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Journal of Peking University (Health Sciences) ›› 2024, Vol. 56 ›› Issue (5): 839-844. doi: 10.19723/j.issn.1671-167X.2024.05.014

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Flow cytometry analysis of normal range of natural killer cells and their subsets in peripheral blood of healthy Chinese adults

Jiayi TIAN, Yixue GUO, Xia ZHANG, Xiaolin SUN, Jing HE*()   

  1. Department of Rheumatology and Immunology, Peking University People's Hospital, Beijing 100044, China
  • Received:2021-06-20 Online:2024-10-18 Published:2024-10-16
  • Contact: Jing HE E-mail:hejing1105@126.com
  • Supported by:
    the Peking University People's Hospital Research and Development Funds(RS2020-01)

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Abstract:

Objective: To study the distribution characteristics of natural killer (NK) cells and their subsets in normal peripheral blood in China, and to explore their normal value and significance. Methods: In this study, peripheral blood was collected from 200 healthy adults. Their age range was 18-87 years. All the subjects were divided into 6 age groups: 18-30, 31-40, 41-50, 51-60, 61-70, and 71-87 years. With CD16, CD56, CD4, CD19, as surface markers, fluid cytology detection techniques were used to detect NK cells and the relative and absolute counts. SPSS 27.0 was used for systematic analysis of the data, and the measurement data were expressed as mean±standard deviations. A t test, variance analysis or rank sum test were performed to compare the differences between the age groups and the sex groups. The significance level was set at α=0.05, and P < 0.05 was considered statistically significant. Results: The range of NK B cells in the 200 healthy adult subjects was (0.46±0.24)×106/L, that of CD3-CD56+NK cells was (13.14±7.56)×106/L, that of CD56dimCD16+NK cells was (5.23±3.12)×106/L, that of CD56brightNK cells was (85.61±7.40)×106/L, and that of NK T cells was (4.16±3.34)×106/L. There were no statistically significant differences in CD3-CD56+NK cells and NK T cells with respect to age (P= 0.417, P=0.217). However, there was a decreasing trend in the number of NK B cells and CD56dimCD16+NK cells with increasing age (r=0.234, P < 0.001; r=0.099, P < 0.001), particularly after the age of 50. Conversely, CD56brightNK cells showed an increasing trend with age (r=0.143, P < 0.001). Conclusion: The detection of NK cells and their subsets has significant reference value for the diagnosis, treatment, and prognosis of autoimmune diseases, infectious diseases, and tumors. This study provides a preliminary reference range for clinical detection of NK cell subsets, but further research with a larger sample size and multi-center trials are needed to confirm these findings.

Key words: Natural killer cells, Natural killer cells and their subsets, Normal value

CLC Number: 

  • R593.2

Figure 1

Analysis of peripheral lymphocytes and natural killer cells and their subsets by flow cytometry NK, natural killer."

Figure 2

Differences in NK cells and their subsets between male and female NK, natural killer."

Figure 3

Differences of NK cells and their subsets in different age groups NK, natural killer."

Figure 4

Correlation of NK cells and their subsets with age NK, natural killer."

1 Yu J , Freud AG , Caligiuri MA . Location and cellular stages of natural killer cell development[J]. Trends Immunol, 2013, 34 (12): 573- 582.
2 Carrega P , Ferlazzo G . Natural killer cell distribution and trafficking in human tissues[J]. Front Immunol, 2012, 3, 347.
3 Zhu L , Karakizlis H , Weimer R , et al. Circulating NKG2A-NKG2D+ CD56dimCD16+ natural killer (NK) cells as mediators of functional immunosurveillance in kidney transplant recipients[J]. Ann Transplant, 2020, 25, e925162.
4 Marras F , Casabianca A , Bozzano F , et al. Control of the HIV-1 DNA reservoir is associated in vivo and in vitro with NKp46/NKp30 (CD335 CD337) inducibility and interferon gamma production by transcriptionally unique NK cells[J]. J Virol, 2017, 91 (23): e00647.
5 Cichocki F , Grzywacz B , Miller JS . Human NK cell development: One road or many?[J]. Front Immunol, 2019, 10, 2078.
6 Freud AG , Mundy-Bosse BL , Yu J , et al. The broad spectrum of human natural killer cell diversity[J]. Immunity, 2017, 47 (5): 820- 833.
7 Melzer S , Zachariae S , Bocsi J , et al. Reference intervals for leukocyte subsets in adults: Results from a population-based study using 10-color flow cytometry[J]. Cytometry B Clin Cytom, 2015, 88 (4): 270- 281.
8 Shu SA , Wang J , Tao MH , et al. Gene therapy for autoimmune disease[J]. Clin Rev Allergy Immunol, 2015, 49 (2): 163- 176.
9 Choi J , Lee SJ , Lee YA , et al. Reference values for peripheral blood lymphocyte subsets in a healthy korean population[J]. Immune Netw, 2014, 14 (6): 289- 295.
10 Yawata N , Selva KJ , Liu YC , et al. Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection[J]. Mucosal Immunol, 2016, 9 (1): 159- 170.
11 Tahrali I , Kucuksezer UC , Akdeniz N , et al. CD3-CD56+ NK cells display an inflammatory profile in RR-MS patients[J]. Immunol Lett, 2019, 216, 63- 69.
12 Bendelac A , Savage PB , Teyton L . The biology of NK T cells[J]. Annu Rev Immunol, 2007, 25, 297- 336.
13 McCarthy C , Shepherd D , Fleire S , et al. The length of lipids bound to human CD1d molecules modulates the affinity of NK T cell TCR and the threshold of NK T cell activation[J]. J Exp Med, 2007, 204 (5): 1131- 1144.
14 Simoni Y , Diana J , Ghazarian L , et al. Therapeutic manipulation of natural killer (NK) T cells in autoimmunity: Are we close to reality?[J]. Clin Exp Immunol, 2013, 171 (1): 8- 19.
15 Li M , Xiong Y , Li M , et al. Depletion but activation of CD56(dim)CD16(+)NK cells in acute infection with severe fever with thrombocytopenia syndrome virus[J]. Virol Sin, 2020, 35 (5): 588- 598.
16 Gayoso I , Sanchez-Correa B , Campos C , et al. Immunosene-scence of human natural killer cells[J]. J Innate Immun, 2011, 3 (4): 337- 343.
17 Solana R , Tarazona R , Gayoso I , et al. Innate immunosene-scence: Effect of aging on cells and receptors of the innate immune system in humans[J]. Semin Immunol, 2012, 24 (5): 331- 341.
18 Solana R , Pawelec G , Tarazona R . Aging and innate immunity[J]. Immunity, 2006, 24 (5): 491- 494.
19 Cunha CF , Ferraz-Nogueira R , Costa VFA , et al. Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+CD56+NK T cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles[J]. PLoS One, 2020, 15 (3): e0229400.
20 Myers JA , Miller JS . Exploring the NK cell platform for cancer immunotherapy[J]. Nat Rev Clin Oncol, 2021, 18 (2): 85- 100.
21 Terrén I , Orrantia A , Vitallé J , et al. NK Cell metabolism and tumor microenvironment[J]. Frontiers in immunology, 2019, 10, 2278.
22 Zhang C , Liu Y . Targeting NK Cell Checkpoint receptors or molecules for cancer immunotherapy[J]. Front Immunol, 2020, 11, 1295.
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