Email Alert | RSS

Journal of Peking University (Health Sciences) ›› 2024, Vol. 56 ›› Issue (4): 687-692. doi: 10.19723/j.issn.1671-167X.2024.04.023

Previous Articles     Next Articles

Effects of PM2.5 and O3 sub-chronic combined exposure on ATP amount and ATPase activities in rat nasal mucosa

Tenglong YAN1,2,Jiayu XU1,Tian CHEN3,Xin YANG3,Weiwei WANG4,Shupei ZHOU5,Piye NIU3,Guang JIA1,Jiao XIA4,*()   

  1. 1. Department of Occupational and Environmental Health Sciences, Peking University School of Public Health, Beijing 100191, China
    2. Beijing Institute of Occupational Disease Prevention and Treatment, Beijing 100093, China
    3. School of Public Health and the Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China
    4. Department of Otorhinolaryngology, Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
    5. Department of Laboratory Animal Science, Peking University Health Science Center, Beijing 100191, China
  • Received:2021-01-26 Online:2024-08-18 Published:2024-07-23
  • Contact: Jiao XIA E-mail:jiaoxia0322@msn.com
  • Supported by:
    the National Natural Science Foundation of China(91643111);the National Natural Science Foundation of China(91743114)

RICH HTML

   

PDF (PC)

Abstract:

Objective: To evaluate the effects of fine particle matter (PM2.5) and ozone (O3) combined exposure on adenosine triphosphate (ATP) amount and ATPase activities in nasal mucosa of Sprague Dawley (SD) rats. Methods: Twenty male SD rats were divided into control group (n=10) and exposure group (n=10) by random number table method. The rats were fed in the conventional clean environment and the air pollutant exposure system established by our team, respectively, and exposed for 208 d. During the exposure period, the concentrations of PM2.5 and O3 in the exposure system were monitored, and a comprehensive assessment of PM2.5 and O3 in the exposure system was conducted by combining self-measurement and site data. On the 208 d of exposure, the core, liver, spleen, kidney, testis and other major organs and nasal mucosal tissues of the rats were harvested. Each organ was weighed and the organ coefficient calculated. The total amount of ATP was measured by bioluminescence, and the activities of Na+-K+ -ATPase and Ca2+ -ATPase were detected by spectrophotometry. The t test of two independent samples was used to compare the differences among the indicator groups. Results: From the 3rd week to the end of exposure duration, the body weight of the rats in the exposure group was higher than that in the control group (P < 0.05), and there was no significant difference in organ coefficients between the two groups. The average daily PM2.5 concentration in the exposure group was (30.68±19.23) μg/m3, and the maximum 8 h ozone concentration (O3-8 h) was (82.45±35.81) μg/m3. The chemiluminescence value (792.4±274.1) IU/L of ATP in nasal mucosa of the rats in the exposure group was lower than that in the control group (1 126.8±218.1) IU/L. The Na+-K+-ATPase activity (1.53±0.85) U/mg in nasal mucosa of the rats in the exposure group was lower than that in the control group (4.31±1.60) U/mg (P < 0.05). The protein content of nasal mucosa in the control group and the exposure group were (302.14±52.51) mg/L and (234.58±53.49) mg/L, respectively, and the activity of Ca2+-ATPase was (0.81±0.27) U/mg and (0.99±0.73) U/mg, respectively. There was no significant difference between the groups. Conclusion: The ability of power capacity decreased in the rat nasal mucossa under the sub-chronic low-concentration exposure of PM2.5 and O3.

Key words: Fine particle matter, Ozone, Adenosine triphosphate

CLC Number: 

  • R122.7

Figure 1

Animal body mass monitoring and general toxicity evaluation during exposure A, animal body mass monitoring during exposure, the yellow indicates statistically significant difference between groups (P < 0.05); B, comparison of organ coefficients between the two groups."

Figure 2

PM2.5 concentration in the exposure system and the monitoring station A, PM2.5 concentration in the exposure system and the monitoring station; B, average daily PM2.5 concentration in the exposed system based on self-measured data and monitoring site data. PM2.5, fine particle matter."

Figure 3

O3-8 h concentration in the exposure system and the monitoring station A, self-measured O3-8 h concentration in the exposure system and outdoor; B, O3-8 h average daily concentration in the exposed system based on self-measured data and monitoring site data. O3, ozone."

