Journal of Peking University(Health Sciences) ›› 2018, Vol. 50 ›› Issue (2): 203-206. doi: 10.3969/j.issn.1671-167X.2018.02.001

• Article •     Next Articles

Role of aquaporin-4 in the change of interstitial fluid drainage in Alzheimer’s disease

LIU E1, ZHANG Yi-xuan1, SUN Lin-lin1,TENG Ze2, WANG Ai-bo2, HAN Hong-bin2, YAN Jun-hao1△   

  1. (1. Department of Anatomy and Embryology, School of Basic Medical Sciences, Peking University, Beijing 100191, China; 2. Beijing Key Lab of Magnetic Resonance Imaging Device and Technique, Beijing 100191, China)
  • Online:2018-04-18 Published:2018-04-18
  • Contact: YAN Jun-hao E-mail:yjh@bjmu.edu.cn
  • Supported by:
    助Supported by the National Natural Science Foundation of China (31471028) and Peking University Seed Fund for Medicine-Chemistry Interdisciplinary Research Project(BMU2018MC001)

Abstract: Objective: To observe the characteristics of the interstitial fluid (ISF) drainage in the Alzheimer’s disease (AD) rats through magnetic resonance imaging (MRI) tracer gadolinium-diethylene triamine pentacetic acid (Gd-DTPA)spread in the brain extracellular space (ECS) and to discuss the role of aquaporin-4 (Aqp4) in the AD. Methods: Wild type SD rats (300-350 g) and Aqp4 gene knock out (Aqp4-/-) SD rats (300-350g) were divided into Sham group, AD group, Aqp4-/-Sham group and Aqp4-/--AD group. Sham group and Aqp4-/--Sham group were injected with saline by intraperitoneal each day for 6 weeks, and the AD group and Aqp4-/--AD group were injected with D-galactose by intraperitoneal each day for 6 weeks. MRI tracer Gd-DTPA (10 mmol/L, 2 μL) was injected into the hippocampus of the rats. MRI scan was performed at the end of 0.5 h, 1.5 h, 1 h, 2 h, and 3 h to observe the dynamic distribution of the Gd-DTPA in the hippocampus and the diffusion rate D*, clearance rate k′ and half-life t1/2 measured. Results: The diffusion rate D* in Sham group was (2.66±0.36)×10-6 mm2/s, the diffusion rate D* in AD group was (2.72±0.62)×10-6 mm2/s, the diffusion rate D* in Aqp4-/--Sham group was (2.75±0.47)×10-6  mm2/s, the diffusion rate D* in Aqp4-/--AD group was (2.802±0.55)×10-6  mm2/s, and there was no statistically significant difference in the four groups (One-Way ANOVA, P>0.05).The clearance rate k′ in Sham group was (4.57±0.14)×10-4/s, the clearance rate k′ in AD group was (3.68±0.22)×10-4/s, the clea-rance rate k′ in Aqp4-/-Sham group was (3.17±0.16)×10-4/s, the clearance rate k′ in Aqp4-/--AD group was (2.59±0.19)×10-4/s, and there was significant difference in the four groups (One-Way ANOVA, P<0.05). The halflife t1/2 in Sham group was (0.67±0.12) h, the halflife t1/2 in AD group was (0.88±0.08) h, the half-life t1/2 in Aqp4-/--Sham group was (1.12±0.15) h, the half-life t1/2 in Aqp4-/--AD group was (1.58±0.11) h, and there was significance difference in the four groups(oneway ANOVA,P<0.05). Conclusion: The ISF drainage is slow after AD and the loss of Aqp4 in the AD makes the ISF drainage obviously slow down, Aqp4 plays an important role in AD to remove the metabolism of waste out of the brain.

Key words: Aquaporin-4, Alzheimer’s disease, Interstitial fluid, Magnetic resonance imaging

CLC Number: 

  • R445
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