Email Alert | RSS

Journal of Peking University (Health Sciences) ›› 2021, Vol. 53 ›› Issue (1): 46-53. doi: 10.19723/j.issn.1671-167X.2021.01.008

Previous Articles     Next Articles

Effects of three self-etch adhesives and mild salivary contamination on the bonding durability of deciduous teeth

LUO Chi-yi,PENG Chu-fang,YANG Yuan,QIN Man,WANG Yuan-yuan()   

  1. Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Disease & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China
  • Received:2020-09-21 Online:2021-02-18 Published:2021-02-07
  • Contact: Yuan-yuan WANG E-mail:cwyyd@126.com
  • Supported by:
    Chinese Stomatological Association Youth Clinical Research Foundation(CSA-B2018-10)

RICH HTML

   

PDF (PC)

Abstract:

Objective: To compare the bonding durability of three different self-etch adhesives to primary enamel and dentin, and to investigate the effect of mild salivary contamination followed by air drying on the bonding durability.Methods: Two hundred and forty enamel specimens were divided randomly into 16 groups (n=15/group)according to the adhesive system [self-etch adhesives: Clearfil SE Bond(SE), AdperTM Easy One (EO), Scotchbond Universal (SBU); total-etch adhesive: AdperTM Single Bond Plus(SL)], contamination status (non-contaminated vs. salivary-contaminated ) and storage condition (stored in distilled water for 24 h vs. aging mode 5 000 thermal cycles in 5 ℃ and 55 ℃). Two hundred and forty dentin specimens were assigned in the same way. Shear bond strength for 12 specimens in each group were measured. The adhesive interface for the residual specimens in each group was observed by scanning electron microscopy(SEM). Data were analyzed by three-way analysis of variance and Tukey test(P<0.05).Results: For primary enamel, total-etch adhesive showed higher initial shear bond strength values (28.92±1.83) MPa and shear bond strength values (27.27±3.03) MPa after thermal cycles compared with the other groups, and the difference between the groups was statistically significant (P<0.01). Shear bond strength values of EO decreased significantly in salivary-treated groups, regardless of storage conditions, and the difference was statistically significant (P<0.01). For primary dentin, shear bond strength values of EO decreased significantly in salivary-treated groups after 24 h (P<0.01). After 5 000 thermal cycles, total-etch adhesive showed significantly lower shear bond strength values (14.31±1.97) MPa compared with the other groups, and the difference between the groups was statistically significant (P<0.01), and shear bond strength values of EO were significantly lower than those in SE and SBU groups (P<0.01), regardless of contamination status.Conclusion: Total-etch adhesive SL has better bonding durability to primary enamel. SE and SBU have better bonding durability to primary dentin and have a certain resistance to salivary contamination, while the bonding performance of EO is compromised greatly by mild salivary contamination followed by air drying.

Key words: Primary teeth, Enamel, Dentin, Self-etch, Salivary contamination

CLC Number: 

  • R788.4

Figure 1

The establishment of bonding sample A, the applied adhesive system for setting up the bonding sample; B, a drop of saliva (6 μL) placed on the defined bonding area to establish the contaminated sample."

Table 1

Three-way analysis of variance for the SBS tests to primary enamel"

Factors Sum of squares df Mean square F-ratio P value
Adhesive 3 358.52 3 1 119.51 72.88 <0.001
Contamination 792.74 1 792.74 51.61 <0.001
Storage 0.06 1 0.06 0.00 0.952
Adhesive×contamination 180.41 3 60.14 3.92 0.010
Adhesive×storage 65.28 3 21.76 1.42 0.240
Contamination×storage 3.59 1 3.59 0.23 0.630
Adhesive×contamination×storage 6.11 3 2.04 0.13 0.941

Figure 2

Distribution of failure modes (primary enamel groups) I, initial; T, thermal cycling; N, non-contaminated; C, contaminated; SL, AdperTM Single Bond Plus; SE, Clearfil SE Bond; EO, AdperTM Easy One; SBU, Scotchbond Universal."

Figure 3

Representative scanning electron microscope images resin-enamel interfaces with salivary contamination A, B, resin-enamel interfaces with salivary contamination were observed by SEM after 5 000 thermal cycles in 5 ℃ and 55 ℃, representative SEM images of SL group at magnifications of ×500 and ×1 500; C, D, representative SEM images of SE group at magnifications of ×500 and ×1 500; E, F, representative SEM images of EO group at magnifications of ×500 and ×1 500; G, H, representative SEM images of SBU group at magnifications of ×500 and ×1 500. SL, AdperTM Single Bond Plus; SE, Clearfil SE Bond; EO, AdperTM Easy One; SBU, Scotchbond Universal; SEM, scanning electron microscope."

