Journal of Peking University (Health Sciences) ›› 2022, Vol. 54 ›› Issue (2): 236-243. doi: 10.19723/j.issn.1671-167X.2022.02.006

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Effects of 275 nm and 310 nm ultraviolet irradiation on bone metabolism in ovariectomized osteoporotic rats

HE Wei1,YANG Si-wen1,CHEN Juan1,ZHU Xiao-jun2,CHEN Zhi-zhong3,MA Wen-jun1,()   

  1. 1. Department of Occupational & Environmental Health Sciences, Peking University School of Public Health, Beijing 100191, China
    2. Beijing Institute of Occupational Disease Control, Beijing 100093
    3. Condensed Matter Physics, Peking University, Beijing 100871
  • Received:2020-02-17 Online:2022-04-18 Published:2022-04-13
  • Contact: Wen-jun MA E-mail:mawenjun@bjmu.edu.cn
  • Supported by:
    National Key Research and Development Program of China(2017YFB0403104);National Natural Science Foundation of China(61674005)

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Abstract:

Objective: To investigate the effect of 275 nm and 310 nm ultraviolet irradiation on ovariectomized rats’ bone metabolism. Methods: Twenty four 3-month-old female Sprague-Dawley (SD) rat were randomly divided into control group, sham operated group, 275 nm ultraviolet (UV) irradiation group and 310 nm UV irradiation group. Each group contained 6 rats. The rats in the two irradiation groups were treated with bilateral ovariectomy. The rats in sham operated group received sham operation (They were given the same back incision and a bit of par-ovarian fat were removed). Control group received no disposition. About 24 weeks after operation, all the rats received detailed bone mineral density (BMD) detection again. Detection regions include cervical vertebra, lumbar vertebra, proximal femur, mid femur and distal femur. Next, osteopenia rats in 275 nm irradiation group were UV irradiated 275 nm with fixed illumination intensity (15 μW/cm2) everyday for 16 weeks. The osteopenia rats in 310 nm irradiation group were UV irradiated 310 nm with fixed illumination intensity (15 μW/cm 2) everyday for 16 weeks. The backs of the rats were shaved regularly as irradiation area (6 cm×8 cm). After 16-week irradiation, all the rats’ BMD of cervical vertebra, lumbar vertebra, proximal femur, mid femur and distal femur were measured. At the end of the trial, all the rats’ blood specimens were obtained and serum 25(OH)D, procollagen type Ⅰ N-peptide (PINP) and osteocalcin (OC) were measured. Results: Compared with control group [(238.78±26.74) mg/cm3], the BMD of the whole body were significantly lower in 275 nm [(193.34±13.28) mg/cm3] and 310 nm [(191.19±18.48) mg/cm3] irradiation groups (P=0.002,P=0.001). There were no significant difference between sham operated group [(227.20±14.32) mg/cm3] and control group. After 16-week ultraviolet irradiation, the BMD of the whole body were significantly increased in 275 nm [(193.34±13.28) mg/cm3 vs. (221.68±25.52) mg/cm 3,P=0.005] and 310 nm groups [(191.19±18.48) mg/cm3 vs. (267.48±20.54) mg/cm 3,P<0.001] after corresponding irradiation. The BMD of the four body regions (lumbar vertebra, proximal femur, mid femur and distal femur) had significantly increased after irradiation in 275 nm irradiation group. For 310 nm irradiation group, the BMD in cervical vertebra, lumbar vertebra, proximal femur, mid femur and distal femur also had increased significantly after 310 nm ultraviolet irradiation. The concentration of serum 25(OH)D and OC was higher in 275 nm irradiation group than in control group [(46.78±5.59) μg/L vs. (21.32±6.65) μg/L, P=0.002;(2.05±0.53) U/L vs. (1.32±0.07) U/L,P=0.022]. Compared with the control, the concentration of serum 25(OH)D [(58.05±12.74) μg/L], OC [(2.04±0.53) U/L] and PINP [(176.16±24.18) U/L] was significantly higher (P<0.001, P=0.015, P=0.005) in 310 nm irradiation group. However, there were no significantly difference between sham operated group and the control. Conclusion: Both 275 nm and 310 nm ultraviolet could improve rats’ vitamin D synthesis. Both 275 nm and 310 nm ultraviolet could improve osteopenia rats’ bone condition. The irradiation of 310 nm might be more effective on bone condition improvement.

