目的：建立和优化小鼠脑组织中2,4-二羟基二苯甲酮（2,4-dihydroxybenzophenone，BP-1）的测定方法。方法：采用正交试验设计优化样品预处理条件，Waters Symmetry^®C18（4.6 mm×250 mm，5 μm）色谱柱分离，等度洗脱，流动相为含3%（体积分数）乙酸的甲醇-水（体积比为3∶1），pH为3.40，流速为1.0 mL/min，紫外检测器检测，检测波长为290 nm，高效液相色谱法测定小鼠脑组织中BP-1，保留时间定性，以内标法定量。结果：在优化的实验条件下，BP-1在0.2~10.0 mg/L浓度范围内线性关系良好，相关系数为0.999 8；BP-1的加标回收率为96.8%~104.5%；日内和日间精密度分别为3.5%~5.7%和4.5%~6.4%；低、中、高3种浓度BP-1的提取回收率分别为90.5%、89.5%和97.7%；低、中、高3种浓度BP-1的基质效应分别为102.9%、102.7%和90.9%。结论：该方法简便、准确，适用于小鼠脑组织中BP-1的测定。

Objective：To optimize and establish the experimental methods for the determination of 2,4-dihydroxybenzophenone (BP-1) in mouse brain. Methods: BP-1 was determined by high performance liquid chromatography (HPLC) and separated by Waters Symmetry^® C18 (4.6 mm×250 mm, 5 μm) using isocratic elution, and the sample preparation conditions were optimized by orthogonal experiment design. The mobile phase was methanolwater (volume ratio 3∶1) containing 3% (volume fraction) acetic acid (pH 3.40) at a flow rate of 1.0 mL/min, and ultraviolet (UV) detection wavelength was set at 290 nm. Retention time was used for qualitative analysis and internal standard method for quantitative analysis. Results: Under the optimized experimental conditions, the calibration curve was linear with a correlation coefficient of 0.999 8 over the concentration range of 0.2-10.0 mg/L. The recoveries of BP-1 were between 96.8% and 104.5%. The intra-day and inter-day precision of BP-1 were 3.5%-5.7% and 4.5%-6.4%, respectively. The extraction recoveries of BP-1 at three concentrations (0.5, 2.0, 8.0 mg/L) in the mouse brain were 90.5%, 89.5%, and 97.7%, and the matrix effect of BP-1 at these three concentrations were 102.9%, 102.7%, and 90.9%, respectively. Conclusion:The method is simple, accurate, and suitable for determination of the contents of BP-1 in mouse brain.