1 World Health Organization. Concentration of fine particle matter (PM2.5)[EB/OL]. (2019-08-28)[2020-08-12]. https://www.who.int/data/gho/data/indicators/indicator-details/GHO/concentrations-of-fine-particulate-matter-(pm2-5).
2 中华人民共和国生态环境部. 2015年中国生态环境状况公报[R/OL]. (2016-06-02)[2020-10-08]. https://www.mee.gov.cn/ywdt/tpxw/201606/t20160602_353077.shtml.
3 中华人民共和国生态环境部. 2016年中国生态环境状况公报[R/OL]. (2017-06-05)[2020-11-02]. https://www.mee.gov.cn/gkml/sthjbgw/qt/201706/t20170605_415442.htm.
4 中华人民共和国生态环境部. 2017年中国生态环境状况公报[R/OL]. (2018-05-31)[2020-10-05]. https://www.mee.gov.cn/hjzl/sthjzk/zghjzkgb/201805/P020180531534645032372. pdf.
5 中华人民共和国生态环境部. 2018年中国生态环境状况公报[R]. (2019-05-29)[2020-11-07]. https://www.mee.gov.cn/ywdt/tpxw/201905/t20190529_704841.shtml.
6 孙宏发. 基于CT图像的真实成人上呼吸道内气流特性及颗粒物沉积研究[D]. 陕西: 西安建筑科技大学, 2015.
7 Sundukov YN . First record of the ground beetle Trechoblemus postilenatus (Coleoptera, Carabidae) in Primorskii krai[J]. Far Eastern Entomologist, 2006, 165 (4): 16.
8 World Health Organization. Exposure to ambient air pollution from particulate matter for 2016[R/OL]. (2018-04-02)[2020-10-10]. https://cdn.who.int/media/docs/default-source/air-quality-database/aqd-2018/aap_exposure_apr2018_final.pdf?sfvrsn=86d2fad9_3.
9 Cabrera M , Garzón García B , Moreno-Grau S , et al. Association between seasonal allergic rhinitis and air pollution, meteorological factors, and grass pollen counts in madrid (1996 and 2009)[J]. J Investig Allergol Clin Immunol, 2019, 29 (5): 371- 377.
doi: 10.18176/jiaci.0368
10 Brant T , Brant T , Yoshida C , et al. Mucociliary clearance, airway inflammation and nasal symptoms in urban motorcyclists[J]. Clinics, 2014, 69 (12): 867- 870.
doi: 10.6061/clinics/2014(12)13
11 Jia JX , Xia J , Zhang RX , et al. Investigation of the impact of PM2.5 on the ciliary motion of human nasal epithelial cells[J]. Chemosphere, 2019, 233 (5): 309- 318.
12 Li FX , Ma HH , Liu J . Pyrethroid insecticide cypermethrin modulates gonadotropin synthesis via calcium homeostasis and ERK1/2 signaling in LβT2 mouse pituitary cells[J]. Toxicol Sci, 2018, 162 (1): 43- 52.
doi: 10.1093/toxsci/kfx248
13 Dizier MH , Margaritte-Jeannin P , Pain L , et al. Interactive effect between ATPase-related genes and early-life tobacco smoke exposure on bronchial hyper-responsiveness detected in asthma-ascertained families[J]. Thorax, 2019, 74 (3): 254- 260.
doi: 10.1136/thoraxjnl-2018-211797
14 阎腾龙, 夏交, 胥嘉钰, 等. 北京大气污染物暴露对大鼠嗅觉的影响[J]. 中华预防医学杂志, 2020, 54 (7): 774- 778.
doi: 10.3760/cma.j.cn112150-20200508-00699
15 Ostrowski LE , Bennett WD . Cilia and mucociliary clearance[J]. Encycl Respir Med, 2006, 10 (1101): 466- 470.
16 Angelis N , Spyratos D , Domvri K , et al. Effect of ambient ozone exposure assessed by individual monitors on nasal function and exhaled NO among school children in the area of Thessaloniki, Greece[J]. J Occup Environ Med, 2017, 59 (6): 509- 515.
doi: 10.1097/JOM.0000000000001011
17 宋晓玲, 邢志敏, 余力生. 大气可吸入颗粒物对变应性鼻炎大鼠鼻黏膜的影响[J]. 中国耳鼻咽喉头颈外科, 2008, 15 (4): 223- 226.
18 Wagner JG , Hotchkiss JA , Harkema JR . Enhancement of nasal inflammatory and epithelial responses after ozone and allergen coexposure in Brown Norway rats[J]. Toxicol Sci, 2002, 67 (2): 284- 294.
doi: 10.1093/toxsci/67.2.284
19 Gudis D , Zhao KQ , Cohen NA . Acquired cilia dysfunction in chronic rhinosinusitis[J]. Am J Rhinol Allergy, 2012, 26 (1): 1- 6.
doi: 10.2500/ajra.2012.26.3716
