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Expression of cartilage oligomeric matrix protein in the synovial chondromatosis of the temporomandibular joint
Received date: 2020-10-12
Online published: 2021-02-07
Objective: To detect the expression of cartilage oligomeric matrix protein (COMP) in the synovial chondromatosis of the temporomandibular joint (TMJSC),and to discuss the possible interactions between COMP, transforming growth factor (TGF)-β3, TGF-β1 and bone morphogenetic protein-2 (BMP-2) in the development of this neoplastic disease.Methods: Patients in Peking University School and Hospital of Stomatology from January 2011 to February 2020 were selected, who had complete medical records, TMJSC was verified histologically after operation. The expressions of COMP, TGF-β3, TGF-β1 and BMP-2 in the TMJSC of the temporomandibular joint were detected by immunohistochemistry and quantitative real-time PCR (RT-PCR) at the protein level and mRNA level respectively, compared with the normal synovial tissue of temporomandibular joint. The histological morphology, protein expression and distribution of TMJSC tissues were observed microscopically, and the positive staining proteins were counted and scored. SPSS 22.0 statistical software was used to analyze the expression differences between the related proteins in TMJSC tissue and the normal synovial tissue of temporomandibular joint and to compare their differences. P<0.05 indicated that the difference was statistically significant.Results: Immunohistochemical results showed that the positive expression of COMP in TMJSC tissues was mostly found in synovial tissues and chondrocytes adjacent to synovial tissues, and the difference was statistically significant, compared with the normal temporomandibular joint synovial tissues. The positive expression of COMP was significantly different between recurrent TMJSC and non-recurrent ones. The positive expressions of TGF-β3, TGF-β1 and BMP-2 were higher than the normal synovial tissue, and were also mostly found in the synovial cells and adjacent chondrocytes, which was further confirmed by Western blot. According to the RT-PCR results, the expressions of COMP, TGF-β3, TGF-β1 and BMP-2 in TMJSC were higher than those in the normal synovial tissue.Conclusion: The expression of COMP in TMJSC of temporomandibular joint increased significantly, compared with the normal synovial tissue. There may be interactions between COMP and cytokines related to the proliferation and differentiation, like TGF-β3, TGF-β1 and BMP-2, which may play a potential role in the pathogenesis of TMJSC.
Wei-hua HAN , Hai-yan LUO , Chuan-bin GUO , Qi NING , Juan-hong MENG . Expression of cartilage oligomeric matrix protein in the synovial chondromatosis of the temporomandibular joint[J]. Journal of Peking University(Health Sciences), 2021 , 53(1) : 34 -39 . DOI: 10.19723/j.issn.1671-167X.2021.01.006
| [1] | Meng JH, Guo CB, Yi B, et al. Clinical and radiologic findings of synovial chondromatosis affecting the temporomandibular joint[J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2010,109(3):441-448. |
| [2] | Coles MJ, Tara HH. Synovial chondromatosis: a case study and brief review[J]. Am J Orthop (Belle Mead NJ), 1997,26(1):37-40. |
| [3] | 韩方凯, 马绪臣. 颞下颌关节滑膜软骨瘤病[J]. 现代口腔医学杂志, 2006,20(4):425-428. |
| [4] | Helmy ES, Bays RA, Sharawy MM. Synovial chondromatosis associated with experimental osteoarthritis in adult monkeys[J]. J Oral Maxillofac Surg, 1989,47(8):823-827. |
| [5] | Li YJ, Cai HX, Fang W, et al. Fibroblast growth factor 2 involved in the pathogenesis of synovial chondromatosis of temporomandibular joint[J]. J Oral Pathol Med, 2014,43(5):388-394. |
| [6] | Li Y, El Mozen LA, Cai H, et al. Transforming growth factor beta 3 involved in the pathogenesis of synovial chondromatosis of temporomandibular joint[J]. Sci Rep, 2015,5:8843. |
| [7] | Sandberg MM, Aro HT, Vuorio EI. Gene expression during bone repair[J]. Clin Orthop and Relat Res, 1993(289):292-312. |
| [8] | Nishimura K, Solchaga LA, Caplan AI, et al. Chondroprogenitor cells of synovial tissue[J]. Arthritis Rheum, 2010,42(12):2631-2637. |
