Email Alert | RSS

Journal of Peking University(Health Sciences) ›› 2018, Vol. 50 ›› Issue (5): 868-875. doi: 10.19723/j.issn.1671-167X.2018.05.018

• Article • Previous Articles     Next Articles

Effects of low level laser irradiation on the osteogenic capacity of sodium alginate/gelatin/human adipose-derived stem cells 3D bio-printing construct

SUI Hua-xin1, LV Pei-jun2△, WANG Yong2, FENG Yu-chi1   

  1. (1.Department of Stomatology, Peking University First Hospital, Beijing 100034, China; 2. Center of Digital Dentistry, Department of Prosthodontics, Peking University School and Hospital of Stomatology & Research Center of Engineering and Technology for Digital Dentistry, Ministry of Health & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China)
  • Online:2018-10-18 Published:2018-10-18
  • Contact: LV Pei-jun E-mail: kqlpj@bjmu.edu.cn
  • Supported by:
    Supported by Project of Chinese Ministry of Education，China (113002A)

RICH HTML

0   

PDF (PC)

352

Abstract: Objective： To explore the effects of low level laser irradiation (LLLI) on the osteogenic capacity of three-dimensional （3D） structure by 3D bio-printing construct used human adipose-derived stem cells (hASCs) as seed cells. Methods: Using hASCs as seed cells, we prepared sodium alginate/gelatin/hASCs 3D bio-printing construct, and divided them into four groups: PM (proliferative medium), PM+LLLI, OM (osteogenic medium) and OM+LLLI, and the total doses of LLLI was 4 J/cm2. Immunofluorescence microscopy was used to observe the viability of the cells, and analyze the expression of the osteogenesis-related protein Runt-related transcription factor 2 (Runx2) and osteocalcin (OCN). Results: The 3D constructs obtained by printing were examined by microscope. The sizes of these 3D constructs were 10 mm×10 mm×1.5 mm. The wall thickness of the printed gelatin mold was approximately 1 mm, and the pores were round and had a diameter of about 700 μm. The cell viability of sodium alginate/gelatin/hASCs 3D bio-printing construct was high, and the difference among the four groups was not significant. On day 7, the expression of OCN from high to low was group OM+LLLI, PM+LLLI, OM and PM. There were significant differences among these groups (P<0.01), but there was no significant difference between group PM+LLLI and OM. On day 14, the expression of OCN in each group was hi-gher than that on day 7, and there was no significant difference between group OM+LLLI and OM. The expression of Runx2 in group OM+LLLI was more than 90%, significantly higher than that in group OM (P<0.01). But the expression of Runx2 in group PM+LLLI and OM+LLLI were significantly lower than that in the non-irradiated groups. The expression of osteogenesis-related protein Runx2 and OCN were higher in OM groups than in PM groups. Furthermore, the irradiated groups were significantly higher than the non-irradiated groups. Conclusion: LLLI does not affect the cell viability of sodium alginate/gelatin/hASCs 3D bio-printing construct, and may promote the osteogenic differentiation of hASCs.

Key words: Low-level laser irradiation, Bioprinting, Cell differentiation, Osteogenesis, Stem cells

CLC Number: 

