dioxide nanoparticles, Glucose, Rats, Sprague-Dawley, Vitamin B12

CLC Number: 

  • R994.4

Table 1

Physicochemical properties of titanium dioxide nanoparticles used in the present study"

Property TiO2 NPs
Shape Spherical
Average diameter (24±5) nm
Crystal structure Anatase
Purity 99.90%
Hydrodynamic diameter in H2O 40.8 nm
Hydrodynamic diameter in glucose solution 74.5 nm
Zeta potential in H2O 11.09 mV
Zeta potential in glucose solution 4.62 mV

Figure 1

Effect of combined oral exposure of titanium dioxide nanoparticles and glucose for 90 days on body weight of SD rats (x?±s, n=5)"

Figure 2

Effect of combined oral exposure of titanium dioxide nanoparticles and glucose for 90 days on the concentrations of serum folate of SD rats (x?±s, n=5) *P<0.05, compared with the control group; # P<0.05, compared with the glucose exposure group."

Figure 3

Effect of combined oral exposure of titanium dioxide nanoparticles and glucose for 90 days on the concentrations of serum vitamin B12 of SD rats (x?±s, n=5) #P<0.05, compared with the glucose exposure group."

Figure 4

Interactions of titanium dioxide nanoparticles and glucose on the concentrations of serum folate after oral exposure for 90 days in SD rats (x?±s, n=5) In a/p, a is the coefficient of interaction term between titanium dioxide nanoparticles and glucose, and p is the statistical test parameter of interaction term. The abscissa is the dose of glucose and titanium dioxide nanoparticles, and the ordinate is the concentration of folate. The two-dimensional and three-dimensional images show the changes of folate concentrations with the different doses of glucose and titanium dioxide nanoparticles, in which diffe-rent colors represent the change of ordinate."

