目的：构建并改进前列腺癌（prostate cancer，PCa）体内股骨及脊柱转移动物模型，为不同部位的PCa骨转移的诊治及骨应力改变的研究提供工具。方法: 将不同浓度的PCa细胞(RM-1)注射到小鼠(C57BL/6)股骨骨髓腔和腰椎内，观察记录小鼠活动变化、成瘤情况，测定瘤体大小及记录存活时间。取模型的双侧股骨组织，进行影像学检查后测算灰度值(骨密度)， 并行组织病理学检查，确定建模情况及成瘤类型。结果:各细胞浓度梯度小鼠均成瘤，各股骨转移模型的肿瘤发生时间、生长速度同组间无差别（P>0.05），病理学检测结果证实所有股骨骨髓腔和腰椎内PCa骨转移模型构建成功，且较低RM-1细胞浓度构建的PCa骨转移动物模型生存时间较前期构建的动物模型明显延长（约1倍，至少2周）。结论: 成功用同一PCa细胞株构建了不同部位（四肢骨和中轴骨）的PCa骨转移体内动物模型，经股骨骨髓腔穿刺注射肿瘤细胞建立PCa骨转移动物模型成瘤率高，肿瘤生长迅速，且可稳定复制，为进一步PCa骨转移的骨应力改变及防治的研究提供平台。

Objective： To provide an important tool for the study of diagnose and treatment of prostate cancer (PCa) osseous metastasis and change of bone stress force on prostate cancer (PCa) osseous metastasis and a platform, which is more congruous to clinical process, for prevention and cure of neoplastic bone metastases, and to carry out the construction and improvement of animal models of PCa with different positional osseous metastasis in vivo. Methods: Different gradient concentrations of RM-1 cells were inoculated into the cavity of left femoral bone or lumbar vertebra of mice (C57BL/6) respectively. The change of mouse  activity, tumor formation, tumor size and survival time were observed respectively. And the femur tissue and spinal tissue were obtained from the mice after death. The gray value of iconography were measured by imageological examination of femur tissue, and the final histopathological examination were taken to determine the tumor type in both femur and spinal tissue. Results: The tumor growth could be touched at the puncture site in all the mice after inoculated for 7 days. There were no obvious differences in the time of tumorigenesis, the rate of tumor growth and tumor size among the mice in the same group (P>0.05). As the result, the construction femoral bone and lumbar vertebra metastatic models of PCa had been confirmed by iconography and pathology detection. At the same time, the survival time of the mice inoculated with low concentrations of PCa cells was obviously longer than that of high concentrations of PCa cells ( at least 2 weeks longer).   Conclusion: The animal models with different positional osseous metastasis (limbs and axial skeleton) of PCa using the same PCa cells (RM-1) had been first constructed successfully in our study. At the same time, a high success rate of construction of PCa animal model with bone metastasis was obtained by femoral bone marrow cavity injection of PCa cells. The rate of tumor growth was rapid, animal survival time was appropriate, and the PCa animal model with bone metastasis can be stably reproduced by our method. These animal models can be used to explore the pathogenesis of different positional PCa bone metastasis and provide a new platform, which were more congruous to clinical process, for prevention and cure of neoplastic bone metastases.