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Journal of Peking University(Health Sciences) ›› 2017, Vol. 49 ›› Issue (5): 893-898. doi: 10.3969/j.issn.1671-167X.2017.05.027

• Article • Previous Articles     Next Articles

Anatomy and histology characteristics of lymph node in nude mice

SUN Rui1, GAO Bo2, GUO Chuan-bin3   

  1. 1. Department of Oral and Maxillofacial Surgery, Shanxi Provincial People’s Hospital, Taiyuan 030012, China;
    2. Department of Oral and Maxillofacial Surgery, Shanxi Medical University School of Stomotology, Taiyuan 030001, China;
    3. Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology &
    National Engineering Laboratory for Digital and Material Technology of Stomatology &
    Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China
  • Received:2017-04-27 Online:2017-10-18 Published:2017-10-18
  • Supported by:
    Supported by the National Natural Science Foundation of China (30872899) and the Natural Science Foundation of Shanxi Province (2011011037)

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Abstract: Objective: To compare the differences of anatomical and histological characteristics of lymph nodes between BALB/c nude mice and BALB/c mice. Methods: Firstly, twenty BALB/c nude mice and twenty BALB/c mice were dissected by using a surgical microscope. Secondly, the differences of T cells and B cells at the lymph node were compared by the expressions of CD3 and CD20 immunohistochemistry dyes. Results: There were, on average, 23 nodes per mouse contained within the large lymph node assembly in the BALB/c nude mouse. The anatomical features of the lymph node distribution in the nude mice were mainly found in the neck with relatively higher density. There were two lymph nodes both in the submandible lymph nodes group and in the superficial cervical lymph nodes group (the constituent ratios were 95% and 90%, respectively) in the BALB/c nude mice, but there were four lymph nodes (the constituent ratios were 95% and 90%, respectively) in the BALB/c mice. There were significant difference between the BALB/c nude mice and the BALB/c mice. Mostly there were two lymph nodes of deep cervical lymph nodes both in the BALB/c nude mice and the BALB/c mice (the constituent ratios were 95% and 100%, respectively). There were no significant difference between the BALB/c nude mice and the BALB/c mice. We confirmed that the number of CD3-positive T lymphocytes in lymph nodes of the nude mice decreased greatly as compared with the BALB/c mice. Expressions of CD3 in T cells were 95% and 100% in the BALB/c nude mice and in the BALB/c mice, respectively. There were significant differences between the BALB/c nude mice and the BALB/c mice. Expressions of CD20 in B cells were 95% and 100% in the BALB/c nude mice and in the BALB/c mice, respectively. There was no significant difference between the BALB/c nude mice and BALB/c mice. Conclusion: The anatomical pictures of lymph node distribution in the nude mouse will be benefit to those who are interested. The anatomical features of the lymph node local higher density in neck of the nude mouse and lack of CD3-positive T lymphocytes would be useful for obtaining a better understanding of localized lymph node metastasis of oral transplant tumors.

Key words: Nude mice, Lymph nodes, Anatomy

CLC Number: 

  • R-332
[1] Pantelouris EM. Absence of thymus in a mouse mutant [J]. Nature, 1968, 217(5126): 370-371.
[2] Kurooka H, Segre JA, Hirano Y, et al. Rescue of the hairless phenotype in nude mice by transgenic insertion of the wild-type Hfh11 genomic locus [J]. Int Immunol, 1996, 8(6): 961-966.
[3] Cordier AC, Haumont SM. Development of thymus, parathyroids, and ultimo-branchial bodies in NMRI and nude mice [J]. Am J Anat, 1980, 157(3): 227-263.
[4] Rygaard J, Povlsen CO. Heterotransplantation of a human malignant tumour to “Nude” mice [J]. Acta Pathol Microbiol Scand, 1969, 77(4): 758-760.
[5] Kramer RH, Bensch KG, Wong J. Invasion of reconstituted basement membrane matrix by metastatic human tumor cells [J]. Cancer Res, 1986, 46(4 Pt 2): 1980-1989..
[6] Schor SL, Schor AM, Winn B, et al. The use of three-dimen-sional collagen gels for the study of tumour cell invasion in vitro : experimental parameters influencing cell migration into the gel matrix [J]. Int J Cancer, 1982, 29(1): 57-62.
[7] Horinaga M, Harsch KM, Fukuyama R, et al. Intravesical interleukin-12 gene therapy in an orthotopic bladder cancer model [J]. Urology, 2005, 66(2): 461-466.
[8] Kawashiri S, Kumagai S, Kojima K, et al. Development of a new invasion and metastasis model of human oral squamous cell carcinomas [J]. Eur J Cancer B Oral Oncol, 1995, 31B(4): 216-221.
[9] Matsui T, Ota T, Ueda Y, et al. Isolation of a highly metastatic cell line to lymph node in human oral squamous cell carcinoma by orthotopic implantation in nude mice [J]. Oral Oncol, 1998, 34(4): 253-256.
[10] Schmitz V, Tirado-Ledo L, Tiemann K, et al. Establishment of an orthotopic tumour model for hepatocellular carcinoma and non-invasive in vivo tumour imaging by high resolution ultrasound in mice [J]. J Hepatol, 2004, 40(5): 787-791.
[11] 杨安峰, 王平. 大鼠的解剖和组织[M]. 北京: 科学出版社, 1985.
[12] Fossum S. Differences between lymph node structure and function in normal and athymic rats [J]. Curr Top Pathol, 1990, 84 (Pt 1): 65-83.
[13] MacDonald HR, Lees RK, Sordat B, et al. Age-associated increase in expression of the T cell surface markers Thy-1, Lyt-1, and Lyt-2 in congenitally athymic (nu/nu) mice: analysis by flow microfluorometry [J]. J Immunol, 1981, 126(3): 865-870.
[14] Scheid MP, Goldstein G, Boyse E. Differentiation of T cells in nude mice [J]. Science, 1975, 190(19): 1211-1213.
[15] Ohman J, Mowjood R, Larsson L, et al. Presence of CD3-positive T-cells in oral premalignant leukoplakia indicates prevention of cancer transformation [J]. Anticancer Res, 2015, 35(1): 311-317.
[16] Dunn GP, Bruce AT, Ikeda H, et al. Cancer immunoediting: from immunosurveillance to tumor escape [J]. Nat Immunol, 2002, 3(11): 991-998.
[17] Sun R, Zhang JG, Guo CB. Establishment of cervical lymph node metastasis model of squamous cell carcinoma in the oral cavity in mice [J]. Chin Med J (Engl), 2008, 121(19): 1891-1895.
[18] Dinesman A, Haughey B, Gates GA, et al. Development of a new in vivo model for head and neck cancer [J]. Otolaryngol Head Neck Surg, 1990, 103(5 Pt 1): 766-774.
[19] Kawashiri S, Kojima K, Kumagai S, et al. Effects of chemotherapy on invasion and metastasis of oral cavity cancer in mice [J]. Head Neck, 2001, 23(9): 764-771.
[20] Simon C, Nemechek AJ, Boyd D, et al. An orthotopic floor-of-mouth cancer model allows quantification of tumor invasion [J]. Laryngoscope, 1998, 108(11 Pt 1): 1686-1691.
[21] Zhang X, Liu Y, Gilcrease MZ, et al. A lymph node metastatic mouse model reveals alterations of metastasis-related gene expression in metastatic human oral carcinoma sublines selected from a poorly metastatic parental cell line [J]. Cancer, 2002, 95(8): 1663-1672.
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