目的： 评估高糖对肾组织干细胞（kidney stem cells，KSC）条件培养液修复缺氧损伤肾小管上皮细胞（renal tubular epithelium cells，RTEC）作用的影响。方法： 分离肾乳头处的KSC，分别用正常糖浓度（简称“正糖”，5.6 mmol/L）和高糖（30 mmol/L）培养基对KSC进行预处理后制备KSC条件培养液。建立大鼠RTEC缺氧/复氧模型，比较高糖与正糖刺激后KSC条件培养液对缺氧/复氧RTEC修复作用的差异。结果： （1）缺氧4 h/复氧2 h为RTEC缺氧/复氧模型的最佳时间。（2）缺氧后，RTEC早期凋亡率和晚期凋亡率均升高，采用KSC条件培养液干预12 h和24 h后，与缺氧/复氧对照组相比，正糖缺氧/复氧组和高糖缺氧/复氧组RTEC的凋亡率均明显降低（P<0.01）。正糖组和高糖组比较，干预24 h后，正糖组RTEC的总体凋亡率显著低于高糖组（P=0.02）。（3）缺氧后，RTEC上清液的乳酸脱氢酶（lactic dehydrogenase，LDH）和丙二醛（malondialdehyde，MDA）水平明显升高（P<0.01），超氧化物歧化酶（superoxide dismutase，SOD）水平明显降低（P<0.01）。采用KSC条件培养液干预12 h和24 h后，与缺氧/复氧对照组相比，正糖缺氧/复氧组和高糖缺氧/复氧组的LDH和MDA水平均显著降低（P<0.01），SOD水平均显著升高（P<0.01）。正糖组和高糖组比较，高糖组的LDH和MDA水平要显著高于正糖组（P<0.05），SOD水平要显著低于正糖组（P<0.01）。结论： KSC条件培养液对缺氧损伤的RTEC有修复作用，这种作用主要是通过减少氧化应激、抑制细胞凋亡来实现的，而经高糖预处理的KSC条件培养液的抗氧化应激、抗凋亡作用减弱。

Objective： To evaluate the impacts of high glucose on the repair function of kidney stem cells (KSC) conditional medium to the hypoxia-injured renal tubular epithelium cells (RTEC). Me-thods: KSC were isolated from the renal papilla in 4-week-Sprague-Dawley rats. The KSC were pretreated in media with high glucose (30 mmol/L) or with normal glucose (5.6 mmol/L), respectively. The supernatants of the pre-treated KSC were collected as the conditional media. The hypoxia/reoxygenation (H/R) model of rat RTEC was established using the NRK-52E cell line. The effects of KSC conditional media on the H/R RTEC were investigated. Results: (1) The best H/R model of RTEC was established using hypoxia for 4 h and reoxygenation 2 h. (2) After hypoxia, the early and late cell apoptosis rates of the H/R RTEC were increased. The H/R RTEC were co-cultured with KSC conditional media for 12 h and 24 h, respectively. The H/R RTEC were co-cultured with DMEM/F12 as a control group. The cell apoptosis rate of H/R RTEC was lower after co-cultured with KSC conditional media (P<0.01), and the cell apoptosis rate of H/R RTEC in high glucose group was much higher than that in normal glucose group after co-cultured 24 h (P=0.02). (3) After hypoxia, the lactic dehydrogenase (LDH) and malondialdehyde (MDA) levels of the H/R RTEC supernatant were increased, and the superoxide dismutase (SOD) level decreased. The LDH and MDA levels were lower and the SOD level was higher after co-cultured with KSC conditional media for 12 h and 24 h, respectively (P<0.01). The LDH and MDA levels of H/R RTEC supernatant were much higher in the high glucose group than in the normal glucose group (P<0.05), and the SOD level of H/R RTEC supernatant was much lower in the high glucose group than in the normal glucose group (P<0.01). Conclusion: KSC conditional media could repair the H/R injury of RTEC. The effects were mainly by inhibiting cell apoptosis, and reducing oxidative stress; the anti-cell apoptosis ability and the anti-oxidative stress capacity of the conditional medium were reduced after KSC were pre-treated with high glucose.