| [9] | Pearson CA, Pearson D, Shibahara S, et al. Tenascin: cDNA cloning and induction by TGF-beta[J]. EMBO J, 1988,7(10):2977-2982. |
| [10] | Mackie EJ, Thesleff I, Chiquet-Ehrismann R. Tenascin is asso-ciated with chondrogenic and osteogenic differentiation in vivo and promotes chondrogenesis in vitro[J]. J Cell Biol, 1987,105(6):2569-2579. |
| [11] | Nakanishi S, Sskamoto K, Yoshitake H, et al. Bone morphoge-netic proteins are involved in the pathobiology of synovial chondromatosis[J]. Biochem Biophys Res Commun, 2009,379(4):914-919. |
| [12] | Iwata H, Ono S, Sato K, et al. Bone morphogenetic protein-induced muscle- and synovium-derived cartilage differentiation in vitro[J]. Clin Orthop Relat Res, 1993(296):295-300. |
| [13] | Wang C, Liu G, Zhang W, et al. Cartilage oligomeric matrix protein improves in vivo cartilage regeneration and compression modulus by enhancing matrix assembly and synjournal[J]. Colloids Surf B Biointerfaces, 2017,159:518-526. |
| [14] | Zaucke F, Dinser R, Maurer P, et al. Cartilage oligomeric matrix protein (COMP) and collagen Ⅸ are sensitive markers for the differentiation state of articular primary chondrocytes[J]. Biochem J, 2001,358(1):17-24. |
| [15] | 游洪波, 陈安民, 王国宾, 等. 转化生长因子-β诱导前软骨干细胞成软骨分化的研究[J]. 中华创伤杂志, 2010,26(5):453-459. |
| [16] | Li H, Haudenschild DR, Posey KL, et al. Comparative analysis with collagen type Ⅱ distinguishes cartilage oligomeric matrix protein as a primary TGFβ-responsive gene[J]. Osteoarthritis Cartilage, 2011,19(10):1246-1253. |
| [17] | Haudenschild DR, Hong E, Yik JH, et al. Enhanced activity of transforming growth factor-β1 (TGF-β1) bound to cartilage oligomeric matrix protein[J]. J Biol Chem, 2011,286(50):43250-43258. |
| [18] | Guo P, Shi Z L, Liu A, et al. Effects of cartilage oligomeric matrix protein on bone morphogenetic protein-2-induced differen-tiation of mesenchymal stem cells[J]. Orthop Surg, 2015,6(4):280-287. |
| [19] | 于萍, 步宏, 王华, 等. 免疫组化结果的图像分析与人工计数方法的对比研究[J]. 生物医学工程学杂志, 2003,20(2):288-290. |
| [20] | Ardekian L, Faquin W, Troulis MJ, et al. Synovial chondromatosis of the temporomandibular joint: report and analysis of eleven cases[J]. J Oral Maxillofac Surg, 2005,63(7):941-947. |
| [21] | Fujita S, Iizuka T, Tuboi Y, et al. Synovial chondromatosis of the temporomandibular joint with immunohistochemical findings: Report of a case[J]. J Oral Maxillofac Surg, 1991,49(8):880-883. |
| [22] | Hedbom E, Antonsson P, Hjerpe A, et al. Cartilage matrix proteins: An acidic oligomeric protein (COMP) detected only in cartilage[J]. J Biol Chem, 1992,267(9):6132-6136. |
| [23] | Recklies AD, Baillargeon L, White C. Regulation of cartilage oligomeric matrix protein synjournal in human synovial cells and arti-cular chondrocytes[J]. Arthritis Rheum, 1998,41(6):997-1006. |
| [24] | Andersson ML, Thorstensson CA, Roos EM, et al. Serum levels of cartilage oligomeric matrix protein (COMP) increase temporarily after physical exercise in patients with knee osteoarthritis[J]. BMC Musculoskelet Disord, 2006,7:98. |
| [25] | Chen HC, Shah SH, Li YJ, et al. Inverse association of general joint hypermobility with hand and knee osteoarthritis and serum cartilage oligomeric matrix protein levels[J]. Arthritis Rheum, 2008,58(12):3854-3864. |
| [26] | Blumbach K, Bastiaansen-Jenniskens YM, DeGroot J, et al. Combined role of type Ⅸ collagen and cartilage oligomeric matrix protein in cartilage matrix assembly: Cartilage oligomeric matrix protein counteracts type Ⅸ collagen-induced limitation of cartilage collagen fibril growth in mouse chondrocyte cultures[J]. Arthritis Rheum, 2009,60(12):3676-3685. |
| [27] | Nozawa-Inoue K, Amizuka N, Ikeda N, et al. Synovial membrane in the temporomandibular joint: Its morphology, function and development[J]. Arch Histol Cytol, 2003,66(4):289-306. |
| [28] | 路红艳, 李健, 龙星. 间充质干细胞表面标记在颞下颌关节疾病患者滑膜间充质细胞表达的比较[J]. 口腔医学研究, 2018,34(11):1249-1252. |
| [29] | 符培亮, 丛锐军, 张雷, 等. 体外条件下TGF-β3、BMP-2和DEX诱导兔滑膜间充质干细胞向软骨细胞谱系分化的研究[J]. 中国骨与关节杂志, 2014(2):135-141. |
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