Journal of Peking University(Health Sciences) ›› 2015, Vol. 47 ›› Issue (1): 42-46.

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Spatiotemporal expression pattern of E-cadherin and P-cadherin during mouse tooth development

ZHAO Shuang-yun1,2, LIU Ming2, ZHAO Yu-ming1, LIN Qing-jie2,3, WANG Xiu-ping2△, GE Li-hong1△   

  1. (1. Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China; 2. Department of Developmental Biology, Harvard School of Dental Medicine, Boston 02115, USA; 3. Department of Implantation, School of Stomatology, Shandong University, Jinan 250012, China)
  • Online:2015-02-18 Published:2015-02-18

Abstract: Objective: To investigate the expression patterns of E-cadherin and P-cadherin in murinetooth germs at early developmental stages. Methods: Mandible samples of CD1 mice from embryonic day 12.5 to postnatal day 3.5 were collected. The expressions of E-cadherin and P-cadherin in murine mandibular first molar germs were detected by immunofluorescence and observed under confocal fluorescence microscope. HE staining was performed for tissue morphology. Results: Both E-cadherin and P-cadherin were widely expressed in the epithelial tissues through early developmental stages. The E-cadherin expression was increased in polarizing pre-ameloblasts, whereas the P-cadherin expression declined. The expression of the P-cadherin could be detected in epithelial tissues before bud stage, and expressed in mature ameloblasts at secretory stage. Conclusion: The E-cadherin and P-cadherin expressed in different spatiotemporal expression patterns, indicating their individual functions during tooth development. P-cadherin might function in the secretion and mineralization of enamel.

Key words: Cadherins, Tooth germ, Fluorescent antibody technique

[1] TIAN Jing,QIN Man,CHEN Jie,XIA Bin. Early loss of primary molar and permanent tooth germ caused by the use of devitalizer during primary molar root canal therapy: Two cases report [J]. Journal of Peking University (Health Sciences), 2022, 54(2): 381-385.
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