Table 2

Three-way analysis of variance for the SBS tests to primary dentin"

Factors Sum of squares df Mean square F-ratio P value
Adhesive 1 392.80 3 464.27 32.56 <0.001
Contamination 295.43 1 295.43 20.72 <0.001
Storage 22.86 1 22.86 1.60 0.207
Adhesive×contamination 74.57 3 24.86 1.74 0.160
Adhesive×storage 341.00 3 113.67 7.97 <0.001
Contamination×storage 9.11 1 9.11 0.64 0.425
Adhesive×contamination×storage 13.17 3 4.39 0.31 0.820

Figure 4

Distribution of failure modes (primary dentin groups) I, initial; T, thermal cycling; N, non-contaminated; C, contaminated; SL, AdperTM Single Bond Plus; SE, Clearfil SE Bond; EO, AdperTM Easy One; SBU, Scotchbond Universal."

Figure 5

Representative scanning electron microscopy images resin-dentin interfaces with salivary contamination A, B, resin-dentin interfaces with salivary contamination were observed by SEM after 5 000 thermal cycles in 5 ℃ and 55 ℃, representative SEM images of SL group at magnifications of ×500 and ×1 500; C, D, representative SEM images of SE group at magnifications of ×500 and ×1 500; E, F, representative SEM images of EO group at magnifications of ×500 and ×1 500; G, H, representative SEM images of SBU group at magnifications of ×500 and ×1 500. SL, AdperTM Single Bond Plus; SE, Clearfil SE Bond; EO, AdperTM Easy One; SBU, Scotchbond Universal; SEM, scanning electron microscope."