20 Andreoli SM , Schlosser RJ , Wang LF , et al. Adenoid ciliostimulation in children with chronic otitis media[J]. Otolaryngol Head Neck Surg, 2013, 148 (1): 135- 139.
doi: 10.1177/0194599812462664
21 Guo ZQ , Hong ZC , Dong WY , et al. PM2.5-induced oxidative stress and mitochondrial damage in the nasal mucosa of rats[J]. Int J Environ Res Public Health, 2017, 14 (2): 134.
doi: 10.3390/ijerph14020134
22 中华人民共和国生态环境部. 环境空气质量标准[EB/OL]. (2016-01-01)[2020-10-11]. https://www.mee.gov.cn/ywgz/fgbz/bz/bzwb/dqhjbh/dqhjzlbz/201203/t20120302_224165.shtml.
23 Zhang ZL , Dong B , Chen GB , et al. Ambient air pollution and obesity in school-aged children and adolescents: A multicenter study in China[J]. Sci Total Environ, 2021, 771, 144583.
doi: 10.1016/j.scitotenv.2020.144583
24 Jin XT , Su H , Ding G , et al. Exposure to ambient fine particles causes abnormal energy metabolism and ATP decrease in lung tissues[J]. Chemosphere, 2019, 224, 29- 38.
doi: 10.1016/j.chemosphere.2019.02.116
25 de Sá MC , Nakagawa NK , de André CDS , et al. Aerobic exercise in polluted urban environments: Effects on airway defense mechanisms in young healthy amateur runners[J]. J Breath Res, 2016, 10 (4): 1- 9.
26 Skou JC , Esmann M . The Na+-K+-ATPase[J]. J Bioenerg Biomembr, 1992, 24 (3): 249- 261.
27 Anbarasi K , Vani G , Balakrishna K , et al. Effect of bacoside A on membrane-bound ATPases in the brain of rats exposed to cigarette smoke[J]. J Biochem Mol Toxic, 2005, 19 (1): 59- 65.
28 Demir TA , Akar T , Akyüz F , et al. Nickel and cadmium concentrations in plasma and Na+/ K+ATPase activities in erythrocyte membranes of the people exposed to cement dust emissions[J]. Environ Monit Assess, 2005, 104 (1/2/3): 437- 444.
29 Li RJ , Kou XJ , Geng H , et al. Effect of ambient PM2.5 on lung mitochondrial damage and fusion/fission gene expression in rats[J]. Chem Res Toxicol, 2015, 28 (3): 408- 418.
30 Giraud F , Claret M , Garay R . Interactions of cholesterol with the Na pump in red blood cells[J]. Nature, 1976, 264 (5587): 646- 648.
31 Duchnowicz P , Koter M . Damage to the erythrocyte membrane caused by chlorophenoxyacetic herbicides[J]. Cel Mol Biol Lett, 2003, 8 (1): 25- 30.
[1] ZHANG Hong,DONG Ji-yuan,WANG Jian-jun,FAN Lin-xia,QU Qiang,LIU Yang. Short-term effects and seasonal variation of ozone on daily hospital outpatient visits for childhood asthma in Lanzhou [J]. Journal of Peking University (Health Sciences), 2022, 54(2): 227-235.
[2] Jia-hui CHEN,Da-yu HU,Xu JIA,Wei NIU,Fu-rong DENG,Xin-biao GUO. Monitoring metrics for short-term exposure to ambient ozone and pulmonary function and airway inflammation in healthy young adults [J]. Journal of Peking University (Health Sciences), 2020, 52(3): 492-499.
[3] FANG Wei-gang, TIAN Xin-xia. Identification of a new pro-invasion factor in tumor microenvironment: progress in function and mechanism of extracellular ATP [J]. Journal of Peking University(Health Sciences), 2017, 49(2): 188-195.
[4] GAO Xin, SHANG Jing, YANG Jing-Lin, LI Qian, CHEN Tian, PANG Yuan-Jie, ZHANG Wen-Xiao, LUAN Xian-Guo, ZHU Tong, JIA Guang. Comparison of genetic damage in mice exposed to black carbon and ozone-oxidized black carbon [J]. Journal of Peking University(Health Sciences), 2014, 46(3): 400-404.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright © Journal of Peking University (Health Sciences), All Rights Reserved.
Powered by Beijing Magtech Co. Ltd