Journal of Peking University(Health Sciences) ›› 2014, Vol. 46 ›› Issue (3): 400-404.

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Comparison of genetic damage in mice exposed to black carbon and ozone-oxidized black carbon

GAO Xin1, SHANG Jin2, YANG Jing-in1, LI Qian2, CHEN Tian1, PANG Yuan-ie1, ZHANG Wen-iao1, LUAN Xian-uo1, ZHU Tong2, JIA Guang1△   

  1. (1. Department of Occupational and Environmental Health Sciences, Peking University School of Public Health, Beijing 100191, China; 2. College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China)
  • Online:2014-06-18 Published:2014-06-18

Abstract: Objective:To make an assessment on the genotoxicity caused by black carbon (BC) and ozonized black carbon (O3-BC). Methods: In this study, 74 healthy male ICR mice [weighed (28±1.5) g] were randomly divided into 7 groups, including one phosphate buffer solution (PBS) control group and six particles exposed groups by intratracheal instillation with either BC or O3-BC at the doses of 50, 100, 200 μg/mouse, respectively. There were 12 mice in the groups of 200 μg/mouse and 10 mice in others. The mice were sacrificed 24 h after four intratrachealinstillations. The activities of catalase (CAT) in serum and the levels of malondialdehyde (MDA) in lung tissue homogenate were measured. As the DNA damage mark, 8-hydroxyguanosine (8-OHdG) in urine and serum were quantified with ELISA method. Micronucleus test was used for potential genotoxicity of BC and O3-BC. Hematoxylin and eosin staining was used to stain lung paraffin section. Results: The mice were in good condition during instillation, and the liver coefficient of the test groups was significantly lower than that of the control group (P<0.05). The activities of CAT in serum significantly increased in the 100 μg/mouse and 200 μg/mouse groups after being exposed to these two kinds of particles. The micronucleus rate in allthe BC and O3-BC exposed groups increased (P<0.05), but there was no statistically significant difference among the groups in the levels of 8-OHdG in serum and urine and MDA in lung tissue homogenate. Inflammatory response was found in the lung tissue under the microscope after exposure to BC and O3-BC. Conclusion: Intratracheal instillation of BC and O3-BC induced increasing of oxidative stress and genetic damage in mice. But there was no significant difference b

Key words: Soot, Ozone, Micronucleus tests, Mice

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