目的：应用细胞动态可视化系统观察并分析正常鼠和精神分裂症模型鼠腹腔中的中性粒细胞趋化运动能力及其差异。方法：18只健康昆明小鼠随机分为对照组（6只）、0.3 mg/kg 5-甲基二氢二苯并环庚烯亚氨马来酸（dizocilpine maleate,MK-801）处理组（6只）、0.6 mg/kg MK801处理组（6只），分别提取中性粒细胞，在显微镜下观察形态并计数。将每组细胞分成两份进行趋化实验，分别为趋化剂处理组和趋化剂未处理组，即对照组 1、0.3 mg/kg MK-801处理组1、0.6 mg/kg MK-801处理组 1和对照组 2、0.3 mg/kg MK-801处理组 2、0.6 mg/kg MK-801处理组 2。使用NIS-Elements软件记录细胞动态迁移过程。使用TAXIScan Analyzer 2软件每组细胞随机选取30个细胞（即n=30）分析细胞迁移的轨迹、速度和距离，并采用配对检验和单因素方差分析进行统计学分析。结果：对照组、0.3 mg/kg MK-801处理组、0.6 mg/kg MK-801处理组的中性粒细胞的数量分别是（1.00±0.03）×104/mL、（0.05±0.02）×104/mL、（0.32±0.01）×104/mL，差异均有统计学意义（P<0.05）。在趋化剂的作用下，对照组1、0.3 mg/kg MK-801处理组1、0.6 mg/kg MK-801处理组1的中性粒细胞定向迁移能力分别是（0.85±0.11） radian、（1.00±0.11） radian、（0.96±0.10） radian，差异均有统计学意义（P<0.05）；中性粒细胞迁移速度分别是（0.09±0.02） μm/s、（0.12±0.01） μm/s、（0.14±0.01） μm/s，差异均有统计学意义（P<0.05）；中性粒细胞迁移距离分别是（94.26±0.02） μm、（134.61±0.01） μm、（156.19±0.01） μm，差异均有统计学意义（P<0.05）。结论：与对照组腹腔中的中性粒细胞比较，精神分裂症模型鼠腹腔中的中性粒细胞具有更强的趋化运动能力。

Objective： To compare the differences of neutrophils chemotaxis ability in  peritoneal cavity between normal rats and schizopherenic rats with cell dynamic visualization system. Methods: In the study,18 healthy Kunming rats were randomly divided into 3 groups which were control group(n=6), 0.3 mg/kg MK801 treatment group(n=6）, 0.6 mg/kg dizocilpine maleate (MK-801) treatment group(n=6）, extracted neutrophils separately, and observed the morphology and counted under a microscope. Each group of cells was divided into two parts for chemotactic experiment, called chemokine agent treatment group and no chemokine agent treatment group respectively, indicating control 1,0.3 mg/kg MK801 treatment 1,0.6 mg/kg MK-801 treatment 1 and control 2,0.3 mg/kg MK-801 treatment 2,0.6 mg/kg MK-801 treatment 2.The dynamic migration of cells was recorded using the NIS-Elements software, and TAXIScan Analyzer 2 software was used to select 30 cells (n=30) in each group of cells and analyze cells migration trajectory, speed and distance, and use pair test and One-Way analysis of variance for statistical analysis. Results: The number of neutrophils in control group, 0.3 mg/kg MK-801 treatment group and 0.6 mg/kg MK-801 treatment group were（1.00±0.03）×104/mL,（0.05±0.02）×104/mL,（0.32±0.01）×104/mL respectively, the differences of results were statistically significant（P<0.05）.Under the effect of chemotactic agent,the directional migration capability of neutrophils in control group 1, 0.3 mg/kg MK-801 treatment group 1 and 0.6 mg/kg MK-801 treatment group 1 were（0.85±0.11） radian,（1.00±0.11） radian,（0.96±0.10） radian respectively （P<0.05）; the migration velocities of neutrophils were （0.09±0.02） μm/s,（0.12±0.01） μm/s,（0.14±0.01） μm/s respectively （P<0.05）;the migration distances of neutrophils were （94.26±0.02） μm,（134.61±0.01） μm,（156.19±0.01） μm respectively（P<0.05）. Conclusion: Compared with neutrophils in peritoneal cavity of control group, the neutrophils in peritoneal cavity of schizophrenic rats have stronger chemotactic movement ability.