275 nm和310 nm紫外线对去卵巢骨质疏松大鼠骨代谢的影响

doi:10.19723/j.issn.1671-167X.2022.02.006

Abstract

Abstract:

Objective: To investigate the effect of 275 nm and 310 nm ultraviolet irradiation on ovariectomized rats’ bone metabolism. Methods: Twenty four 3-month-old female Sprague-Dawley (SD) rat were randomly divided into control group, sham operated group, 275 nm ultraviolet (UV) irradiation group and 310 nm UV irradiation group. Each group contained 6 rats. The rats in the two irradiation groups were treated with bilateral ovariectomy. The rats in sham operated group received sham operation (They were given the same back incision and a bit of par-ovarian fat were removed). Control group received no disposition. About 24 weeks after operation, all the rats received detailed bone mineral density (BMD) detection again. Detection regions include cervical vertebra, lumbar vertebra, proximal femur, mid femur and distal femur. Next, osteopenia rats in 275 nm irradiation group were UV irradiated 275 nm with fixed illumination intensity (15 μW/cm²) everyday for 16 weeks. The osteopenia rats in 310 nm irradiation group were UV irradiated 310 nm with fixed illumination intensity (15 μW/cm ²) everyday for 16 weeks. The backs of the rats were shaved regularly as irradiation area (6 cm×8 cm). After 16-week irradiation, all the rats’ BMD of cervical vertebra, lumbar vertebra, proximal femur, mid femur and distal femur were measured. At the end of the trial, all the rats’ blood specimens were obtained and serum 25(OH)D, procollagen type Ⅰ N-peptide (PINP) and osteocalcin (OC) were measured. Results: Compared with control group [(238.78±26.74) mg/cm³], the BMD of the whole body were significantly lower in 275 nm [(193.34±13.28) mg/cm³] and 310 nm [(191.19±18.48) mg/cm³] irradiation groups (P=0.002,P=0.001). There were no significant difference between sham operated group [(227.20±14.32) mg/cm³] and control group. After 16-week ultraviolet irradiation, the BMD of the whole body were significantly increased in 275 nm [(193.34±13.28) mg/cm³ vs. (221.68±25.52) mg/cm ³,P=0.005] and 310 nm groups [(191.19±18.48) mg/cm³ vs. (267.48±20.54) mg/cm ³,P<0.001] after corresponding irradiation. The BMD of the four body regions (lumbar vertebra, proximal femur, mid femur and distal femur) had significantly increased after irradiation in 275 nm irradiation group. For 310 nm irradiation group, the BMD in cervical vertebra, lumbar vertebra, proximal femur, mid femur and distal femur also had increased significantly after 310 nm ultraviolet irradiation. The concentration of serum 25(OH)D and OC was higher in 275 nm irradiation group than in control group [(46.78±5.59) μg/L vs. (21.32±6.65) μg/L, P=0.002;(2.05±0.53) U/L vs. (1.32±0.07) U/L,P=0.022]. Compared with the control, the concentration of serum 25(OH)D [(58.05±12.74) μg/L], OC [(2.04±0.53) U/L] and PINP [(176.16±24.18) U/L] was significantly higher (P<0.001, P=0.015, P=0.005) in 310 nm irradiation group. However, there were no significantly difference between sham operated group and the control. Conclusion: Both 275 nm and 310 nm ultraviolet could improve rats’ vitamin D synthesis. Both 275 nm and 310 nm ultraviolet could improve osteopenia rats’ bone condition. The irradiation of 310 nm might be more effective on bone condition improvement.

Key words: Ultraviolet, Ovariectomized Sprague-Dawley rats, Bone metabolism, Vitamin D

CLC Number:

R173.4

HE Wei,YANG Si-wen,CHEN Juan,ZHU Xiao-jun,CHEN Zhi-zhong,MA Wen-jun. Effects of 275 nm and 310 nm ultraviolet irradiation on bone metabolism in ovariectomized osteoporotic rats[J].Journal of Peking University (Health Sciences), 2022, 54(2): 236-243.

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References 26

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Items	BMD of whole body	Cervical vertebra	Lumbar vertebra	Proximal femur	Mid femur	Distal femur
Control	238.78±26.74	190.31±24.05	251.21±75.13	351.04±31.27	365.68±28.92	345.73±36.87
Sham operated	227.20±14.32	250.06±75.01	225.27±45.15	373.56±41.64	370.31±23.55	377.89±45.75
275 nm	193.34±13.28^*#	210.88±9.99	260.42±35.29	298.48±11.59^*#	317.88±15.59^*#	307.02±14.06^*#
310 nm	191.19±18.48^*#	197.69±34.42	300.98±23.66	297.32±14.62^*#	302.62±25.83^*#	301.33±14.04^*#
F value	8.09	2.38	2.56	12.42	12.57	9.07
P value	<0.001	0.099	0.083	<0.001	<0.001	<0.001

Items	BMD of whole body	Cervical vertebra	Lumbar vertebra	Proximal femur	Mid femur	Distal femur
Control	233.17±39.74	191.66±25.91	231.39±41.02	343.53±37.72	357.12±26.26	329.21±19.56
Sham operated	233.68±6.02	222.85±64.86	232.55±49.59	352.10±34.42	357.58±38.74	350.18±35.73
275 nm	221.68±25.52^★	224.62±48.72	289.51±27.61^★	323.69±45.79^★	358.44±48.86^★	353.33±44.63^★
310 nm	267.48±20.54^★	249.59±56.49^★	399.60±80.90^*#★	389.77±51.37^★	424.00±95.52^★	430.37±89.81^1★
F value	3.64	1.3	13.57	2.63	1.98	4.11
P value	0.027	0.301	<0.001	0.077	0.149	0.019

Items	25(OH)D/(μg/L)	PINP/(U/L)	OC/(U/L)
Control	21.32±6.65	129.41±2.14	1.32±0.07
Sham operated	20.42±5.76	130.12±10.17	1.32±0.08
275 nm	46.78±5.59^*#	157.07±13.80	2.05±0.53^*#
310 nm	58.05±12.74^*#	176.16±24.18^*#	2.04±0.53^*#
F value	19.6	7.68	7.06
P value	<0.001	0.001	0.002

Effects of 275 nm and 310 nm ultraviolet irradiation on bone metabolism in ovariectomized osteoporotic rats

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