[1] Lim JH, Bae D, Fong A. Titanium dioxide in food products: quantitative analysis using ICP-MS and Raman spectroscopy[J]. J Agric Food Chem, 2018,66(51):13533-13540.
[2] FDA. Listing of color additives exempt from certification. In Code of Federal Regulations Title 21-Food and Drugs. 21 CFR 73.575. Washington, DC: US Government Printing Office, 2002.
[3] Singh T, Shukla S, Kumar P, et al. Application of nanotechnology in food science: Perception and overview[J]. Front Microbiol, 2017(8):1501.
[4] Yang Y, Doudrick K, Bi XY, et al. Characterization of food-grade titanium dioxide: The presence of nanosized particles[J]. Environ Sci Technol, 2014,48(11):6391-6400.
[5] Weir A, Westerhoff P, Fabricius L, et al. Titanium dioxide nanoparticles in food and personal care products[J]. Environ Sci Technol, 2012,46(4):2242-2250.
pmid: 22260395
[6] Winkler HC, Notter T, Meyer U, et al. Critical review of the safety assessment of titanium dioxide additives in food[J]. J Nanobiotechnology, 2018,16(1):51.
pmid: 29859103
[7] Ruiz PA, Moron B, Becker HM, et al. Titanium dioxide nanoparticles exacerbate DSS-induced colitis: Role of the NLRP3 inflammasome[J]. Gut, 2017,66(7):1216-1224.
pmid: 26848183
[8] Shrivastava R, Raza S, Yadav A, et al. Effects of sub-acute exposure to TiO2, ZnO and Al2O3 nanoparticles on oxidative stress and histological changes in mouse liver and brain[J]. Drug Chem Toxicol, 2014,37(3):336-347.
[9] Rollerova E, Tulinska J, Liskova A, et al. Titanium dioxide nanoparticles: Some aspects of toxicity/focus on the development[J]. Endocr Regul, 2015,49(2):97-112.
doi: 10.4149/endo_2015_02_97 pmid: 25960011
[10] Ervin RB, Kit BK, Carroll MD, et al. Consumption of added sugar among U.S. children and adolescents, 2005-2008[J]. NCHS data brief, 2012(87):1-8.
[11] Chen Z, Zhou D, Wang Y, et al. Combined effect of titanium dioxide nanoparticles and glucose on the cardiovascular system in young rats after oral administration[J]. J Appl Toxicol, 2019,39(4):590-602.
doi: 10.1002/jat.3750 pmid: 30427543
[12] Czajka M, Sawicki K, Sikorska K, et al. Toxicity of titanium dioxide nanoparticles in central nervous system[J]. Toxicol In Vitro, 2015,29(5):1042-1052.
[13] Fan J, Ye J, Kamphorst JJ, et al. Quantitative flux analysis reveals folate-dependent NADPH production[J]. Nature, 2014,510(7504):298-302.
[14] Chen Z, Wang Y, Zhuo L, et al. Interaction of titanium dioxide nanoparticles with glucose on young rats after oral administration[J]. Nanomedicine, 2015,11(7):1633-1642.
[15] Wang Y, Chen ZJ, Ba T, et al. Susceptibility of young and adult rats to the oral toxicity of titanium dioxide nanoparticles[J]. Small, 2013,9(9-10):1742-1752.
pmid: 22945798
[16] Chen Z, Zhou D, Zhou S, et al. Gender difference in hepatic toxicity of titanium dioxide nanoparticles after subchronic oral exposure in Sprague-Dawley rats[J]. J Appl Toxicol, 2019,39(5):807-819.
doi: 10.1002/jat.3769 pmid: 30644115
[17] Chen ZJ, Wang Y, Wang X, et al. Effect of titanium dioxide nanoparticles on glucose homeostasis after oral administration[J]. J Appl Toxicol, 2018,38(6):810-823.
pmid: 29350773
[1] WANG Gui-hong,ZUO Ting,LI Ran,ZUO Zheng-cai. Effect of rebamipide on the acute gouty arthritis in rats induced by monosodium urate crystals [J]. Journal of Peking University (Health Sciences), 2021, 53(4): 716-720.
[2] YIN Xue-qian, ZHANG Xiao-xuan, WEN Jing, LIU Si-qi, LIU Xin-ran, ZHOU Ruo-yu, WANG Jun-bo. Effects of the composite of buckwheat-oat-pea on blood glucose in diabetic rats [J]. Journal of Peking University (Health Sciences), 2021, 53(3): 447-452.
[3] Di ZHOU,Zhang-jian CHEN,Gui-ping HU,Teng-long YAN,Chang-mao LONG,Hui-min FENG,Guang JIA. Influence of oxidative/antioxidative biomarkers and inflammatory cytokines on rats after sub-acute orally administration of titanium dioxide nanoparticles [J]. Journal of Peking University (Health Sciences), 2020, 52(5): 821-827.
