收稿日期: 2021-01-26
网络出版日期: 2024-07-23
基金资助
国家自然科学基金(91643111);国家自然科学基金(91743114)
Effects of PM2.5 and O3 sub-chronic combined exposure on ATP amount and ATPase activities in rat nasal mucosa
Received date: 2021-01-26
Online published: 2024-07-23
Supported by
the National Natural Science Foundation of China(91643111);the National Natural Science Foundation of China(91743114)
目的: 探讨细颗粒物(fine particle matter，PM2.5)和臭氧(ozone，O3)亚慢性共同暴露对大鼠鼻黏膜组织腺嘌呤核苷三磷酸(adenosine triphosphate, ATP)总量及ATP酶活性的影响。方法: 采用随机数字表法将20只雄性Sprague Dawley (SD) 大鼠均分为对照组和暴露组，每组各10只，分别饲养于常规清洁级环境和本团队既往所搭建的大气污染物暴露系统中，连续暴露208 d。暴露期间，监测暴露系统内PM2.5和O3浓度，采用自测和站点数据相结合的方法对暴露系统内PM2.5和O3进行综合评估。在暴露第208天处死大鼠取心、肝、脾、肾、睾丸等主要器官和鼻黏膜组织，称量各脏器重量并计算脏器系数。利用所收集鼻黏膜组织，使用生物发光法测定ATP总量，使用分光光度法检测Na+-K+-ATP酶和Ca2+-ATP酶活性。使用两独立样本t检验比较各指标组间差异。结果: 自第3周开始至暴露结束，暴露组大鼠体质量高于对照组(P < 0.05)，两组间脏器系数差异无统计学意义。暴露组日均PM2.5浓度为(30.68 ± 19.23) μg/m3，O3最大8 h浓度(O3-8 h)为(82.45 ± 35.81) μg/m3。暴露组大鼠鼻黏膜组织ATP化学发光值(792.4±274.1) IU/L低于对照组(1 126.8±218.1) IU/L，鼻黏膜组织Na+-K+-ATP酶活性(1.53±0.85) U/mg低于对照组(4.31±1.60) U/mg(P < 0.05)。对照组和暴露组鼻黏膜组织的蛋白含量分别为(302.14±52.51) mg/L和(234.58±53.49) mg/L，Ca2+-ATP酶活性分别为(0.81±0.27) U/mg和(0.99± 0.73) U/mg，组间差异均无统计学意义。结论: 亚慢性PM2.5和O3共同暴露可能影响大鼠鼻黏膜组织供氧能力。
阎腾龙 , 胥嘉钰 , 陈田 , 杨鑫 , 王伟伟 , 周淑佩 , 牛丕业 , 贾光 , 夏交 . 亚慢性PM2.5和O3共同暴露对大鼠鼻黏膜ATP总量及ATP酶活性的影响[J]. 北京大学学报（医学版）, 2024 , 56(4) : 687 -692 . DOI: 10.19723/j.issn.1671-167X.2024.04.023
Objective: To evaluate the effects of fine particle matter (PM2.5) and ozone (O3) combined exposure on adenosine triphosphate (ATP) amount and ATPase activities in nasal mucosa of Sprague Dawley (SD) rats. Methods: Twenty male SD rats were divided into control group (n=10) and exposure group (n=10) by random number table method. The rats were fed in the conventional clean environment and the air pollutant exposure system established by our team, respectively, and exposed for 208 d. During the exposure period, the concentrations of PM2.5 and O3 in the exposure system were monitored, and a comprehensive assessment of PM2.5 and O3 in the exposure system was conducted by combining self-measurement and site data. On the 208 d of exposure, the core, liver, spleen, kidney, testis and other major organs and nasal mucosal tissues of the rats were harvested. Each organ was weighed and the organ coefficient calculated. The total amount of ATP was measured by bioluminescence, and the activities of Na+-K+ -ATPase and Ca2+ -ATPase were detected by spectrophotometry. The t test of two independent samples was used to compare the differences among the indicator groups. Results: From the 3rd week to the end of exposure duration, the body weight of the rats in the exposure group was higher than that in the control group (P < 0.05), and there was no significant difference in organ coefficients between the two groups. The average daily PM2.5 concentration in the exposure group was (30.68±19.23) μg/m3, and the maximum 8 h ozone concentration (O3-8 h) was (82.45±35.81) μg/m3. The chemiluminescence value (792.4±274.1) IU/L of ATP in nasal mucosa of the rats in the exposure group was lower than that in the control group (1 126.8±218.1) IU/L. The Na+-K+-ATPase activity (1.53±0.85) U/mg in nasal mucosa of the rats in the exposure group was lower than that in the control group (4.31±1.60) U/mg (P < 0.05). The protein content of nasal mucosa in the control group and the exposure group were (302.14±52.51) mg/L and (234.58±53.49) mg/L, respectively, and the activity of Ca2+-ATPase was (0.81±0.27) U/mg and (0.99±0.73) U/mg, respectively. There was no significant difference between the groups. Conclusion: The ability of power capacity decreased in the rat nasal mucossa under the sub-chronic low-concentration exposure of PM2.5 and O3.
Key words： Fine particle matter; Ozone; Adenosine triphosphate
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