收稿日期: 2020-09-29
网络出版日期: 2021-02-07
Association between root abnormalities and related pathogenic genes in patients with generalized aggressive periodontitis
Received date: 2020-09-29
Online published: 2021-02-07
目的: 探索广泛型侵袭性牙周炎(generalized aggressive periodontitis,GAgP)患者牙根形态异常与骨代谢或牙根发育相关基因多态性的关联。方法: 纳入179例GAgP患者,平均(27.23±5.19)岁,男 ∶女=67 ∶112,平均存留牙数(26.80±1.84)颗。采用基于基质辅助激光解吸电离飞行时间质谱技术进行 9个与骨代谢和牙根发育相关基因的13个单核苷酸多态性位点(single nucleotide polymorphisms,SNPs)的基因型检测。采用全口根尖片评判牙根形态异常,包括锥根、细长根、冠根比例失调、弯曲根、融合根、后牙根形态异常,分析13个SNPs位点不同基因型根形态异常牙的数量及发生率。结果: GAgP患者根形态异常牙构成比为14.49%(695/4 798颗),平均(3.88±3.84)颗。维生素D受体(vitamin D receptor,VDR)基因rs2228570位点的CC、CT、TT基因型患者根形态异常牙数量分别为(4.66±4.10)、(3.71±3.93)和(2.68±2.68)颗,CC基因型和TT基因型之间差异有统计学意义(t=2.62,P=0.01)。降钙素受体(calcitotin receptor,CTR)基因rs2283002位点CC、CT、TT基因型患者根形态异常数分别为(5.02±3.70)、(3.43±3.95)、(3.05±3.12)颗,CC基因型的根形态异常发病率高于CT和TT基因型(87.86% vs. 65.26%和63.64%,P=0.006,adjusted OR=3.71,95%CI:1.45~9.50)。结论: VDR rs2228570及CTR rs2283002位点可能与广泛型侵袭性牙周炎患者牙根形态异常的发生有关,值得进一步研究。
刘建 , 王宪娥 , 吕达 , 乔敏 , 张立 , 孟焕新 , 徐莉 , 毛铭馨 . 广泛型侵袭性牙周炎患者牙根形态异常与相关致病基因的关联[J]. 北京大学学报（医学版）, 2021 , 53(1) : 16 -23 . DOI: 10.19723/j.issn.1671-167X.2021.01.004
Objective: To explore the association between the abnormal root morphology and bone metabolism or root development related gene polymorphism in patients with generalized aggressive periodontitis.Methods: In the study, 179 patients with generalized aggressive periodontitis were enrolled, with an average age of (27.23±5.19) years, male / female = 67/112. The average number of teeth remaining in the mouth was (26.80±1.84). Thirteen single nucleotide polymorphisms (SNPs) of nine genes which related to bone metabolism and root development were detected by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Root abnormalities were identified using periapical radiographs. The abnormal root morphology included cone-rooted teeth,slender-root teeth,short-rooted teeth,curved-rooted teeth,syncretic-rooted molars,and molar root abnormalities. The number of teeth and incidence of abnormal root morphology in different genotypes of 13 SNPs were analyzed.Results: The constituent ratio of root with root abnormality in GAgP patients was 14.49%(695/4 798). The average number of teeth with abnormal root morphology in GAgP was (3.88±3.84). The average number of teeth with abnormal root morphology in CC, CT and TT genotypes in vitamin D receptor (VDR) rs2228570 was (4.66±4.10), (3.71±3.93) and (2.68±2.68). There was significant difference between TT genotype and CC genotype (t = 2.62, P =0.01). The average number of root morphological abnormalities in CC, CT and TT genotypes of Calcitotin Receptor (CTR) gene rs2283002 was (5.02±3.70),(3.43±3.95), and (3.05±3.12). The incidence of root morphological abnormalities in CC genotype was higher than that in the patients with CT and TT, and the difference was statistically significant(87.86% vs. 65.26% & 63.64%, P=0.006,adjusted OR =3.71,95%CI: 1.45-9.50). There was no significant difference in the incidence of abnormal root morphology between CT and TT genotypes.Conclusion: VDR rs2228570 and CTR rs2283002 may be associated with the occurrence of abnormal root morphology in patients with generalized aggressive periodontitis, which is worthy of further research.
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