[4] Lei JIN,Cheng WANG,Jie ZHANG,Wen-ying MENG,Jia-yu ZHANG,Jin-hui YU,Gui-yin LIN,Ming-kun TONG,Lei JIN. Maternal periconceptional folic acid supplementation and its effects on the prevalence of fetal neural tube defects [J]. Journal of Peking University (Health Sciences), 2020, 52(4): 719-725.
[5] Zhao-nian WANG,Wen-jing GAO,Bi-qi WANG,Wei-hua CAO,Jun LV,Can-qing YU,Zeng-chang PANG,Li-ming CONG,Hua WANG,Xian-ping WU,Yu LIU,Li-ming LI. Correlation between fasting plasma glucose, HbA1c and DNA methylation in adult twins [J]. Journal of Peking University (Health Sciences), 2020, 52(3): 425-431.
[6] Shuo HAN,Zhang-jian CHEN,Di ZHOU,Pai ZHENG,Jia-he ZHANG,Guang JIA. Effects of titanium dioxide nanoparticles on fecal metabolome in rats after oral administration for 90 days [J]. Journal of Peking University (Health Sciences), 2020, 52(3): 457-463.
[7] Cheng-cheng GUO,Xiao-yuan ZHANG,Ying-xiang YU,Lan XIE,Cui-qing CHANG. Effects of chlorogenic acid on glucose tolerance and its curve characteristics in high-fat diet-induced obesity rats [J]. Journal of Peking University (Health Sciences), 2020, 52(2): 269-274.
[8] Shan-shan BAI,Si-yi MO,Xiao-xiang XU,Yun LIU,Qiu-fei XIE,Ye CAO. Characteristics of orofacial operant test for orofacial pain sensitivity caused by occlusal interference in rats [J]. Journal of Peking University(Health Sciences), 2020, 52(1): 51-57.
[9] Yong ZHANG,Chang LIU,Bin CHEN,Fan CHEN,Jin-yu DUAN,Meng-jun ZHANG,Jian JIAO. Associations of impaired glucose metabolism with chronic peridontitis in pre-diabetes patients [J]. Journal of Peking University(Health Sciences), 2020, 52(1): 71-76.
[10] Jiao HE,Ge-heng YUAN,Jun-qing ZHANG,Xiao-hui GUO. Approach to creating early diabetic peripheral neuropathy rat model [J]. Journal of Peking University(Health Sciences), 2019, 51(6): 1150-1154.
[11] Wei WANG,Jin HOU,Wen-qiang HUANG. Temporary acceleration of interstitial fluid drainage in excited brain region induced by movement [J]. Journal of Peking University(Health Sciences), 2019, 51(2): 206-209.
[12] LI Man, LI Yuan, SUN Lin, SONG Jun-lai, LV Cong. High mobility group box 1 promotes apoptosis of astrocytes after oxygen glucose deprivation/reoxygenation by regulating the expression of Bcl-2 and Bax [J]. Journal of Peking University(Health Sciences), 2018, 50(5): 785-791.
[13] HE Hai-zhen， ZHANG Ting， ZHOU Jing， WANG Dong-ping， WANG Hao-jie， SONG Yang， ZHU Zhu， WANG Pei-yu， LIU Ai-ping. Relationship between sugary drinks and diabetes of adults in Wuhai city [J]. Journal of Peking University(Health Sciences), 2018, 50(3): 469-473.
[14] LI Xiang-you, WU Jun△, LUO Dan, CHEN Wan-xian, ZHU Ge-li, ZHANG Yan-xia, BI Zhi-min, FENG Bao-hong. Effect of high glucose-based peritoneal dialysis fluids on NLRP3-IL-1β in human peritoneal mesothelial cells#br# [J]. Journal of Peking University(Health Sciences), 2017, 49(6): 954-960.
[15] YUAN Meng-meng， WANG Meng， LIU Jun-yi， ZHANG Zhi-li. Optimization of alkaline hydrolysis based on the side chain of diethyl ester 4-amino-N5 -formyl-N8,N10 -dideazatetrahydrofolic acid [J]. Journal of Peking University(Health Sciences), 2017, 49(4): 714-718.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Author. English Title Test[J]. Journal of Peking University(Health Sciences), 2010, 42(1): 1 -10 .
[2] . [J]. Journal of Peking University(Health Sciences), 2009, 41(2): 188 -191 .
[3] . [J]. Journal of Peking University(Health Sciences), 2009, 41(3): 376 -379 .
[4] . [J]. Journal of Peking University(Health Sciences), 2009, 41(4): 459 -462 .
[5] . [J]. Journal of Peking University(Health Sciences), 2010, 42(1): 82 -84 .
[6] . [J]. Journal of Peking University(Health Sciences), 2007, 39(3): 319 -322 .
[7] . [J]. Journal of Peking University(Health Sciences), 2007, 39(3): 333 -336 .
[8] . [J]. Journal of Peking University(Health Sciences), 2007, 39(3): 337 -340 .
[9] . [J]. Journal of Peking University(Health Sciences), 2007, 39(3): 225 -328 .
[10] . [J]. Journal of Peking University(Health Sciences), 2007, 39(4): 346 -350 .
Copyright © Journal of Peking University (Health Sciences), All Rights Reserved.
Powered by Beijing Magtech Co. Ltd