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Journal of Peking University (Health Sciences) ›› 2021, Vol. 53 ›› Issue (1): 16-23. doi: 10.19723/j.issn.1671-167X.2021.01.004

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Association between root abnormalities and related pathogenic genes in patients with generalized aggressive periodontitis

LIU Jian,WANG Xian-e,LV Da,QIAO Min,ZHANG Li,MENG Huan-xin(),XU Li(),MAO Ming-xin   

  1. Department of Periodontology,Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China
  • Received:2020-09-29 Online:2021-02-18 Published:2021-02-07
  • Contact: Huan-xin MENG,Li XU E-mail:kqmeng@126.com;xulihome@263.net

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Abstract:

Objective: To explore the association between the abnormal root morphology and bone metabolism or root development related gene polymorphism in patients with generalized aggressive periodontitis.Methods: In the study, 179 patients with generalized aggressive periodontitis were enrolled, with an average age of (27.23±5.19) years, male / female = 67/112. The average number of teeth remaining in the mouth was (26.80±1.84). Thirteen single nucleotide polymorphisms (SNPs) of nine genes which related to bone metabolism and root development were detected by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Root abnormalities were identified using periapical radiographs. The abnormal root morphology included cone-rooted teeth,slender-root teeth,short-rooted teeth,curved-rooted teeth,syncretic-rooted molars,and molar root abnormalities. The number of teeth and incidence of abnormal root morphology in different genotypes of 13 SNPs were analyzed.Results: The constituent ratio of root with root abnormality in GAgP patients was 14.49%(695/4 798). The average number of teeth with abnormal root morphology in GAgP was (3.88±3.84). The average number of teeth with abnormal root morphology in CC, CT and TT genotypes in vitamin D receptor (VDR) rs2228570 was (4.66±4.10), (3.71±3.93) and (2.68±2.68). There was significant difference between TT genotype and CC genotype (t = 2.62, P =0.01). The average number of root morphological abnormalities in CC, CT and TT genotypes of Calcitotin Receptor (CTR) gene rs2283002 was (5.02±3.70),(3.43±3.95), and (3.05±3.12). The incidence of root morphological abnormalities in CC genotype was higher than that in the patients with CT and TT, and the difference was statistically significant(87.86% vs. 65.26% & 63.64%, P=0.006,adjusted OR =3.71,95%CI: 1.45-9.50). There was no significant difference in the incidence of abnormal root morphology between CT and TT genotypes.Conclusion: VDR rs2228570 and CTR rs2283002 may be associated with the occurrence of abnormal root morphology in patients with generalized aggressive periodontitis, which is worthy of further research.

Key words: Aggressive periodontitis, Root abnormality, Gene, Single nucleotide polymorphisms

CLC Number: 

  • R394.3

Table 1

13 SNPs from 9 candidate genes"

Gene SNPs
VDR rs2228570
CTR rs2283002, rs2374634
MMP-8 rs11225395
RANK rs11664594
DBP rs17467825, rs4588, rs7041
EGF rs2237051
BMP2 rs2273073
S100A8 rs3795391, rs3806232
Runx2/Cbfa1 rs6938177

Figure 1

Typical X-ray films of abnormal root morphology A, cone-rooted teeth of anterior; B, cone-rooted teeth of premolar; C, slender-root teeth; D, short-rooted teeth; E, curved-rooted teeth; F, syncretic-rooted molars; G, molar root abnormalities."

Table 2

Distribution of teeth with abnormal root morphology"

Item Teeth level, n (%)
Cone-rooted teeth of anterior 214 (31)
Cone-rooted teeth of premolar 160 (23)
Short-rooted teeth 106 (15)
Curved-rooted teeth 85 (12)
Slender-root teeth 53 (8)
Molar root abnormalities 39 (6)
Syncretic-rooted molars 37 (5)
Root abnormalities 695 (100)

Table 3

Different genotypes of 13 single nucleotide polymorphisms from 9 candidate genes and root morphology abnormalities in patients with GAgP"

