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北京大学学报（医学版） ›› 2025, Vol. 57 ›› Issue (1): 73-77. doi: 10.19723/j.issn.1671-167X.2025.01.011

• 论著 • 上一篇    下一篇

氟保护漆抗牙根面酸蚀脱矿的耐久性体外研究

田洪琰1, 蔡雪2,*(), 王晓燕2   

  1. 1. 北京大学口腔医学院·口腔医院门诊部，国家口腔医学中心，国家口腔疾病临床医学研究中心，口腔生物 材料和数字诊疗装备国家工程研究中心，北京 100081
    2. 北京大学口腔医学院·口腔医院牙体牙髓科，国家口腔医学中心，国家口腔疾病临床医学研究中心，口腔生物 材料和数字诊疗装备国家工程研究中心，北京 100081
  • 收稿日期:2024-09-26 出版日期:2025-02-18 发布日期:2025-01-25
  • 通讯作者: 蔡雪 E-mail:caixue@bjmu.edu.cn
  • 基金资助:
    国家自然科学基金(51602008)

Durability of the anti-demineralization effects of fluoride varnish on dental root surfaces: An in vitro study

Hongyan TIAN1, Xue CAI2,*(), Xiaoyan WANG2   

  1. 1. First Clinical Division, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, China
    2. Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, China
  • Received:2024-09-26 Online:2025-02-18 Published:2025-01-25
  • Contact: Xue CAI E-mail:caixue@bjmu.edu.cn
  • Supported by:
    the National Natural Science Foundation of China(51602008)

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摘要：

目的: 研究在牙根面应用氟保护漆的抗酸蚀脱矿作用的耐久性。方法: 制备人离体牙冠部和根部样本，涂布多乐氟形成保护膜，对保护膜-牙本质界面进行扫描电镜观察和氟元素含量能谱分析，比较氟保护漆在冠和根上应用的差异。制备人离体牙根面样本，涂布多乐氟形成保护膜，在常温去离子水中放置14 d使其发生老化，然后用pH 4.5的乙酸脱矿4 d，再对保护膜-牙本质界面进行扫描电镜观察和氟元素含量能谱分析，并与未使用多乐氟的脱矿样本和使用多乐氟但未经过老化的脱矿样本进行对照分析。结果: 多乐氟的即刻渗透深度在冠部牙本质较强，在根部牙本质较弱。未使用多乐氟的对照组样本经酸蚀后发生明显脱矿。使用多乐氟的样本不经老化时，酸蚀后未发生脱矿，多乐氟中的氟元素渗透达界面下方(76.00±8.94) μm; 经过老化再酸蚀，保护膜结构破坏，但牙本质未发生脱矿，界面下方(5.00±3.53) μm处仍存留有氟元素。结论: 多乐氟在牙根面具有抗酸蚀脱矿作用，此种作用在失去保护膜结构后仍可发挥一段时间，具备一定的耐久性，可用于预防根面龋。

关键词: 外用氟化物, 多乐氟, 牙根龋, 耐久性

Abstract:

Objective: To study the durability of the anti-demineralization effects of fluoride varnish after being applied to dental root surfaces. Methods: Coronal and radicular dentin samples were prepared from extracted human teeth. Duraphat® (DP) was applied to the dentine surfaces to form a protective film. The film-dentin interfaces were observed by scanning electron microscopy (SEM) and the fluoride element was analyzed with energy dispersive spectrometer (EDS). Thus, the differences between applying DP on crowns and roots were compared. Radicular dentin samples were prepared and randomly divi-ded into four groups: (1) Blank: DP was not applied, and demineralized in acetic acid (pH 4.5) for 4 days; (2) Blank+aging: DP was not applied, the samples were put into deionized water for 14 days at room temperature, and then demineralized in acetic acid (pH 4.5) for 4 days; (3) DP: DP was applied and demineralized in acetic acid (pH 4.5) for 4 days; (4) DP+aging: DP was applied, the samples were put into deionized water for 14 days at room temperature, and then demineralized in acetic acid (pH 4.5) for 4 days. Finally, SEM observation and EDS analysis of fluoride content were performed on film-dentin interfaces to evaluate the degree of demineralization, the morphology of DP film, and the penetration of fluorine. Results: The immediate penetration depth of fluoride element from DP was deeper in the coronal dentin than that in radicular dentin. The samples in the blank and blank+aging groups demine-ralized significantly after acid etching. The DP group did not undergo demineralization, and the fluorine element penetrated to (76.00±8.94) μm below the interfaces. The structure of the protective film in the DP+aging group was damaged, but the underneath dentin did not undergo demineralization. The fluorine element still remained at a depth of (5.00±3.53) μm below the interfaces. Conclusion: DP has an anti-demineralization effect on the root surface, and this effect can still be exerted for a period of time after losing the structure of protective film. It has the ability to prevent root caries and a certain durability.