[1] Perdigao J, Sezinando A, Monteiro PC. Laboratory bonding ability of a multi-purpose dentin adhesive[J]. Am J Dent, 2012,25(3):153-158.
[2] Munoz MA, Luque-Martinez IV, Malaquias P, et al. In vitro longe-vity of bonding properties of universal adhesives to dentin[J]. Oper Dent, 2015,40(3):282-292.
doi: 10.2341/14-055-L pmid: 25405904
[3] Lawson NC, Robles A, Fu CC, et al. Two-year clinical trial of a universal adhesive in total-etch and self-etch mode in non-carious cervical lesions[J]. J Dent, 2015,43(10):1229-1234.
doi: 10.1016/j.jdent.2015.07.009 pmid: 26231300
[4] Vermelho PM, Reis AF, Ambrosano GMB, et al. Adhesion of multimode adhesives to enamel and dentin after one year of water storage[J]. Clinical Oral Investigations, 2016,21(5):1707-1715.
doi: 10.1007/s00784-016-1966-1 pmid: 27714528
[5] Santschi K, Peutzfeldt A, Lussi A, et al. Effect of salivary contamination and decontamination on bond strength of two one-step selfetching adhesives to dentin of primary and permanent teeth[J]. J Adhes Dent, 2015,17(1):51-57.
doi: 10.3290/j.jad.a33514 pmid: 25625136
[6] Soares FZM, Rocha RdO, Raggio DP, et al. Microtensile bond strength of different adhesive systems to primary and permanent dentin[J]. Pediatr Dent, 2005,27(6):457-462.
pmid: 16532885
[7] Ozmen B, Koyuturk AE, Tokay U, et al. Evaluation of bond strength of self-etching adhesives having different pH on primary and permanent teeth dentin[J]. J Appl Biomater Funct Mater, 2015,13(3):e274-e279.
doi: 10.5301/jabfm.5000234 pmid: 26391869
[8] Osorio R, Yamauti M, Ruiz-Requena ME, et al. MMPs activity and bond strength in deciduous dentine-resin bonded interfaces[J]. J Dent, 2013,41(6):549-555.
doi: 10.1016/j.jdent.2013.02.008 pmid: 23454331
[9] Soares FZ, Lenzi TL, de Oliveira Rocha R. Degradation of resin-dentine bond of different adhesive systems to primary and permanent dentine[J]. Eur Arch Paediatr Dent, 2017,18(2):113-118.
doi: 10.1007/s40368-017-0282-z pmid: 28271448
[10] Yaguchi T. Layering mechanism of MDP-Ca salt produced in demineralization of enamel and dentin apatite[J]. Dent Mater, 2017,33(1):23-32.
doi: 10.1016/j.dental.2016.09.037 pmid: 27773341
[11] Kulkarni AS, Kokate S, hegde V, et al. The effect of saliva con-tamination on shear bond strength of two universal bonding agents: an in vitro study[J]. J Clin Diagn Res, 2018,12(4):6-10.
[12] Cobanoglu N, Unlu N, Ozer F, et al. Bond strength of self-etch adhesives after saliva contamination at different application steps[J]. Oper Dent, 2013,38(5):505-511.
doi: 10.2341/12-260-L
[13] Loguercio AD, Munoz MA, Luque-Martinez I, et al. Does active application of universal adhesives to enamel in self-etch mode improve their performance?[J]. J Dent, 2015,43(9):1060-1070.
doi: 10.1016/j.jdent.2015.04.005 pmid: 25908573
[14] Cardenas AFM, Siqueira FSF, Bandeca MC, et al. Impact of pH and application time of meta-phosphoric acid on resin-enamel and resin-dentin bonding[J]. J Mech Behav Biomed Mater, 2018,78:352-361.
doi: 10.1016/j.jmbbm.2017.11.028 pmid: 29202298
[15] Gong HH, Guo XW, Cao DF, et al. Photopolymerizable and moisture-curable polyurethanes for dental adhesive applications to increase restoration durability[J]. J Mater Chem B, 2019,7(5):744-754.
doi: 10.1039/c8tb01716f pmid: 32254848
[16] Antoniazzi BF, Nicoloso GF, Lenzi TL, et al. Selective acid etching improves the bond strength of universal adhesive to sound and demineralized enamel of primary teeth[J]. J Adhes Dent, 2016,18(4):311-316.
doi: 10.3290/j.jad.a36154 pmid: 27419240
[17] Memarpour M, Shafiei F, Razmjouei F, et al. Shear bond strength and scanning electron microscopy characteristics of universal adhesive in primary tooth dentin: An in vitro study.[J]. Dent Res J (Isfahan), 2018,15(4):264-270.
[18] Porto IM, Saiani RA, Chan KL, et al. Organic and inorganic content of fluorotic rat incisors measured by FTIR spectros-copy[J]. Spectrochim Acta A Mol Biomol Spectrosc, 2010,77(1):59-63.
doi: 10.1016/j.saa.2010.04.024 pmid: 20547096
[19] Silverstone LM, Saxton CA, Dogon IL, et al. Variation in the pattern of acid etching of human dental enamel examined by scanning electron microscopy[J]. Caries Res, 1975,9(5):373-387.
doi: 10.1159/000260179 pmid: 1055640
[20] Sumikawa DA, Marshall GW, Gee L, et al. Microstructure of primary tooth dentin[J]. Pediatr Dent, 1999,21(7):439-444.
pmid: 10633518
[21] Lenzi TL, Guglielmi Cde A, Arana-Chavez VE, et al. Tubule density and diameter in coronal dentin from primary and permanent human teeth[J]. Microsc Microanal, 2013,19(6):1445-1449.
doi: 10.1017/S1431927613012725
[22] Ye Q, Wang Y, Spencer P. Nanophase separation of polymers exposed to simulated bonding conditions[J]. J Biomed Mater Res Part B Appl Biomater, 2009,88(2):339-348.
