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Journal of Peking University (Health Sciences) ›› 2021, Vol. 53 ›› Issue (3): 447-452. doi: 10.19723/j.issn.1671-167X.2021.03.002

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Effects of the composite of buckwheat-oat-pea on blood glucose in diabetic rats

YIN Xue-qian,ZHANG Xiao-xuan,WEN Jing,LIU Si-qi,LIU Xin-ran,ZHOU Ruo-yu,WANG Jun-boΔ()   

  1. Department of Nutrition and Food Hygiene, Peking University School of Public Health, Beijing 100191, China
  • Received:2021-01-27 Online:2021-06-18 Published:2021-06-16
  • Contact: Jun-bo WANG E-mail:bmuwjbxy@bjmu.edu.cn
  • Supported by:
    National Key Research and Development Program of China(2017YFD0401202)

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Abstract:

Objective: To study the effects of buckwheat-oat-pea (BOP) composite flour [buckwheat ∶oats ∶peas=6 ∶1 ∶1 (quality ratio)] on blood glucose in diabetic rats. Methods: In this study, 64 male Sprague-Dawley rats were divided into 8 groups by fasting blood glucose (FBG) and body weight: normal control group, model control group, metformin group, buckwheat group, oats group, BOP low-dose group (BOP-L), medium-dose group (BOP-M), and high-dose group (BOP-H). The rats in the normal control group were fed with normal diet, the rats in the model control group and metformin group were fed with a high-fat diet (HFD), and the rats in the buckwheat group, oats group, and BOP-L, BOP-M, BOP-H groups were fed with HFD containing 10% buckwheat flour, 10% oat flour, 3.3% BOP, 10% BOP, 30% BOP, respectively. The HFD in all the groups had the same percentage of energy from fat (45%). After 30 days, the rats fed with HFD received intraperitoneal injection of streptozotocin (30 mg/kg, once a week for two weeks) to establish diabetes mellitus. After the model was successful established, the rats were fed for another 28 days. During the study, the body weight, food intake/body weight (FI/BW) and water intake/body weight (WI/BW), food utilization rate, 24 h urine volume, FBG, glucose area under curve (GAUC) of oral glucose tolerance test were measured regularly. At the end of the study, the fasting serum glucose and insulin were measured, and homeostasis model assessment of insulin resistance (HOMA-IR) was calculated. Results: With the inducing of HFD and streptozotocin, compared with the normal control group, the rats in the model control group had higher FI/BW, WI/BW, 24 h urine volume, FBG, GAUC, HOMA-IR (P<0.05), and lower body weight, food utilization rate (P<0.05). Compared with the model control group, the rats in the three BOP groups all had higher body weight, food utilization rate (P<0.05), and lower WI/BW, HOMA-IR (P<0.05); the rats in the BOP-L and BOP-M groups had lower FI/BW, 24 h urine volume, FBG (P<0.05), and the rats in the BOP-M group also had lower GAUC (P<0.05). After the establishment of diabetes, there was no significant difference in blood glucose and the other indicators between the rats in the three BOP groups and the buckwheat group or the oats group (P>0.05). Conclusion: The BOP had the effects of reducing blood glucose, insulin resistance and diabetic symptoms on diabetic rats, and had the value for further development and utilization.

Key words: Diet,diabetic, Food,formulated, Blood glucose, Rats,Sprague-Dawley

CLC Number: 

  • R587.1

Figure 1

Effect of the BOP on the body weight of diabetic rats ($\bar{x} \pm s$, n=8) * P<0.05, compared with the MC group; # P<0.05, compared with the OA group. Abbreviations as in Table 1."

Table 1

Effects of the BOP on the food intake/body weight, food utilization rate, water intake/body weight and 24 h urine volume of diabetic rats"

Group Food intake/body weight/
[g/(kg·d)]($\bar{x} \pm s$, n=8)		 Food utilization rate
of 1-10 weeks/%
($\bar{x} \pm s$, n=8)		 Water intake/body weight/[mL/(kg·d)]
[P50(P25, P75), n=8]		 24 h urine
volume/mL
($\bar{x} \pm s$, n=6)
1-4 weeks 5-10 weeks 1-4 weeks 5-10 weeks
NC 55.8±3.1* 42.5±2.5* 17.3±2.6* 93.1 (91.5, 154.4) 74.2 (72.1, 125.0)* 13.0±2.7*
MC 48.9±4.6 61.4±1.7 6.5±4.3 125.0 (104.0, 132.5) 300.2 (297.8, 329.2) 71.6±8.5
MET 50.1±4.5 50.7±7.8* 10.8±3.1* 102.1 (96.5, 108.9) 253.4 (145.5, 290.5)* 36.6±18.7*
BU 52.1±3.1 51.6±2.7* 11.6±2.9* 123.7 (120.2, 130.2) 283.9 (240.6, 301.3)* 39.1±13.0*
OA 50.7±3.7 52.0±4.2* 12.4±3.9* 120.6 (107.1, 124.7) 260.1 (205.1, 262.3)* 42.5±22.2*
BOP-L 52.0±3.2 50.9±7.7* 11.6±4.0* 96.3 (84.6, 96.7) 274.5 (215.1, 287.5)* 35.3±14.3*
BOP-M 50.0±3.7 51.0±3.9* 12.6±3.2* 108.8 (105.7, 116.2) 243.5 (217.3, 270.2)* 48.5±9.2*
BOP-H 55.5±6.0 56.1±6.9 11.1±1.3* 119.6 (104.0, 133.2) 257.3 (248.9, 265.5)* 58.9±20.8

Figure 2

Effect of the BOP on the fasting blood glucose of diabetic rats ($\bar{x} \pm s$, n=8) * P<0.05, compared with the MC group. Abbreviations as in Table 1."

Figure 3

Effect of the BOP on the GAUC of diabetic rats ($\bar{x} \pm s$, n=8) * P<0.05, compared with the MC group. GAUC, glucose area under curve; Other abbreviations as in Table 1."

Table 2

Effect of the BOP on the insulin of diabetic rats ($\bar{x} \pm s$, n=8)"

Group Insulin/(mIU/L)
NC 48.2±13.2
MC 57.5±36.4
MET 33.1±23.1
BU 23.5±22.6
OA 30.8±23.2
BOP-L 24.9±24.4
BOP-M 29.6±16.5
BOP-H 28.0±22.4

Figure 4

Effect of the BOP on the HOMA-IR of diabetic rats ($\bar{x} \pm s$, n=8) * P<0.05, compared with the MC group. HOMA-IR, homeostasis model assessment of insulin resistance; Other abbreviations as in Table 1."

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