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Journal of Peking University(Health Sciences) ›› 2019, Vol. 51 ›› Issue (2): 239-244. doi: 10.19723/j.issn.1671-167X.2019.02.007

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Effects of sulfur dioxide on alveolar macrophage apoptosis in acute lung injury induced by limb ischemia/reperfusion in rats

Yan-rui ZHAO,Yang LIU,Dong WANG,Wen-rui LV,Jun-lin ZHOU()   

  1. Department of Orthopedics, Beijing Chao-yang Hospital, Capital Medical University, Beijing 100020, China
  • Received:2017-04-06 Online:2019-04-18 Published:2019-04-26
  • Contact: Jun-lin ZHOU E-mail:doctorzyr@163.com
  • Supported by:
    the National Natural Science Foundation of China(81070050);Beijing Natural Science Foundation(7152061)

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Abstract:

Objective: To investigate the effect of sulfur dioxide (SO2) on the apoptosis of alveolar macrophage (AM) in lung protection of limb ischemia/reperfusion (I/R) induced acute lung injury (ALI), and to find a new target for the control of inflammatory response.Methods: Twenty pathogen-free, adult male Sprague-Dawley (SD) rats (180-230 g) were used in this study. Five rats were to be used for limb ischemia/reperfusion, then plasma was extracted as ischemia/reperfusion serum stimulation. Fifteen rats were to be used for extracting AM by bronchoalveolar lavage. The AM was isolated and cultured, then the cell count was adjusted to 1×10 6/mL, and randomly divided into the following 4 groups (n=6): control group, I/R group, SO2 group, and I/R+SO2 group. The I/R group was given ischemia/reperfusion serum (500 μg/L) to stimulate 6 h; the SO2 group was given an SO2 donor, Na2SO3/NaHSO3 [(0.54 mmol/kg) / (0.18 mmol/kg)]; and the I/R+SO2 group was given the same ischemia/reperfusion serum and Na2SO3/NaHSO3 at the same time. The level of mitochondrial membrane potential, the state of mitochondrial permeability transition pore (mPTP), the rate of AM apoptosis, the expression of Bcl-2 and Caspase-3 proteins were detected by flow cytometry, microplate reader and Western blotting.Results: Compared with the control group, in the I/R group, the ratio of red to green fluorescence and the absorbance decreased significantly, the percentage of apoptotic cells increased obviously, the apoptotic rate was 43.81%±2.40%, Caspase-3 protein expression increased, Bcl-2 protein expression decreased. While compared with the I/R group, in the I/R+SO2 group, the ratio of red to green fluorescence and the absorbance increased significantly; the apoptotic rate decreased to 37.01%±1.93%, Caspase-3 protein expression decreased, Bcl-2 protein expression increased.Conclusion: Exo-genous SO2 has the effect of accelerating AM apoptosis by stimulating mPTP to open and mitochondrial membrane potential to decrease; besides, exogenous SO2 could stimulate AM to secrete more anti-inflammatory cytokines and less inflammatory cytokines. In conclusion, exogenous SO2 can reduce macrophage apoptosis by inhibiting mitochondrial pathways.

Key words: Sulfur dioxide, Reperfusion injury, Acute lung injury, Macrophages, alveolar

CLC Number: 

  • R364.12

Table 1

Effect of exogenous SO2 on mitochondrial membrane potential and mPTP opening of AM (n=8)"

Group Ratio (Red/Green) Absorbance
Control 4.94±0.26 0.45±0.03
SO2 5.00±0.29 0.44±0.02
I/R 1.75±0.24* 0.24±0.02*
I/R+SO2 2.12±0.15# 0.27±0.03#

Figure 1

Detection of AM apoptosis by Annexin V-FITC and PI double-label flow cytometry A, representative flow cytometric dot plots. X-axis, Annexin-V staining; Y-axis, PI staining. Q1, detection error within the permitted range; Q2, late apoptotic cells and necrotic cells; Q3, early apoptotic cells; Q4, normal living cells (The apoptotic cells are Q2+Q3). B, quantitation of apoptotic cells population as shown in figure A. All the results are expressed as x ? ±s (n=8). *P<0.01 vs. control group; #P<0.05 vs. I/R group. "

Figure 2

The expression of Bcl-2 and Caspase-3 proteins of AM in different groups A, representative bands of Western blots; B, bar graphs show the relative expression of Bcl-2 and Caspase-3 proteins of AM in different groups. All the results are expressed as x ? ±s (n=3). *P<0.01 vs. control group; #P<0.05 vs. I/R group. "

