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Journal of Peking University (Health Sciences) ›› 2020, Vol. 52 ›› Issue (5): 952-958. doi: 10.19723/j.issn.1671-167X.2020.05.027

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Preparation and osteogenic effect study of small intestinal submucosa sponge

Mei WANG1*,Bo-wen LI1*,Si-wen WANG2,Yu-hua LIU1,∆()   

  1. 1. Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China
    2. Department of Prosthodontics, Tongji University School and Hospital of Stomatology, Shanghai 200072, China
  • Received:2018-10-09 Online:2020-10-18 Published:2020-10-15
  • Contact: Yu-hua LIU E-mail:liuyuhua@bjmu.edu.cn

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Abstract:

Objective: To prepare and evaluate the basic properties in vitro of a novel small intestinal submucosa (SIS) sponge, and to describe the bone formation ability of the SIS sponge in vivo. Methods: The SIS sponge was prepared by freeze-drying method. To evaluate the physicochemical properties of the sponge, electron microscope observation, porosity test, water absorption ability and mechanical property were conducted in vitro. The cytotoxicity of the SIS sponge was performed by cell counting kit-8 method. In vivo experiments, eighteen extraction sockets of premolar of three Beagle dogs were randomly divided into three groups: SIS sponge group (SIS sponge), positive control group (Bio-Oss granules and Bio-Gide membrane) and control group(no treatment). The animals were sacrificed 4 weeks and 12 weeks after operation, and micro computed tomography (Micro-CT) was applied to measure the bone volume fraction (BV/TV) and bone mineralized density (BMD). The data were analyzed with one-way ANOVA. Results: The average pore diameter of the SIS sponge was (194.90±30.39) μm, the porosity was 92.31%±0.24%, the water absorption rate was 771.50%±40.90%, and the compressive elastic modulus was (2.20±0.19) kPa. There was no significant difference in cell proliferation ability between SIS sponge and control group (P>0.05). Micro-CT quantitative results showed that BV/TV of SIS sponge group (52.81%±3.21%) and positive control group (58.30%±9.36%) were significantly higher than that of control group (38.65%±4.80%) 4 weeks after operation (P <0.05). The BMD of SIS sponge group [(887.09±61.02) mg/cm3], positive control group [(952.05±132.78) mg/cm3] and control group [(879.29±74.27) mg/cm3] showed no statistical difference 4 weeks after operation (P>0.05). The BV/TV of positive control group (60.57%± 6.56%) was significantly higher than that of SIS sponge group (47.89%±3.59%) and control group (42.99%±2.54%) 12 weeks after operation (P < 0.05). BMD of SIS sponge group [(1047±89.95) mg/cm3] and positive control group [(1101.37±98.85) mg/cm3] were significantly higher than that of control group [(890.36±79.79) mg/cm3] 12 weeks after operation (P <0.05). Conclusion: The SIS sponge has satisfying physicochemical properties and biocompatibility. The SIS sponge significantly increased bone volume fraction in the early stage of bone formation (4 weeks) and bone mineralized density in the late stage of bone formation (12 weeks).

Key words: Small intestinal submucosa sponge, Bone tissue engineering, Osteogenesis

CLC Number: 

  • R783.3

Figure 1

Operation process A, preoperation;B, pulp opening;C, extraction;D, suture. "

Figure 2

Physical appearance of SIS sponge"

Figure 3

Scanning electron microscope results of SIS sponge A,×300;B,×500."

Figure 4

Effect of SIS sponge on proliferation of hBMSCs SIS, small intestinal submucoma. "

Figure 5

Micro-CT images at 4 weeks after operation A, B, C, SIS sponge group;D, E, F, positive control group;G, H, I, control group. "

Figure 6

Micro-CT images at 12 weeks after operation A, B, C, SIS sponge group;D, E, F, positive control group;G, H, I, control group. "

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