目的: 明确浓缩生长因子(concentrated growth factors，CGF)在自固化磷酸钙人工骨[采用磷酸钙骨水泥(calcium phosphate cement，CPC)为载体]治疗牙周骨下袋缺损中的作用。方法: 对36例骨下袋缺损进行随机分组，试验组采用CGF+CPC进行治疗(n=18)，对照组单独使用CPC进行治疗(n=18)。在基线和术后1年时评估两组的探诊深度、临床附着水平，锥形束CT(cone beam CT，CBCT)检测硬组织充填情况，比较CGF和血清中主要生长因子的水平(血小板衍生生长因子-BB、转化生长因子β1、胰岛素样生长因子1、血管内皮生长因子)。结果: 基线时两组的探诊深度、临床附着水平和CBCT硬组织充填测量值方面差异均无统计学意义(P>0.05)。术后1年时，两组在探诊深度、临床附着丧失和CBCT硬组织充填测量值方面均有显著改善(P < 0.05)。CGF+CPC在探诊深度降低[(4.5±1.3) mm vs. (3.2±1.1) mm]和临床附着获得[(3.8±0.9) mm vs. (2.0±0.5) mm]方面比单独使用CPC更有效(P < 0.05)。与单纯CPC组相比，CGF+CPC组CBCT显示硬组织充填程度明显增加[骨袋深减少(3.9±1.2) mm vs. (2.1±0.7) mm, P < 0.01]。术后1年时，CGF+CPC组CBCT显示骨下袋体积减少(0.031 8± 0.004 1) mL，显著优于单纯CPC组[(0.019 7±0.001 2) mL, P < 0.05]。此外，CGF中主要生长因子的含量高于血清(P < 0.001)。结论: 术后1年的观察结果显示，CGF可显著促进CPC治疗牙周骨下袋缺损的临床效果和影像学效果。

Objective: To clarify the role of concentrated growth factors (CGF) in the treatment of periodontal cement defects using calcium phosphate cement (CPC) with self-curing properties. Methods: Thirty-six intrabony defects were randomly divided into two groups. The experimental group received CGF+CPC treatment (n=18), while the control group received CPC treatment alone (n=18). The probing depth, clinical attachment loss, and hard tissue filling as measured by cone beam CT (CBCT) were evaluated at baseline and 1 year postoperatively in both groups, and the levels of major growth factors in CGF and serum were compared [platelet-derived growth factor-BB (PDGF-BB), transforming growth factor-β1 (TGF-β1), insulin-like growth factor-1 (IGF-1), and vascular endothelial growth factor (VEGF)]. Results: At baseline, there were no statistically significant differences in probing depth, clinical attachment loss and CBCT measurements between the two groups (P>0.05). At 1 year postoperatively, significant improvements were observed in parameters mentioned above in both groups (P < 0.05). The CGF+CPC group seemed more effective compared with the CPC group in reduction of probing depth [(4.5±1.3) mm vs. (3.2±1.1) mm] and clinical attachment gain [(3.8±0.9) mm vs. (2.0±0.5) mm, P < 0.05]. Compared with the group treated with CPC alone, the hard tissue filling degree shown by CBCT in the CGF+CPC group was significantly increased [the reduction of the depth of the intrabony defects was (3.9±1.2) mm vs. (2.1±0.7) mm, respectively, P < 0.01]. At 1 year post-operatively, the volume of the intrabony defects shown by CBCT in the CGF+CPC group was reduced by (0.031 8±0.004 1) mL, which was significantly more than that in the CPC group [(0.019 7±0.001 2) mL, P < 0.05]. In addition, the concentration of the main growth factors (PDGF-BB, TGF-β1, IGF-1, and VEGF) in CGF were higher than those in serum (P < 0.001). Conclusion: After 1 year of follow-up, the results of the present study indicated that CGF could significantly improve the clinical and radiological effects of CPC on the treatment of periodontal intrabony defects.