[1] Steiger AK, Yang Y, Royzen M , et al. Bio-orthogonal “click-and-release” donation of caged carbonyl sulfide (COS) and hydrogen sulfide (H2S)[J]. Chem Commun (Camb), 2017,53(8):1378-1380.
doi: 10.1039/C6CC09547J
[2] Steiger AK, Pardue S, Kevil CG , et al. Self-immolative thiocarbamates provide access to triggered H2S donors and analyte replacement fluorescent probes[J]. J Am Chem Soc, 2016,138(23):7256-7259.
doi: 10.1021/jacs.6b03780
[3] Aggarwal NR, D’Alessio FR, Tsushima K , et al. Moderate oxygen augments lipopolysaccharide-induced lung injury in mice[J]. Am J Physiol Lung Cell Mol Physiol, 2010,298(3):L371-L381.
doi: 10.1152/ajplung.00308.2009
[4] Howard KM . Differential expression of platelet-activating factor acetylhydrolase in lung macrophages[J]. Am J Physiol Lung Cell Mol Physiol, 2009,297(6):L1141-L1150.
doi: 10.1152/ajplung.00022.2009
[5] Z’Graggen BR, Tornic J, Muller-Edenborn B , et al. Acute lung injury: apoptosis in effector and target cells of the upper and lower airway compartment[J]. Clin Exp Immunol, 2010,161(2):324-331.
[6] Meng Z, Liu Y . Cell morphological ultrastructural changes in va-rious organs from mice exposed by inhalation to sulfur dioxide[J]. Inhal Toxicol, 2007,19(6-7):543-551.
doi: 10.1080/08958370701271373
[7] Ubuka T, Yuasa S, Ohta J , et al. Formation of sulfate from L-cysteine in rat liver mitochondria[J]. Acta Med Okayama, 1990,44(2):55-64.
[8] 赵彦瑞, 刘洋, 王东 , 等. PI3KAkt和JAK2STAT3信号转导通路在SO2抗大鼠肢体缺血再灌注致急性肺损伤中的作用[J]. 中华病理生理杂志, 2015,31(11):2076-2082.
[9] Huang XL, Liu Y, Zhou JL , et a1. Role of sulfur dioxide in acute lung injury following limb ischemia/reperfusion in rats[J]. J Biochem Mol Toxicol, 2013,27(8):389-397.
doi: 10.1002/jbt.2013.27.issue-8
[10] Zhao YR, Wang D, Liu Y , et al. The PI3K/Akt, p38MAPK, and JAK2/STAT3 signaling pathways mediate the protection of SO2 against acute lung injury induced by limb ischemia/reperfusion in rats[J]. J Physiol Sci, 2016,66(3):229-239.
doi: 10.1007/s12576-015-0418-z
[11] Cohen SM, Siddiqi FA, Darakchiev B , et al. Attenuation of acute lung injury caused by hind-limb ischemia-reperfusion injury by butyrolactone anti-inflammatory agent FLl003[J]. J Trauma, 1997,43(2):247-252.
doi: 10.1097/00005373-199708000-00007
[12] Javadov S, Choi A, Rajapurohitam V , et al. NHE-1 inhibition-induced cardioprotection against ischaemia/reperfusion is associa-ted with attenuation of the mitochondrial permeability transition[J]. Cardiovasc Res, 2008,77(2):416-424.
[13] Kaltenbach JP, Jennings RB . Metabolism of ischemic cardiac muscle[J]. Circ Res, 1960(8):207-213.
[14] Ward PA . Editorial commentary: New strategies for treatment of humans with acute lung injury/acute respiratory distress syndrome[J]. Clin Infect Dis, 2015,60(4):596-597.
doi: 10.1093/cid/ciu892
[15] Tauber AI . Metchnikoff and the phagocytosis theory[J]. Nat Rev Mol Cell Biol, 2003,4(11):897-901.
doi: 10.1038/nrm1244
[16] Ovchinnikov, Dmitry A . Macrophages in the embryo and beyond: Much more than just giant phagocytes[J]. Genesis, 2008,46(9):447-462.
doi: 10.1002/dvg.v46:9
[17] Yong Y, Matthew S, Wittwer J , et al. Dichamanetin inhibits cancer cell growth by affecting ROS-related signaling components through mitochondrial-mediated apoptosis[J]. Anticancer Res, 2013,33(12):5349-5355.
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