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Journal of Peking University(Health Sciences) >

2024 , Vol. 56 >Issue 4: 687 - 692

DOI: https://doi.org/10.19723/j.issn.1671-167X.2024.04.023

Effects of PM2.5 and O3 sub-chronic combined exposure on ATP amount and ATPase activities in rat nasal mucosa

  • Tenglong YAN ,
  • Jiayu XU ,
  • Tian CHEN ,
  • Xin YANG ,
  • Weiwei WANG ,
  • Shupei ZHOU ,
  • Piye NIU ,
  • Guang JIA ,
  • Jiao XIA
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  • 1. Department of Occupational and Environmental Health Sciences, Peking University School of Public Health, Beijing 100191, China
    2. Beijing Institute of Occupational Disease Prevention and Treatment, Beijing 100093, China
    3. School of Public Health and the Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China
    4. Department of Otorhinolaryngology, Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
    5. Department of Laboratory Animal Science, Peking University Health Science Center, Beijing 100191, China

Received date: 2021-01-26

  Online published: 2024-07-23

Supported by

the National Natural Science Foundation of China(91643111);the National Natural Science Foundation of China(91743114)

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Abstract

Objective: To evaluate the effects of fine particle matter (PM2.5) and ozone (O3) combined exposure on adenosine triphosphate (ATP) amount and ATPase activities in nasal mucosa of Sprague Dawley (SD) rats. Methods: Twenty male SD rats were divided into control group (n=10) and exposure group (n=10) by random number table method. The rats were fed in the conventional clean environment and the air pollutant exposure system established by our team, respectively, and exposed for 208 d. During the exposure period, the concentrations of PM2.5 and O3 in the exposure system were monitored, and a comprehensive assessment of PM2.5 and O3 in the exposure system was conducted by combining self-measurement and site data. On the 208 d of exposure, the core, liver, spleen, kidney, testis and other major organs and nasal mucosal tissues of the rats were harvested. Each organ was weighed and the organ coefficient calculated. The total amount of ATP was measured by bioluminescence, and the activities of Na+-K+ -ATPase and Ca2+ -ATPase were detected by spectrophotometry. The t test of two independent samples was used to compare the differences among the indicator groups. Results: From the 3rd week to the end of exposure duration, the body weight of the rats in the exposure group was higher than that in the control group (P < 0.05), and there was no significant difference in organ coefficients between the two groups. The average daily PM2.5 concentration in the exposure group was (30.68±19.23) μg/m3, and the maximum 8 h ozone concentration (O3-8 h) was (82.45±35.81) μg/m3. The chemiluminescence value (792.4±274.1) IU/L of ATP in nasal mucosa of the rats in the exposure group was lower than that in the control group (1 126.8±218.1) IU/L. The Na+-K+-ATPase activity (1.53±0.85) U/mg in nasal mucosa of the rats in the exposure group was lower than that in the control group (4.31±1.60) U/mg (P < 0.05). The protein content of nasal mucosa in the control group and the exposure group were (302.14±52.51) mg/L and (234.58±53.49) mg/L, respectively, and the activity of Ca2+-ATPase was (0.81±0.27) U/mg and (0.99±0.73) U/mg, respectively. There was no significant difference between the groups. Conclusion: The ability of power capacity decreased in the rat nasal mucossa under the sub-chronic low-concentration exposure of PM2.5 and O3.

Key words： Fine particle matter; Ozone; Adenosine triphosphate

Cite this article

Tenglong YAN , Jiayu XU , Tian CHEN , Xin YANG , Weiwei WANG , Shupei ZHOU , Piye NIU , Guang JIA , Jiao XIA . Effects of PM2.5 and O3 sub-chronic combined exposure on ATP amount and ATPase activities in rat nasal mucosa[J]. Journal of Peking University(Health Sciences), 2024 , 56(4) : 687 -692 . DOI: 10.19723/j.issn.1671-167X.2024.04.023

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