目的： 探讨粉防己碱（tetrandrine，Tet）对大鼠心肌成纤维细胞增殖、活化的影响。方法： 采用细胞计数试剂盒（cell counting kit-8C，CCK-8）的方法检测不同浓度Tet对心肌成纤维细胞增殖的抑制作用；采用蛋白质印迹（Western blot）法检测Tet对由转化生长因子β(transforming growth factor-β，TGF-β）诱导的心肌成纤维细胞 β-链蛋白（β-catenin）、波形蛋白（vimentin，Vm）、纤连蛋白（fibronectin，Fn）及平滑肌α肌动蛋白（smooth muscle α-actin，SMA）的表达上调的影响作用；采用实时荧光定量PCR的方法检测Tet作用下，TGF-β诱导的心肌成纤维细胞Ⅰ型胶原（collagen-1，Col-1）和Ⅲ型胶原（collagen-3，Col3）的表达上调的变化。结果：CCK-8检测结果显示，分别用0.5、1、2、4、8 μmol/L的Tet处理心肌成纤维细胞，其增殖活力分别下降至对照组的94.4%、84.9%、74.9%、63.8%、50.3%，差异有统计学意义（所有P值<0.05；1、2、4、8 μmol/L处理组P值<0.001）。Western blot检测结果显示：5 μg/L 的TGF-β显著上调β-catenin、Vm、Fn及SMA的表达（P值分别为0.001、0.008、0.010、0.001），而用0.5 μmol/L的Tet预处理心肌成纤维细胞后，可以阻断由TGF-β诱导的β-catenin、Fn、SMA的表达上调（P值分别为0.009、0.005、0.019），但不改变Vm的表达变化（P>0.05）。荧光定量PCR检测结果表明：用0.5 μmol/L的Tet预处理心肌成纤维细胞，可以减弱由TGFβ诱导的Ⅰ型及Ⅲ型胶原的表达上调，差异有统计学意义（P<0.001）。结论：Tet可以显著抑制心肌成纤维细胞的增殖，并阻断由TGF-β诱导的心肌成纤维细胞活化,据此推断，Tet可能具有抗心肌纤维化的作用，进而阻断心肌重塑。

Objective： To investigate the effects of tetrandrine (Tet) on proliferation and activation of rat cardiac fibroblasts. Methods： Firstly, the cell counting kit-8（cck-8） assay was applied to detect the effects of Tet with different concentrations on proliferation of cardiac fibroblasts. Secondly, transforming growth factor (TGF-β)with a concentration of 5 μg/L was used to induce the cardiac fibroblast activation, and Western blot was performed to measure the expression variation of βcatenin, vimentin (Vm), fibronectin (Fn) and smooth muscle α-actin (SMA). At last, the real-time PCR was conducted to mea-sure the expression change of collagen-1(Col-1) and collagen-3(Col-3). Results: The cck8 assay showed that the Tet with different concentrations respectively, which were 0.5 μmol/L, 1 μmol/L, 2 μmol/L, 4 μmol/L, and 8 μmol/L, significantly inhibited the proliferation of cardiac fibroblasts. The viability was decreased to 94.4%,84.9%,74.9%,63.8%and 50.3% respectively of the control group when the Tet concentration changed, and the difference was statistically significant, P=0.043，P<0.001，P<0.001，P<0.001，P<0.001 respectively. Western blot revealed that the expressions of β-catenin, Fn, SMA and Vm, were up-regulated by TGF-β(5 μg/L), the result showed that the difference was statistically significant, and the P values were 0.001，0.008，0.010，0.001 respectively. Then, the up-regulation of β-catenin, Fn and SMA was attenuated by pre-treatment of Tet, and the result also displayed that the difference was statistically significant, and the P values were 0.009，0.005，0.019,respectively. While there was no significant change in the expression of Vm, according to Western blotting, and P>0.05,at the same time, realtime PCR indicated that the up-regulations of Col-1 and Col-3 which were induced by TGF-β were blocked by pretreatment of Tet, the result showed that the difference was statistically significant, P<0.001. Conclusion: According to the experimental results, we can draw the conclusion that: the Tet can significantly inhibit the proliferation of cardiac fibroblasts, meanwhile, it can block the activation of cardiac fibroblasts, which is induced by TGF-β. It is supposed that the Tet may probably have anti myocardial fibrosis, which indicates that it may probably be a medicine which is used to block the cardiac remodeling.