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Journal of Peking University (Health Sciences) ›› 2022, Vol. 54 ›› Issue (1): 18-22. doi: 10.19723/j.issn.1671-167X.2022.01.004

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Gene polymorphisms of cytochrome B-245 alpha chain (CYBA) and cholesteryl ester transfer protein (CETP) and susceptibility to generalized aggressive periodontitis

ZHU Xiao-ling1,LI Wen-jing2,WANG Xian-e2,SONG Wen-li2,XU Li2,ZHANG Li2,FENG Xiang-hui2,LU Rui-fang2,SHI Dong2,MENG Huan-xin2,△()   

  1. 1. Department of Stomatology, Peking University People’s Hospital, Beijing 100044, China
    2. Department of Periodontology, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China
  • Received:2021-10-18 Online:2022-02-18 Published:2022-02-21
  • Contact: Huan-xin MENG E-mail:kqhxmeng@bjmu.edu.cn
  • Supported by:
    National Natural Science Foundations of China(30471882);National Natural Science Foundations of China(30973319);National Natural Science Foundations of China(81271149)

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Abstract:

Objective: To explore the correlation of cytochrome B-245 alpha chain (CYBA) rs4673 and cholesteryl ester transfer protein (CETP) rs12720922 polymorphisms with the susceptibility of gene-ralized aggressive periodontitis (GAgP). Methods: The study was a case-control trial. A total of 372 GAgP patients and 133 periodontally healthy controls were recruited. The CYBA rs4673 and CETP rs12720922 polymorphisms were detected by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Logistic regression models were used to analyze the correlation of CYBA rs4673 and CETP rs12720922 variants with the susceptibility of GAgP. The interaction between the two gene polymorphisms to the susceptibility of GAgP was analyzed by the likelihood ratio test. The interaction model adopted was the multiplication model. Results: The mean age of GAgP group and control group was (27.5±5.2) years and (28.8±7.1) years respectively. There was significant difference in age between the two groups (P<0.05). The gender distribution (male/female) was 152/220 and 53/80 respectively, and there was no significant difference between GAgP group and controls (P>0.05). For CYBA rs4673, the frequency of CT/TT genotype in the GAgP group was significantly higher than that in the controls [18.0% (66/366) vs. 10.6% (14/132), P<0.05]. After adjusting age and gender, the individuals with CT/TT genotype had a higher risk of GAgP (OR=1.86, 95%CI: 1.01-3.45, P<0.05), compared with CC genotype. There was no statistically significant difference in distributions of the CETP rs12720922 genotypes (GG, AA/AG) between GAgP patients and healthy controls (P>0.05). A significant interaction between CYBA rs4673 and CETP rs12720922 in the susceptibility to GAgP was observed. The GAgP risk of the individuals with CYBA rs4673 CT/TT and CETP rs12720922 GG genotypes was significantly increased (OR=3.25, 95%CI: 1.36-7.75, P<0.01), compared with those carrying CC and AA/AG genotypes. Conclusion: CYBA rs4673 CT/TT genotype is associated with GAgP susceptibility. There is a significant interaction between CYBA rs4673 CT/TT genotype and CETP rs12720922 GG genotype in the susceptibility of GAgP.

Key words: Aggressive periodontitis, Cytochrome B-245 alpha chain, Cholesterol ester transfer proteins, Gene polymorphism, Interaction

CLC Number: 

  • R781.42

Table 1

Comparison of demographic, clinical characteristics and genotype distributions in patients and controls"

Characteristic Control (n=133) GAgP (n=372) P
Age/years, $\bar{x}\pm s$ 28.8±7.1 27.5±5.2 0.029
Gender, n(%) 0.839
Male 53 (39.8) 152 (40.9)
Female 80 (60.2) 220 (59.1)
PD/mm, $\bar{x}\pm s$ 1.76±0.46 4.85±1.06 <0.001
BI, $\bar{x}\pm s$ 1.09±0.31 3.50±0.53 <0.001
AL/mm, $\bar{x}\pm s$ 0.00±0.01 4.45±1.52 <0.001
CYBA rs4673, n(%) 0.046
CC 118 (89.4) 300 (82.0)
CT+TT 14 (10.6) 66 (18.0)
CETP rs12720922, n(%) 0.145
GG 99 (75.0) 298 (81.0)
AA+AG 33 (25.0) 70 (19.0)

Table 2

Interaction analysis between CYBA rs4673 and CETP rs12720922 gene polymorphisms and susceptibility to generalized aggressive periodontitis"

Genotype n OR (95%CI) P2
CC+AA/AG 88 Ref.
CT/TT+AA/AG 14 0.60 (0.19-1.90) 0.384
CC+GG 329 1.17 (0.70-1.96) 0.555
CT/TT+GG 66 3.25 (1.36-7.75) 0.008
P1 0.033
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