  •  
[1] Wen-yu DU,Jing-wen YANG,Ting JIANG. Early constant observation of the effect of deferoxamine mesylate on improvement of vascularized bone regeneration in SD rat skull critical size defect model [J]. Journal of Peking University (Health Sciences), 2021, 53(6): 1171-1177.
[2] WANG Jing-qi,WANG Xiao. In vivo study of strontium-doped calcium phosphate cement for biological properties [J]. Journal of Peking University (Health Sciences), 2021, 53(2): 378-383.
[3] ZHANG Sheng-nan,AN Na,OUYANG Xiang-ying,LIU Ying-jun,WANG Xue-kui. Role of growth arrest-specific protein 6 in migration and osteogenic differentiation of human periodontal ligament cells [J]. Journal of Peking University (Health Sciences), 2021, 53(1): 9-15.
[4] Mei WANG, Bo-wen LI, Si-wen WANG, Yu-hua LIU. Preparation and osteogenic effect study of small intestinal submucosa sponge [J]. Journal of Peking University (Health Sciences), 2020, 52(5): 952-958.
[5] Xiang-song BAI,Long-wei LV,Yong-sheng ZHOU. Tribbles pseudokinase 3 inhibits the adipogenic differentiation of human adipose-derived mesenchymal stem cells [J]. Journal of Peking University(Health Sciences), 2020, 52(1): 1-9.
[6] Xiao-min GAO,Xiao-ying ZOU,Lin YUE. Mediated pathways of exosomes uptake by stem cells of apical papilla [J]. Journal of Peking University(Health Sciences), 2020, 52(1): 43-50.
[7] Xin-yun YAO,Xiao-min GAO,Xiao-ying ZOU,Lin YUE. Role of endocytosis in cell surface CXC chemokine receptor 4 expression of stem cells from apical papilla [J]. Journal of Peking University(Health Sciences), 2019, 51(5): 893-899.
[8] Jing XIE,Yu-ming ZHAO,Nan-quan RAO,Xiao-tong WANG,Teng-jiao-zi FANG,Xiao-xia LI,Yue ZHAI,Jing-zhi LI,Li-hong GE,Yuan-yuan WANG. Comparative study of differentiation potential of mesenchymal stem cells derived from orofacial system into vascular endothelial cells [J]. Journal of Peking University(Health Sciences), 2019, 51(5): 900-906.
[9] Ying CHEN,Zhong-ning LIU,Bo LI,Ting JIANG. Preparation of aspirin sustained-release microsphere and its in vitro releasing [J]. Journal of Peking University(Health Sciences), 2019, 51(5): 907-912.
[10] Xia LIU,Ying ni LI,Xiao li SUN,Qing lin PENG,Xin LU,Guo chun WANG. Effects of integrin metalloproteinases on osteogenic differentiation [J]. Journal of Peking University(Health Sciences), 2018, 50(6): 962-967.
[11] WANG Zi-cheng, CHENG Li, LV Tong-de, SU Li, LIN Jian, ZHOU Li-qun. Inflammatory priming adipose derived stem cells significantly inhibit the proliferation of peripheral blood mononuclear cells [J]. Journal of Peking University(Health Sciences), 2018, 50(4): 590-594.
[12] WANG Xiao-tong, RAO Nan-quan, FANG Teng-jiao-zi, ZHAO Yu-ming, GE Li-hong. Comparison of the properties of CD146 positive and CD146 negative subpopulations of stem cells from human exfoliated deciduous teeth [J]. Journal of Peking University(Health Sciences), 2018, 50(2): 284-292.
[13] LIU Jing-yin, CHEN Fei, GE Yan-jun, WEI Ling, PAN Shao-xia, FENG Hai-lan. Influence of implants prepared by selective laser melting on early bone healing [J]. Journal of Peking University(Health Sciences), 2018, 50(1): 117-122.
[14] YANG Rui-li, YU Ting-ting, ZHOU Yan-heng. Acetylsalicylic acid treatment enhanced immunomodulatory function of mesenchymal stem cells derived from gingiva [J]. Journal of Peking University(Health Sciences), 2017, 49(5): 872-877.
[15] JIA Wei-qian, ZHAO Yu-ming, GE Li-hong. Recombinant human transforming growth factor β1 promotes dental pulp stem cells proliferation and mineralization [J]. Journal of Peking University(Health Sciences), 2017, 49(4): 680-681.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Author. English Title Test[J]. Journal of Peking University(Health Sciences), 2010, 42(1): 1 -10 .
[2] . [J]. Journal of Peking University(Health Sciences), 2009, 41(2): 188 -191 .
[3] . [J]. Journal of Peking University(Health Sciences), 2009, 41(3): 376 -379 .
[4] . [J]. Journal of Peking University(Health Sciences), 2009, 41(4): 459 -462 .
[5] . [J]. Journal of Peking University(Health Sciences), 2010, 42(1): 82 -84 .
[6] . [J]. Journal of Peking University(Health Sciences), 2007, 39(3): 319 -322 .
[7] . [J]. Journal of Peking University(Health Sciences), 2007, 39(3): 333 -336 .
[8] . [J]. Journal of Peking University(Health Sciences), 2007, 39(3): 337 -340 .
[9] . [J]. Journal of Peking University(Health Sciences), 2007, 39(3): 225 -328 .
[10] . [J]. Journal of Peking University(Health Sciences), 2007, 39(4): 346 -350 .
Copyright © Journal of Peking University (Health Sciences), All Rights Reserved.
Powered by Beijing Magtech Co. Ltd