目的：观察咬合干扰后不同时间大鼠咬肌能量代谢产物腺嘌呤核苷三磷酸（adenosine triphosphate，ATP）、腺嘌呤核苷二磷酸（adenosine diphosphate，ADP）、次黄嘌呤核苷酸（inosine monophosphate，IMP）、磷酸肌酸、肌酸、乳酸及pH水平的变化，分析咬合干扰对咀嚼肌能量代谢的影响。方法：选用雄性Sprague-Dawley大鼠（220~250 g）50只，随机分为实验组（40只）和对照组（10只），实验组于右上第一磨牙粘固0.4 mm厚金属冠建立咬合干扰，并分别维持3、7、10、14 d（每个时间点各10只）， 对照组不施加咬合干扰。各组大鼠全麻下取双侧咬肌组织，其中5只大鼠样本加入0.4 mol/L高氯酸（10 mL/g）充分匀浆，离心、过滤后采用高效液相色谱分析ATP、ADP、IMP、磷酸肌酸、肌酸及乳酸含量，另外5只大鼠样本加入含5 mmol/L碘醋酸钠的匀浆液（10 mL/g），充分匀浆后在37 ℃恒温水浴环境中利用pH计测试pH值。结果：与对照组相比，大鼠双侧咬肌ATP含量在咬合干扰3 d ［右侧：（5.36±0.13） μmol/g，左侧：（5.77±0.25） μmol/g］ 升高（P<0.05），7、10和14 d没有显著改变；大鼠双侧咬肌IMP［右侧：（0.21±0.03） μmol/g，左侧：（0.19±0.03） μmol/g］、肌酸［右侧：（24.76±2.94） μmol/g，左侧：（27.75±2.23） μmol/g］含量在咬合干扰7 d升高（P<0.05），3、10和14 d没有显著改变；大鼠双侧咬肌磷酸肌酸含量在咬合干扰7、10和14 d降低［右侧分别为：（10.70±0.71） μmol/g、（11.57±0.52） μmol/g、（10.74±1.39） μmol/g，左侧分别为：（10.05±0.57） μmol/g、（10.75±1.12） μmol/g、（10.61±1.15） μmol/g， P<0.05］，3 d没有显著改变；大鼠双侧咬肌ADP、乳酸含量及pH水平在咬合干扰后各时间点均没有显著改变（P>0.05）。结论：咬合干扰导致大鼠咀嚼肌能量代谢产物含量改变，可能与咬合干扰诱发咀嚼肌疼痛、功能紊乱、肌纤维构筑改变等病理过程相关。

Objective： To investigate the effect of occlusal interference on the energy metabolism of masticatory muscle by studying the changes of adenosine triphosphate (ATP), adenosine diphosphate (ADP), inosine monophosphate (IMP), phosphocreatine, creatine, lactate and pH level in masseter muscles of rats after occlusal interference. Methods: Fifty male Sprague-Dawley rats were randomly assigned into experimental group (n=40) and control group (n=10). In experimental group, 0.4 mm thick metal crown was cemented to the upper right first molar of the rat, and maintained for 3, 7, 10, 14 d separately (n=10 for each time point). No occlusal interference was applied for control group. Bilateral masseter muscles of all the rats were acquired under general anesthesia. The samples of 5 rats in each group were fully homogenized with 0.4 mol/L perchlorate (10 mL/g). The homogenates were centrifuged, filtered and analyzed for ATP, ADP, IMP, phosphocreatine, creatine and lactate content by high performance liquid chromatography. The other samples in each group were mixed with homogenates containing 5 mmol/L sodium iodoacetate (10 mL/g), then homogenized and measured for pH value by pH meter in thermostatic water bathunder 37 degrees centigrade. Results: Compared with control group, ATP content in bilateral masseter of the rats increased  3 d after occlusal interference ［right side：(5.36±0.13) μmol/g，left side：(5.77±0.25) μmol/g］ (P<0.05), and back to normal on 7, 10 and 14 d- There was an increase in IMP ［right side：(0.21±0.03) μmol/g，left side：(0.19±0.03) μmol/g］and creatine content ［right side：(24.76±2.94) μmol/g，left side：(27.75±2.23) μmol/g］in bilateral masseter of the rats  7 d after occlusal interference (P<0.05) and no difference was detected on 3, 10, and 14 . Phosphocreatine content in bilateral masseter started to decline  7 d after occlusal interference and maintained the low level on 10 and 14 d ［right side：(10.70±0.71) μmol/g, (11.57±0.52) μmol/g, (10.74±1.39) μmol/g, left side：(10.05±0.57) μmol/g, (10.75±1.12)μmol/g, (10.61±1.15) μmol/g］(P<0.05). No change of ADP, lactate or pH level in bilateral muscles of the rats after occlusal interference was observed (P>0.05).  Conclusion: Occlusal interference influences the content of energy metabolites in masticatory muscle of rats, which may be related to the pathological process of masticatory muscles induced by occlusal interference, such as muscle pain, dysfunction and altered fiber architecture.