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Journal of Peking University (Health Sciences) ›› 2021, Vol. 53 ›› Issue (1): 34-39. doi: 10.19723/j.issn.1671-167X.2021.01.006

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Expression of cartilage oligomeric matrix protein in the synovial chondromatosis of the temporomandibular joint

HAN Wei-hua1,LUO Hai-yan2,GUO Chuan-bin1,NING Qi1,MENG Juan-hong1,Δ()   

  1. 1. Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China
    2. Department of Oral Pathology, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China
  • Received:2020-10-12 Online:2021-02-18 Published:2021-02-07
  • Contact: Juan-hong MENG E-mail:jhmeng@263.net

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Abstract:

Objective: To detect the expression of cartilage oligomeric matrix protein (COMP) in the synovial chondromatosis of the temporomandibular joint (TMJSC),and to discuss the possible interactions between COMP, transforming growth factor (TGF)-β3, TGF-β1 and bone morphogenetic protein-2 (BMP-2) in the development of this neoplastic disease.Methods: Patients in Peking University School and Hospital of Stomatology from January 2011 to February 2020 were selected, who had complete medical records, TMJSC was verified histologically after operation. The expressions of COMP, TGF-β3, TGF-β1 and BMP-2 in the TMJSC of the temporomandibular joint were detected by immunohistochemistry and quantitative real-time PCR (RT-PCR) at the protein level and mRNA level respectively, compared with the normal synovial tissue of temporomandibular joint. The histological morphology, protein expression and distribution of TMJSC tissues were observed microscopically, and the positive staining proteins were counted and scored. SPSS 22.0 statistical software was used to analyze the expression differences between the related proteins in TMJSC tissue and the normal synovial tissue of temporomandibular joint and to compare their differences. P<0.05 indicated that the difference was statistically significant.Results: Immunohistochemical results showed that the positive expression of COMP in TMJSC tissues was mostly found in synovial tissues and chondrocytes adjacent to synovial tissues, and the difference was statistically significant, compared with the normal temporomandibular joint synovial tissues. The positive expression of COMP was significantly different between recurrent TMJSC and non-recurrent ones. The positive expressions of TGF-β3, TGF-β1 and BMP-2 were higher than the normal synovial tissue, and were also mostly found in the synovial cells and adjacent chondrocytes, which was further confirmed by Western blot. According to the RT-PCR results, the expressions of COMP, TGF-β3, TGF-β1 and BMP-2 in TMJSC were higher than those in the normal synovial tissue.Conclusion: The expression of COMP in TMJSC of temporomandibular joint increased significantly, compared with the normal synovial tissue. There may be interactions between COMP and cytokines related to the proliferation and differentiation, like TGF-β3, TGF-β1 and BMP-2, which may play a potential role in the pathogenesis of TMJSC.

Key words: Temporomandibular joint, Synovial chondromatosis, Cartilage oligomeric matrix protein, Transforming growth factor, Bone morphogenetic protein-2

CLC Number: 

  • R782.6

Table 1

Primer sequence of mRNA templates"

mRNA template Primer sequence
COMP Forward: 5'-CAGATGGAGCAAACGTATTGG-3'
Reverse: 5'-GAAGACTTCACAGCCTTGAGTT-3'
TGF-β3 Forward: 5'-CTGTTGAGAAGAGAGTCCAACT-3'
Reverse: 5'-GATTTCCATCACCTCGTGAATG'-3'
TGF-β1 Forward: 5'-CTGTACATTGACTTCCGCAAG-3'
Reverse: 5'-TGTCCAGGCTCCAAATGTAG-3'
BMP-2 Forward: 5'-GACGTTGGTCAACTCTGTTAAC-3'
Reverse: 5'-GTCAAGGTACAGCATCGAGATA-3'

Figure 1

Histological findings of TMJSC (HE ×100) Synovial chondromatosis consists thickened synovial tissue (black arrow) and clustered chondrocytes (white arrow)."

Figure 2

Immunohistochemical findings of normal synovium and TMJSC Abbreviations as in Table 1. A-D, no positive expression of target protein was found in normal synovium of temporomandibular joint (IHC ×100). E-H, the positive expression of target proteins in synovial membrane and adjacent chondrocytes was higher (black arrow, IHC ×400)."

Table 2

Expression of related proteins in TMJSC by immunohistochemistry"

Proteins Distribution of protein expression score, n Positive rate/%,
Median
+ ++ +++ Total
COMP 0 19 33 52 47.80
TGF-β3 0 24 28 52 40.50
BMP-2 0 42 10 52 35.35
TGF-β1 2 37 13 52 15.00

Table 3

The difference of semi-quantitative protein expression of COMP by immunohistochemistry between recurrent and non-recurrent TMJSC"

Groups Semi-quantitative protein
expression of COMP, n		 Total
- + ++ +++
Recurrent TMJSC 0 0 0 4 4
Non-recurrent TMJSC 0 0 23 25 48
Normal synovium 3 0 0 0 3
Total 3 0 23 29 55

Figure 3

Expression difference of related proteins between TMJSC and normal synovium Abbreviations as in Table 1. A, mRNA expressions in normal synovium and TMJSC tissue (* P<0.05); B, the expressions of target proteins in normal synovium and TMJSC tissue (1-3, three different TMJSC patients’ synovium)."

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