SNPs Gene type GAgP, n(%) Cone-rooted teeth of anterior Teeth of root abnormalities
Median(P25, P75) P x-±s/Median(P25, P75) P
VDR rs2228570 CC 59 (33.91) 0 (0, 2) 4.66±4.10
CT 90 (51.72) 0 (0, 2) 0.02 3.71±3.93 0.01
TT 25 (14.37) 0 (0, 0) 2.68±2.68
CTR rs2283002 TT 22 (13.25) 0 (0, 1.25) 3.05±3.12
CT 95 (57.23) 0 (0, 2) 0.14 3.43±3.95 0.03
CC 49 (29.52) 0 (0, 2.50) 5.02±3.70
CTR rs2374634 TT 144 (83.72) 0 (0, 2) 3 (0, 6.75)
TC 25 (14.53) 0 (0, 1) 0.44 4 (0, 5) 0.75
CC 3 (1.74) 0 (0, 1) 6 (0, 7)
MMP-8 rs11225395 GG 73 (41.01) 0 (0, 2) 4.25±3.95
AG 88 (49.44) 0 (0, 1) 0.23 3.48±3.70 0.38
AA 17 (9.55) 0 (0, 3) 4.41±4.18
RANK rs11664594 TT 59 (34.30) 0 (0, 2) 4.17±4.26
AT 87 (50.58) 0 (0, 2) 0.80 4.18±3.80 0.21
AA 26 (15.12) 0 (0, 2) 2.73±2.86
DBP rs17467825 AA 77 (44.77) 0 (0, 2) 3.84±3.79
AG 73 (42.44) 0 (0, 2) 0.72 3.67±3.62 0.45
GG 22 (12.79) 0 (0, 2.50) 4.86±4.95
DBP rs4588 CC 76 (43.18) 0 (0, 2) 3 (0.25, 6)
CA 79 (44.89) 0 (0, 2) 0.51 4 (0, 6) 0.93
AA 21 (11.93) 0 (0, 3) 2 (0, 8.50)
DBP rs7041 TT 97 (55.43) 0 (0, 2) 3.84±3.60
GT 68 (38.86) 0 (0, 2) 0.39 3.99±4.23 0.75
GG 10 (5.71) 1.5 (0, 2.50) 4.80±3.82
EGF rs2237051 AA 73 (41.71) 0 (0, 1) 3.89±4.06
GA 90 (51.43) 0 (0, 2) 0.12 4.01±3.67 0.98
GG 12 (6.86) 0 (0, 2) 3.83±4.28
BMP2 rs2273073 TT 156 (90.17) 0 (0, 2) 0.81 3.96±3.83 0.97
GT 17 (9.83) 0 (0, 2) 4.00±4.26
GG - - - - -
S100A8 rs3795391 TT 136 (77.27) 0 (0, 2) 0.76 3.91±3.98 0.99
TC 40 (22.73) 0 (0, 2) 3.90±3.46
CC - - - - -
S100A8 rs3806232 TT 130 (73.03) 0 (0, 2) 0.41 3.79±4.00 0.63
CT 48 (26.97) 0 (0, 2) 4.10±3.47
CC - - - - -
Runx2/Cbfa1 CC 87 (49.71) 0 (0, 2) 3.44±3.73
rs6938177 TC 71 (40.57) 0 (0, 3) 0.01 4.65±4.03 0.041
TT 17 (9.71) 0 (0, 0.50) 2.47±2.62

Table 4

Incidence of root morphological abnormalities in different genotypes of 13 single nucleotide polymorphisms from 9 candidate genes"

SNPs Gene type Total Incidence of root abnormality
n(%) Adjusted OR(95%CI)
VDR rs2228570
CC 59 45 (76.27)
CT+TT 115 79 (68.70) 0.93 (0.56, 1.52)
CTR rs2283002
CC 49 43 (87.76) 3.71 (1.45, 9.50)*
CT+TT 117 76 (64.96)
CTR rs2374634
TT 144 105 (72.92)
TC+CC 28 19 (67.86) 1.28 (0.53, 3.06)
MMP-8 rs11225395
AA 17 12 (70.59)
GG+AG 161 115 (71.42) 1.04 (0.35, 3.12)
RANK rs11664594
TT 59 46 (77.93)
AT+AA 113 75 (66.37) 1.79 (0.87, 3.72)
DBP rs17467825
GG 22 16 (72.37)
AA+AG 150 107 (71.33) 0.93 (0.34, 2.54)
DBP rs4588
AA 21 15 (71.43)
CA+CC 155 79 (72.26) 1.04 (0.38, 2.86)
DBP rs7041
TT 97 73 (7526)
GT+GG 78 54 (69.23) 1.35 (0.69, 2.63)
EGF rs2237051
AA 73 48 (65.75)
GA+GG 102 79 (77.45) 0.56 (0.29, 1.09)
BMP2 rs2273073
TT 156 114 (73.08)
GT 17 12 (70.59) 1.13 (0.38, 3.40)
S100A8 rs3795391
TT 136 96 (70.59)
TC 40 30 (75.00) 0.80 (0.36, 1.79)
S100A8 rs3806232
TT 130 89 (68.46)
CT 48 38 (79.17) 0.57 (0.26, 1.26)
Runx2/Cbfa1 rs6938177 CC 87 60 (68.97)
TC+TT 88 79 (73.86) 0.79 (0.41, 1.52)

Figure 2

Periapical radiographs of a GAgP patient with VDR rs2228570 CC genotype Male, 21 years, VDR rs2228570 CC genotype;Cone-rooted teeth of anterior: 11, 21, 32, 31, 41, 42; Curved-rooted teeth: 12, 22; Molar root abnormalities: 36, 46."

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