Email Alert | RSS

Journal of Peking University (Health Sciences) ›› 2021, Vol. 53 ›› Issue (2): 378-383. doi: 10.19723/j.issn.1671-167X.2021.02.024

Previous Articles     Next Articles

In vivo study of strontium-doped calcium phosphate cement for biological properties

WANG Jing-qi1,2,WANG Xiao1,Δ()   

  1. 1. Department of Stomatology, Peking University Third Hospital, Beijing 100191, China
    2. Department of Stomatology, the Second Affiliated Hospital of Hainan Medical University, Haikou 570311, China
  • Received:2019-07-09 Online:2021-04-18 Published:2021-04-21
  • Contact: Xiao WANG E-mail:bysywangxiao@163.com
  • Supported by:
    Discipline Construction Fund of Beijing Stomotology Hospital(15-09-03)

RICH HTML

 4   

PDF (PC)

139

Abstract:

Objective: To evaluate the biocompatibility and osteogenic effect of new calcium phosphate cement (CPC) in vivo and to provide experimental basis for its further clinical application. Methods: Thirty New Zealand white rabbits were randomly divided into four groups: CPC group, CPC+Bio-Oss group, Bio-Oss group and blank control group. Bone defect models of 6 mm in diameter and 7 mm in depth were made on the lateral condyle of bilateral hind legs of the rabbits. CPC, Bio-Oss and CPC+Bio-Oss mixture were implanted into the bone defect according to the group, and the mass ratio of CPC and Bio-Oss was 4 ∶1. The experimental animals were sacrificed the 4th, 12th and 24th week after operation. The tissue around the bone defect was taken for histological evaluation by H&E staining. Bone ingrowth fraction (BIF) was calculated. The expression of BMP-2 and COL-Ⅰ was detected by immunohis-tochemical staining by calculating the mean optical density (MOD) of the positive area the 4th week after operation, and the bone healing of each group was evaluated at different time points. The measurement data were analyzed by one-way ANOVA and LSD test was used for multiple comparison of the differences between the means by SPSS 19.0. P<0.05 was considered to be statistically significant. Results: The results of H&E staining showed that the BIF values of CPC group, CPC + Bio-Oss group and Bio-Oss group were significantly higher than those of blank control group at the same time point (P<0.01). The BIF values of CPC group were lower than those of Bio-Oss group and CPC + Bio-Oss group (P<0.01). There was no significant difference between CPC + Bio-Oss group and Bio-Oss group. Immunohistochemical staining showed that the MOD values of BMP-2 and COL-Ⅰ in CPC group were higher than those in blank control group, but lower than those in Bio-Oss group and CPC+Bio-Oss group (P<0.01). There was no significant difference between BMP-2 and COL-Ⅰ in CPC+Bio-Oss group and Bio-Oss group. Conclusion: The new calcium phosphate cement has good biocompatibility and can promote early osteogenesis with stable and long-term effect.

Key words: Calcium phosphate cement, Biocompatibility, Bone defect, Osteogenesis

CLC Number: 

  • R783.1

Figure 1

Histological sections at different time points of each group bone ingrowth fraction(×12.5,×200)"

Figure 2

Bone ingrowth fraction (BIF) at different time points of each group *P<0.01."

Figure 3

BMP-2 and COL-Ⅰ immunohistochemical staining of histological sections and at 4th week A, immunohistochemical staining of each group at 4th week(×40,×200); B, MOD of BMP-2 in each group; C. MOD of COL-Ⅰ in each group. *P<0.01."

Table 1

MOD of BMP-2 and col-Ⅰ immunohistochemical staining of histological sections at the 4th week ($\bar{x} \pm s$)"

