目的：初步研究纳米羟基磷灰石对人脂肪间充质干细胞（human adiposederived mesenchymal stem cells，hASCs）共混物三维生物打印体细胞增殖和成骨分化的影响。方法：以P5代hASCs作为种子细胞，将10 g/L纳米羟基磷灰石加入细胞海藻酸钠明胶共混物（20 g/L海藻酸钠，80 g/L明胶，细胞密度约为1×106个/mL）混合后进行三维生物打印，作为实验组；另将细胞海藻酸钠明胶共混物直接进行三维生物打印，作为对照组。在打印后的既定时间点上，对实验组和对照组分别进行显微镜观察、CCK8检测、Western blot检测和PCR检测，分析打印体细胞增殖能力和成骨分化能力。结果：显微镜观察和CCK8检测结果显示，实验组和对照组打印体内细胞在打印完成后24 h和7 d均呈现良好的增殖能力。Western blot结果显示，在打印完成后14 d，实验组和对照组的Runt相关转录因子（Runtrelated transcription factor 2，RUNX2）蛋白水平差异无统计学意义。PCR结果显示，在打印完成后14 d，实验组的成骨相关基因RUNX2、osterix（OSX）和骨钙素（osteocalcin，OCN）表达明显高于对照组。结论：纳米羟基磷灰石加入后，打印体内细胞仍具有良好的增殖性，且打印体的体外成骨分化能力得到了增强。

Objective：To study the effect of nano hydroxyapatite on human adipose-derived mesenchymal stem cells(hASCs) mixture 3D bio-printing for cells’ proliferation and osteogenesis. Methods: P5 hASCs were used as seed cells, 10 g/L nano hydroxyapatite was added into the cell-sodium alginate-gelatin mixture (concentration: 20 g/L sodium alginate, 80 g/L gelatin; cell density: 1×106/mL), then the mixture was printed by 3D bio-printer as the experimental group. And the cell-sodium alginate-gelatin mixture without nano hydroxyapatite was printed as the control group. Respectively, both the experimental and control groups were detected by microscope, CCK-8, Western blot and PCR at certain time pointsafter being printed, whose cells’ proliferation and osteogenic differentiation were analyzed. Results:  The microscopic observation and CCK-8 results showed that the cells of the experimental group and the control group both had a good proliferation 24 h and 7 d after being printed. The Western blot results showed that 14 d after printing, the expression of Runtrelated transcription factor 2 (RUNX2) had no statistical difference between the experimental group and control group. The PCR results showed that 14 d after printing, the expression of osteogenesis-related genes (RUNX2, osterix, and osteocalcin) was significantly higher in the experimental group than in the control group. Conclusion:  Nano hydroxyapatite can increase osteogenic differentiation of the hASCs mixture after bio-printing, in which the cells still have a good proliferation.