Key words: Ultraviolet, Ovariectomized Sprague-Dawley rats, Bone metabolism, Vitamin D

CLC Number: 

  • R173.4

Figure 1

Schematic of ovariectomized osteoporotic rats modeling and ultraviolet (UV) irradiation ① refers to no disposition; ② refers to sham operation (they were given the same back incision and a bit of par-ovarian fat were removed); ③ refers to bilateral ovariectomy; ④ refers to bone mineral density (BMD) detection."

Figure 2

The sketch map of ultraviolet (UV) irradiation (A) and the sketch map of irradiation intensity detection (B); The irradiation intensity was average value calculated from five spots' measurement in cage (①-⑤)"

Figure 3

Spectrum of ultraviolet (UV) lamps used in experiment A, 275 nm UV lamp; B, 310 nm UV lamp."

Figure 4

The effect of bilateral ovariectomy on rats' bone mineral density of whole body a, P<0.05,compared with control group after operation;b, P<0.05,compared with sham operated group after operation."

Table 1

The comparison of BMD in different regions before UV irradiation ( x -±s)/(mg/cm3)"

Items BMD of whole body Cervical vertebra Lumbar vertebra Proximal femur Mid femur Distal femur
Control 238.78±26.74 190.31±24.05 251.21±75.13 351.04±31.27 365.68±28.92 345.73±36.87
Sham operated 227.20±14.32 250.06±75.01 225.27±45.15 373.56±41.64 370.31±23.55 377.89±45.75
275 nm 193.34±13.28*# 210.88±9.99 260.42±35.29 298.48±11.59*# 317.88±15.59*# 307.02±14.06*#
310 nm 191.19±18.48*# 197.69±34.42 300.98±23.66 297.32±14.62*# 302.62±25.83*# 301.33±14.04*#
F value 8.09 2.38 2.56 12.42 12.57 9.07
P value <0.001 0.099 0.083 <0.001 <0.001 <0.001

Table 2

The comparison of BMD in different regions after UV irradiation ( x -±s)/(mg/cm3)"

Items BMD of whole body Cervical vertebra Lumbar vertebra Proximal femur Mid femur Distal femur
Control 233.17±39.74 191.66±25.91 231.39±41.02 343.53±37.72 357.12±26.26 329.21±19.56
Sham operated 233.68±6.02 222.85±64.86 232.55±49.59 352.10±34.42 357.58±38.74 350.18±35.73
275 nm 221.68±25.52 224.62±48.72 289.51±27.61 323.69±45.79 358.44±48.86 353.33±44.63
310 nm 267.48±20.54 249.59±56.49 399.60±80.90*#★ 389.77±51.37 424.00±95.52 430.37±89.811★
F value 3.64 1.3 13.57 2.63 1.98 4.11
P value 0.027 0.301 <0.001 0.077 0.149 0.019

Table 3

The comparison of serum bone related biochemical indicators among different groups after ultraviolet (UV) irradiation ( x -±s)"

Items 25(OH)D/(μg/L) PINP/(U/L) OC/(U/L)
Control 21.32±6.65 129.41±2.14 1.32±0.07
Sham operated 20.42±5.76 130.12±10.17 1.32±0.08
275 nm 46.78±5.59*# 157.07±13.80 2.05±0.53*#
310 nm 58.05±12.74*# 176.16±24.18*# 2.04±0.53*#
F value 19.6 7.68 7.06
P value <0.001 0.001 0.002
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