Abstract: Objective： To compare the differences of neutrophils chemotaxis ability in  peritoneal cavity between normal rats and schizopherenic rats with cell dynamic visualization system. Methods: In the study,18 healthy Kunming rats were randomly divided into 3 groups which were control group(n=6), 0.3 mg/kg MK801 treatment group(n=6）, 0.6 mg/kg dizocilpine maleate (MK-801) treatment group(n=6）, extracted neutrophils separately, and observed the morphology and counted under a microscope. Each group of cells was divided into two parts for chemotactic experiment, called chemokine agent treatment group and no chemokine agent treatment group respectively, indicating control 1,0.3 mg/kg MK801 treatment 1,0.6 mg/kg MK-801 treatment 1 and control 2,0.3 mg/kg MK-801 treatment 2,0.6 mg/kg MK-801 treatment 2.The dynamic migration of cells was recorded using the NIS-Elements software, and TAXIScan Analyzer 2 software was used to select 30 cells (n=30) in each group of cells and analyze cells migration trajectory, speed and distance, and use pair test and One-Way analysis of variance for statistical analysis. Results: The number of neutrophils in control group, 0.3 mg/kg MK-801 treatment group and 0.6 mg/kg MK-801 treatment group were（1.00±0.03）×104/mL,（0.05±0.02）×104/mL,（0.32±0.01）×104/mL respectively, the differences of results were statistically significant（P<0.05）.Under the effect of chemotactic agent,the directional migration capability of neutrophils in control group 1, 0.3 mg/kg MK-801 treatment group 1 and 0.6 mg/kg MK-801 treatment group 1 were（0.85±0.11） radian,（1.00±0.11） radian,（0.96±0.10） radian respectively （P<0.05）; the migration velocities of neutrophils were （0.09±0.02） μm/s,（0.12±0.01） μm/s,（0.14±0.01） μm/s respectively （P<0.05）;the migration distances of neutrophils were （94.26±0.02） μm,（134.61±0.01） μm,（156.19±0.01） μm respectively（P<0.05）. Conclusion: Compared with neutrophils in peritoneal cavity of control group, the neutrophils in peritoneal cavity of schizophrenic rats have stronger chemotactic movement ability.

Key words: Schizophrenia, Neutrophil, Cell chemotaxis