[23] Werle SB, Steglich A, Soares FZM, et al. Effect of prolonged air drying on the bond strength of adhesive systems to dentin.[J]. Gen Dent, 2015,63(6):68-72.
pmid: 26545278
[24] Landuyt KLV, Snauwaert J, Munck JD, et al. Origin of interfacial droplets with one-step adhesives[J]. J Dent Res, 2007,86(8):739-744.
doi: 10.1177/154405910708600810 pmid: 17652202
[25] Pragasam AX, Duraisamy V, Nayak UA, et al. Evaluation of sealing ability two self-etching adhesive systems and a glass ionomer lining LC under composite restoration in primary tooth: An in vitro study[J]. J Pharm Bioallied Sci, 2015,7(Suppl 2):518-523.
doi: 10.4103/0975-7406.163525
[26] Angker L, Nockolds C, Swain MV, et al. Quantitative analysis of the mineral content of sound and carious primary dentine using BSE imaging[J]. Arch Oral Biol, 2004,49(2):99-107.
doi: 10.1016/j.archoralbio.2003.08.006
[27] van Meerbeek B, de Munck J, Yoshida Y, et al. Buonocore memorial lecture adhesion to enamel and dentin: current status and future challenges[J]. Oper Dent, 2003,28(3):215-235.
pmid: 12760693
[1] Ruo-qing ZHONG,Meng-qian ZHU,Ying-long LI,Jie PAN. Antibacterial effect of low-temperature plasma on Enterococcus faecalis in dentinal tubules in vitro [J]. Journal of Peking University (Health Sciences), 2023, 55(1): 38-43.
[2] Ruo-lan GUO,Gui-bin HUANG,Yun-zi LONG,Yan-mei DONG. Effects of novel bioactive glasses on promoting remineralization of artificial dentin caries [J]. Journal of Peking University (Health Sciences), 2023, 55(1): 82-87.
[3] Wei YONG,Kun QIAN,Wen-hao ZHU,Xiao-yi ZHAO,Chang LIU,Jie PAN. X-ray evaluation of pulp calcification in adult permanent teeth after pulpotomy [J]. Journal of Peking University (Health Sciences), 2023, 55(1): 88-93.
[4] MA Xin-rong,ZHU Xiao-ming,LI Jing,LI De-li,LI He-ping,TAN Jian-guo. Effect of a novel radio-frequency atmospheric-pressure glow discharge plasma jet treatment on crosslinking of dentin collagen [J]. Journal of Peking University (Health Sciences), 2022, 54(1): 83-88.
[5] Qiu-ju LI,Wei-yu GONG,Yan-mei DONG. Effect of bioactive glass pretreatment on the durability of dentin bonding interface [J]. Journal of Peking University (Health Sciences), 2020, 52(5): 931-937.
[6] Ming-xin MAO,Li XU,Wu-di JING,Xiao XU,Jian-xia HOU,Xiao-tong LI,Xiao-xia WANG. Alveolar crest and relevant analysis of labial side of anterior teeth on skeletal Angle class Ⅲ patients [J]. Journal of Peking University(Health Sciences), 2020, 52(1): 77-82.
[7] Yi-xiang PAN,Xiu-hua LI,Fu-cong TIAN,Xiao-yan WANG. Effect of intrapulpal pressure on the bonding strength of resin cement to dentin [J]. Journal of Peking University(Health Sciences), 2019, 51(2): 321-326.
[8] Xiao-ming ZHU,Xuan QI,De-li LI,Yu-wei ZHANG,He-ping LI,Jian-guo TAN. Effect of a novel cold atmospheric plasma jet treatment with different temperatures on resin-dentin bonding [J]. Journal of Peking University(Health Sciences), 2019, 51(1): 43-48.
[9] Wen-zhe YOU,Gui-li DOU,Bin XIA. Two-year outcomes and the influence factors of indirect pulp treatment in primary teeth: a retrospective study [J]. Journal of Peking University(Health Sciences), 2019, 51(1): 65-69.
[10] YAN Wen-juan, ZHENG Jia-jia, CHEN Xiao-Xian. Application of fluoride releasing flowable resin in pit and fissure sealant of children with early enamel caries [J]. Journal of Peking University(Health Sciences), 2018, 50(5): 911-914.
[11] LI Fang, LIU Yang, LIU Hao-chen, FENG Hai-lan . Genetic variants analysis and histological observation of teeth in a patient with hereditary opalescent dentin [J]. Journal of Peking University(Health Sciences), 2018, 50(4): 666-671.
[12] YANG Liu，CHU Xiao-yu，ZHAO Qi. Effects of RhoA on the adherens junction of murine ameloblasts [J]. Journal of Peking University(Health Sciences), 2018, 50(3): 521-526.
[13] ZANG Hai-ling, WANG Yue, LIANG Yu-hong. Cleaning efficacy of different solvents on sealer-contaminated dentin surface [J]. Journal of Peking University(Health Sciences), 2018, 50(1): 63-68.
[14] LI Hao, LIU Yu-hua, LUO Zhi-qiang. Effects of bioactive glass on reducing the hypersensitivity after full crown preparation [J]. Journal of Peking University(Health Sciences), 2017, 49(4): 709-713.
[15] XIN Yi， WANG Sai-nan， CUI Cai-yun， DONG Yan-mei. Effects of bioactive glass and extracted dentin proteins on human dental pulp cells [J]. Journal of Peking University(Health Sciences), 2017, 49(2): 331-336.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright © Journal of Peking University (Health Sciences), All Rights Reserved.
Powered by Beijing Magtech Co. Ltd