Key words: Topical fluorides, Duraphat, Root caries, Durability

中图分类号: 

  • R783.1

图1

人离体牙冠部及根部牙本质样本制备及扫描电镜观察位置示意图"

表1

实验分组和处理方法"

BlankDPBlank+agingDP+aging
DP was appliedDP was applied
Water aging for 14 dWater aging for 14 d
Demineralized for 4 dDemineralized for 4 dDemineralized for 4 dDemineralized for 4 d

图2

DP保护膜在冠部和根部的扫描电镜结果"

图3

根面多乐氟保护膜老化酸蚀后扫描电镜图"

1 冯希平. 中国居民口腔健康状况——第四次中国口腔健康流行病学调查报告[C]//中华口腔医学会口腔预防医学专业委员会. 2018年中华口腔医学会第十八次口腔预防医学学术年会论文汇编. 西安: [出版者不详], 2018: 13-14.
2 台保军. 中国居民口腔健康状况及防控策略第四次全国口腔健康流行病学调查结果解读[C]//中华口腔医学会老年口腔医学专业委员会. 第十三次全国老年口腔医学学术年会论文汇编. 武汉: [出版者不详], 2018: 16-17.
3 Cai J , Palamara J , Manton DJ , et al. Status and progress of treatment methods for root caries in the last decade: A literature review[J]. Aust Dent J, 2018, 63 (1): 34- 54.
doi: 10.1111/adj.12550
4 管兆兰, 钱雅婧, 王威. 高频率使用多乐氟预防正畸患者牙脱矿的效果研究[J]. 口腔医学, 2023, 43 (2): 141- 144.
5 闻静, 周春华, 许妍. 光学相干断层成像检测牙釉质表层脱矿与再矿化的实验研究[J]. 北京口腔医学, 2019, 27 (3): 143- 146.
6 杨茜, 付梦辰, 王慧慧, 等. Duraphat与Fluor Protector抑制固定正畸矫治中牙釉质脱矿的比较研究[J]. 口腔医学, 2019, 39 (11): 1022- 1026.
7 Jiang W , Wang G , Wu W , et al. The effect of calcium phosphate ion clusters in enhancing enamel conditions versus Duraphat and Icon[J]. Aust Endod J, 2023, 49 (Suppl 1): 46- 57.
8 Casimiro-Iriarte SA , Chiok-Ocana LR . Fluoride release from fluo-ride varnishes exposed to commonly consumed beverages: An in vitro study[J]. J Clin Exp Dent, 2023, 15 (3): e187- e194.
9 舒泉湧, 麻纪斌, 邢建峰, 等. 多乐氟中氟含量及体外释放度研究[J]. 合成材料老化与应用, 2017, 46 (2): 79- 81.
10 Hosoya Y , Ando S , Yamaguchi K , et al. Quality of the interface of primary tooth dentin bonded with antibacterial fluoride-releasing adhesive[J]. J Dent, 2010, 38 (5): 423- 430.
doi: 10.1016/j.jdent.2010.02.001
11 Ma S , Imazato S , Chen J , et al. Effects of a coating resin containing S-PRG filler to prevent demineralization of root surfaces[J]. Dent Mater J, 2012, 31 (6): 909- 915.
doi: 10.4012/dmj.2012-061
12 Carey CM . Focus on fluorides: Update on the use of fluoride for the prevention of dental caries[J]. J Evid Based Dent Pract, 2014, 14 (Suppl): 95- 102.
13 Joves GJ , Inoue G , Nakashima S , et al. Mineral density, morphology and bond strength of natural versus artificial caries-affected dentin[J]. Dent Mater J, 2013, 32 (1): 138- 143.
doi: 10.4012/dmj.2012-243
14 Arends J , Duschner H , Ruben JL . Penetration of varnishes into demineralized root dentine in vitro[J]. Caries Res, 1997, 31 (3): 201- 205.
doi: 10.1159/000262399
15 Asian J , Quenta E , Castillo J . Do viscosity and wettability of fluoride varnishes affect their fluoride release?[J]. J Clin Exp Dent, 2021, 13 (3): e221- e226.
16 Cochrane NJ , Shen P , Yuan Y , et al. Ion release from calcium and fluoride containing dental varnishes[J]. Aust Dent J, 2014, 59 (1): 100- 105.
doi: 10.1111/adj.12144
17 Ekstrand K , Martignon S , Holm-Pedersen P . Development and evaluation of two root caries controlling programmes for home-based frail people older than 75 years[J]. Gerodontology, 2008, 25 (2): 67- 75.
doi: 10.1111/j.1741-2358.2007.00200.x
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ISSN 1671-167X
CN 11-4691/R
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