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Journal of Peking University(Health Sciences) ›› 2018, Vol. 50 ›› Issue (5): 868-875. doi: 10.19723/j.issn.1671-167X.2018.05.018

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Effects of low level laser irradiation on the osteogenic capacity of sodium alginate/gelatin/human adipose-derived stem cells 3D bio-printing construct

SUI Hua-xin1, LV Pei-jun2△, WANG Yong2, FENG Yu-chi1   

  1. (1.Department of Stomatology, Peking University First Hospital, Beijing 100034, China; 2. Center of Digital Dentistry, Department of Prosthodontics, Peking University School and Hospital of Stomatology & Research Center of Engineering and Technology for Digital Dentistry, Ministry of Health & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China)
  • Online:2018-10-18 Published:2018-10-18
  • Contact: LV Pei-jun E-mail: kqlpj@bjmu.edu.cn
  • Supported by:
    Supported by Project of Chinese Ministry of Education，China (113002A)

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Abstract: Objective： To explore the effects of low level laser irradiation (LLLI) on the osteogenic capacity of three-dimensional （3D） structure by 3D bio-printing construct used human adipose-derived stem cells (hASCs) as seed cells. Methods: Using hASCs as seed cells, we prepared sodium alginate/gelatin/hASCs 3D bio-printing construct, and divided them into four groups: PM (proliferative medium), PM+LLLI, OM (osteogenic medium) and OM+LLLI, and the total doses of LLLI was 4 J/cm2. Immunofluorescence microscopy was used to observe the viability of the cells, and analyze the expression of the osteogenesis-related protein Runt-related transcription factor 2 (Runx2) and osteocalcin (OCN). Results: The 3D constructs obtained by printing were examined by microscope. The sizes of these 3D constructs were 10 mm×10 mm×1.5 mm. The wall thickness of the printed gelatin mold was approximately 1 mm, and the pores were round and had a diameter of about 700 μm. The cell viability of sodium alginate/gelatin/hASCs 3D bio-printing construct was high, and the difference among the four groups was not significant. On day 7, the expression of OCN from high to low was group OM+LLLI, PM+LLLI, OM and PM. There were significant differences among these groups (P<0.01), but there was no significant difference between group PM+LLLI and OM. On day 14, the expression of OCN in each group was hi-gher than that on day 7, and there was no significant difference between group OM+LLLI and OM. The expression of Runx2 in group OM+LLLI was more than 90%, significantly higher than that in group OM (P<0.01). But the expression of Runx2 in group PM+LLLI and OM+LLLI were significantly lower than that in the non-irradiated groups. The expression of osteogenesis-related protein Runx2 and OCN were higher in OM groups than in PM groups. Furthermore, the irradiated groups were significantly higher than the non-irradiated groups. Conclusion: LLLI does not affect the cell viability of sodium alginate/gelatin/hASCs 3D bio-printing construct, and may promote the osteogenic differentiation of hASCs.

Key words: Low-level laser irradiation, Bioprinting, Cell differentiation, Osteogenesis, Stem cells

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