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北京大学学报（医学版） ›› 2018, Vol. 50 ›› Issue (6): 981-985. doi: 10.19723/j.issn.1671-167X.2018.06.007

• 论著 • 上一篇    下一篇

血清基质金属蛋白酶3水平与早期类风湿关节炎病情评估

刘梦珂1,2,3,王露辰1,2,3,胡凡磊1,2,△()   

  1. 1. 北京大学人民医院风湿免疫科,风湿病机制及免疫诊断北京市重点实验室, 北京 100044
    2. 北京大学药学院天然药物及仿生药物国家重点实验室, 北京 100191
    3. 南昌大学玛丽女王学院, 南昌 330000
  • 收稿日期:2018-07-23 出版日期:2018-12-18 发布日期:2018-12-18
  • 通讯作者: 胡凡磊 E-mail:fanleihu@bjmu.edu.cn
  • 基金资助:
    国家自然科学基金(81671604);国家自然科学基金(81302554);国家自然科学基金(31530020);国家自然科学基金(81501396);国家自然科学基金(81871281);北京市科技新星计划(Z181100006218044);北京大学临床+X青年项目(PKU2018LCXQ003)

Value of serum matrix metalloproteinase 3 in the assessment of early rheumatoid arthritis

Meng-ke LIU1,2,3,Lu-chen WANG1,2,3,Fan-lei HU1,2,△()   

  1. 1. Department of Rheumatology and Immunology, Peking University People’s Hospital & Beijing Key Laboratory for Rheumatism Mechanism and Immune Diagnosis (BZ0135) Beijing 100044, China
    2. State Key Laboratory of Natural and Biomimetic Drugs,Peking University School of Pharmaceutical Sciences,Beijing 100191, China
    3. Nanchang University Queen Mary School, Nanchang 330000, China
  • Received:2018-07-23 Online:2018-12-18 Published:2018-12-18
  • Contact: Fan-lei HU E-mail:fanleihu@bjmu.edu.cn
  • Supported by:
    Supported by the National Natural Science Foundation of China(81671604);Supported by the National Natural Science Foundation of China(81302554);Supported by the National Natural Science Foundation of China(31530020);Supported by the National Natural Science Foundation of China(81501396);Supported by the National Natural Science Foundation of China(81871281);the Beijing Nova Program(Z181100006218044);Peking University Clinical Medcine + X Project PKU2018LCXQ003(PKU2018LCXQ003)

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摘要：

目的: 研究血清基质金属蛋白酶3(matrix metalloproteinase 3,MMP3)在C-反应蛋白(C-reaction protein,CRP)和/或红细胞沉降率(erythrocyte sedimentation rate,ESR)正常的早期类风湿关节炎(early rheumatoid arthritis,ERA)患者中的表达及在病情评估中的意义。方法: 选择北京大学人民医院2011年至2015年确诊的早期RA患者133例,骨关节炎(osteoarthritis,OA)患者25例,健康对照组60例,其中早期RA患者根据CRP、ESR水平分为4组,CRP升高ESR升高组88例,CRP正常ESR正常组15例,CRP正常ESR升高组17例,CRP升高ESR正常组13例,回顾患者临床资料,并采用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)法测定患者及健康对照血清中MMP3水平。结果: CRP正常和/或 ESR正常患者血清MMP3水平[(72.89±6.34) μg/L]明显高于OA组MMP3[(42.87±4.14) μg/L](P=0.002)和健康对照组MMP3水平[(31.62±2.88) μg/L](P<0.001), 其中,CRP正常ESR正常患者血清MMP3水平[(47.04±9.64) μg/L]高于健康对照组,差异具有统计学意义(P<0.05), CRP升高ESR正常患者血清MMP3水平[(94.18±9.11) μg/L]和CRP正常ESR升高患者血清MMP3水平[(79.42±10.60) μg/L]均高于OA组和健康对照组,差异有统计学意义(P<0.05)。在CRP正常和/或ESR正常的早期RA患者中,血清MMP3水平与CRP水平呈正相关(r=0.336,P=0.024)。CRP正常和/或 ESR正常患者MMP3阳性率为44.44%,高于CRP阳性率(28.89%)和ESR阳性率(37.78%), 其中CRP正常ESR升高组阳性率52.94%,CRP升高ESR正常组阳性率53.85%。结论: 血清MMP3水平的测定对于CRP和/或ESR正常的病程小于2年的早期类风湿关节炎患者具有辅助评估价值。

关键词: 基质金属蛋白酶3, 早期类风湿关节炎, C-反应蛋白, 红细胞沉降率, 评估价值

Abstract:

Objective: To investigate the expression level of serum matrix metalloproteinase 3 (MMP3) in early rheumatoid arthritis (ERA) patients with normal C-reaction protein (CRP) or erythrocyte sedimentation rate (ESR), and the significance in disease assessment.Methods:In the study, 133 cases of early RA patients, 25 osteoarthritis (OA) patients and 60 healthy controls in Peking University People’s Hospital from 2011 to 2015 were included. The RA patients were further divided into 4 groups according to levels of CRP and ESR: 88 patients with increased CRP and increased ESR, 15 patients with normal CRP and normal ESR, 17 patients with normal CRP but increased ESR, and 13 patients with increased CRP but normal ESR. All the clinical information of the patients was collected, and the serum MMP3 levels of both patients and healthy controls were detected by enzyme-linked immune sorbent assay (ELISA).Results:The serum MMP3 level of RA patients with normal CRP and/or normal ESR [(72.89±6.34) μg/L] was obviously higher than that of OA patients [(42.87±4.14) μg/L](P=0.002) and healthy controls [(31.62±2.88) μg/L](P<0.001). The serum MMP3 levels of the patients with normal CRP and normal ESR[(47.04±9.64) μg/L] were higher than those of the healthy controls, and there was statistical significance between the two groups (P<0.05). The serum MMP3 levels of the patients with increased CRP but normal ESR [(94.18±9.11) μg/L] and the patients with normal CRP but increased ESR [(79.42±10.60) μg/L] were both higher than those of the OA patients and healthy controls, and there was obvious statistical difference (P<0.05). In the early RA patients with normal CRP and/or normal ESR, the serum MMP3 level was positively correlated with the CRP level (r=0.336,P=0.024). The positive rate of MMP3 in the patients with normal CRP and/or normal ESR was 44.44%, higher than the positive rate of CRP (28.89%) and the positive rate of ESR (37.78%).In these early RA patients, the positive rate was 52.94% in the patients with normal CRP but increased ESR and 53.85% in the patients with increased CRP but normal ESR.Conclusion:The detection of the serum MMP3 level was significant in the assessment of early RA patients within 2-year duration who had normal CRP or ESR value.

Key words: Matrix metalloproteinase 3, Early rheumatoid arthritis, C-reaction protein, Erythrocyte sedimentation rate, Assessment value

中图分类号: 

  • R593.22

表1

CRP正常和/或 ESR正常早期RA患者CRP、ESR、MMP3阳性率比较"

Items Total cases Positive cases Positive rate/%
MMP3 45 20 44.44
CRP 45 13 28.89
ESR 45 17 37.78

表2

CRP正常或ESR正常情况下与MMP3阳性率比较"

Items Normal CRP Normal ESR
ESR MMP3 CRP MMP3
Total cases 32 32 28 28
Positive cases 17 13 13 11
Positive rate/% 53.13 40.63 46.43 39.29

表3

病程6个月内极早期RA患者CRP、ESR、MMP3阳性率比较"

Items Total cases Positive cases Positive rate/%
MMP3 55 44 80.00
CRP 55 42 76.36
ESR 55 45 81.82

表4

各组RA患者MMP3水平阳性率"

Group Total cases MMP3 positive Positive
rate/%
Normal CRP and/or normal ESR 45 20 44.44
Normal CRP increased ESR 17 9 52.94
Increased CRP normal ESR 13 7 53.85
Normal CRP normal ESR 15 4 26.67

图1

不同CRP、ESR分组的早期RA患者与OA、健康对照组MMP3水平比较"

表5

MMP3水平与临床指标相关性"

Items Increased CRP increased ESR Normal CRP and/or normal ESR
r P r P
CRP 0.125 0.254 0.336 0.024*
ESR 0.318 0.003# 0.069 0.651
IgA -0.100 0.376 0.099 0.531
IgG 0.237 0.035* -0.112 0.478
IgM 0.107 0.346 -0.259 0.097
RF -0.245 0.053 -0.122 0.435
anti-CCP -0.293 0.012* -0.008 0.964
Tender joint count 0.149 0.181 -0.074 0.628
Swollen joint count 0.153 0.167 -0.023 0.883
DAS28 0.248 0.025* -0.017 0.916

表6

不同CRP、ESR水平分组的早期RA患者IgA、IgG、IgM水平比较"

Groups IgA/(g/L) IgG/(g/L) IgM/(g/L)
Increased CRP increased ESR 3.28±1.37 14.98±4.77 1.25±0.72
Increased CRP normal ESR 2.51±1.16 14.21±6.54 0.72±0.26*
Normal CRP increased ESR 3.80±1.67 14.99±4.08 1.43±0.77
Normal CRP normal ESR 2.44±0.87* 13.27±2.49 0.98±0.39
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