Group BMP-2 COL-Ⅰ
Blank 0.496±0.016 0.121±0.026
CPC 0.134±0.021* 0.244±0.023*
Bio-Oss 0.208±0.020* 0.315±0.025*
CPC+Bio-Oss 0.197±0.022* 0.302±0.019*
[1] Choudhary S, Halbout P, Alander C, et al. Strontium ranelate promotes osteoblastic differentiation and mineralization of murine bone marrow stromal cells: involvement of prostaglandins[J]. J Bone Miner Res, 2007,22(7):1002-1010.
pmid: 17371157
[2] Baier M, Staudt P, Klein R. Strontium enhances osseointegration of calcium phosphate cement: a histomorphometric pilot study in ovariectomized rats[J]. J Orthop Surg Res, 2013,8(1):16.
[3] 李曙霞, 王京旗. 掺锶磷酸钙骨水泥材料的生物学性能体外研究[J]. 北京口腔医学, 2017,25(2):81-84.
[4] 王晓娜, 赵静辉. 骨替代材料在口腔种植领域中的成骨效果[J]. 国际口腔医学杂志, 2016,43(1):113-117.
[5] Meloni SM, Jovanovic SA, Lolli FM, et al. Grafting after sinus lift with anorganic bovine bone alone compared with 50 ∶50 anorganic bovine bone and autologous bone: results of a pilot randomised trial at one year[J]. Br J Oral Maxillofac Surg, 2015,53(5):436-441.
pmid: 25796408
[6] Aludden HC, Mordenfeld A, Hallman M, et al. Lateral ridge augmentation with Bio-Oss alone or Bio-Oss mixed with particulate autogenous bone graft: a systematic review[J]. Int J Oral Maxillofac Surg, 2017,46(8):1030-1038.
doi: 10.1016/j.ijom.2017.03.008 pmid: 28366452
[7] Wang F, Li Q, Wang Z. A comparative study of the effect of Bio-Oss in combination with concentrated growth factors or bone marrow-derived mesenchymal stem cells in canine sinus grafting[J]. J Oral Patho Med, 2017,46(7):528-536.
[8] Orsini G, Scarano A, Degidi M, et al. Histological and ultrastructural evaluation of bone around Bio-Oss particles in sinus augmentation[J]. Oral Dis, 2007,13(13):586-593.
[9] Jensen T, Schou S, Stavropoulos A, et al. Maxillary sinus floor augmentation with Bio-Oss or Bio-Oss mixed with autogenous bone as graft: a systematic review[J]. Int J Oral Maxillofac Surg, 2012,41(1):114-120.
[10] Urban IA, Nagursky H, Lozada JL, et al. Horizontal ridge augmentation with a collagen membrane and a combination of particulated autogenous bone and anorganic bovine bone-derived mineral: a prospective case series in 25 patients[J]. Int J Periodontics Restorative Dent, 2013,33(3):299-307.
pmid: 23593623
[11] Tian M, Chen F, Song W. In vivo study of porous strontium-doped calcium poly-phosphate scaffolds for bone substitute applications[J]. J Mater Sci Mater Med, 2009,20(7):1505-1512.
[12] 严广斌. 骨组织形态计量学[J]. 中华关节外科杂志: 电子版, 2016,10(2):100.
[13] 郭冲冲, 杜启翠. 细胞因子与牙槽骨重建关系的研究进展[J]. 口腔医学, 2015,35(8):697-701.
[14] Axelrad TW, Kakar S, Einhorn TA. New technologies for the enhancement of skeletal repair[J]. Injury, 2007,38(l1):49-62.
[15] 苏佳灿. 骨生长因子[M]. 第二军医大学出版社, 2015: 170-182.
[16] Marelli B, Ghezzi CE, Barralet JE, et al. Three-dimensional mineralization of dense nanofibrillar collagen bioglass hybrid scaffolds[J]. Biomacromolecules, 2010,11(6):1470-1479.
pmid: 20443577
[17] Thorwarth M, Rupprecht S, Falk S, et al. Expression of bone matrix proteins during de novo bone formation using a bovine collagen and platelet-rich plasma (prp): an immunohistochemical analysis[J]. Biomaterials, 2005,26(15):2575-2584.
pmid: 15585260
[18] 郭莉. CPC与人重组骨形成蛋白-2(rhBMP-2)复合修复即刻种植牙骨缺损的效果研究[J]. 现代仪器与医疗, 2015,6(6):104-106.
[19] Dahl SG, Allain P, Marie PJ, et al. Incorporation and distribution of strontium in bone[J]. Bone (New York), 2001,28(4):446-453.
[1] Wen-yu DU,Jing-wen YANG,Ting JIANG. Early constant observation of the effect of deferoxamine mesylate on improvement of vascularized bone regeneration in SD rat skull critical size defect model [J]. Journal of Peking University (Health Sciences), 2021, 53(6): 1171-1177.
[2] ZHANG Sheng-nan,AN Na,OUYANG Xiang-ying,LIU Ying-jun,WANG Xue-kui. Role of growth arrest-specific protein 6 in migration and osteogenic differentiation of human periodontal ligament cells [J]. Journal of Peking University (Health Sciences), 2021, 53(1): 9-15.
[3] Mei WANG, Bo-wen LI, Si-wen WANG, Yu-hua LIU. Preparation and osteogenic effect study of small intestinal submucosa sponge [J]. Journal of Peking University (Health Sciences), 2020, 52(5): 952-958.
[4] Chang CAO,Fei WANG,En-bo WANG,Yu LIU. Application of β-TCP for bone defect restore after the mandibular third molars extraction: A split-mouth clinical trial [J]. Journal of Peking University(Health Sciences), 2020, 52(1): 97-102.
[5] Ying CHEN,Zhong-ning LIU,Bo LI,Ting JIANG. Preparation of aspirin sustained-release microsphere and its in vitro releasing [J]. Journal of Peking University(Health Sciences), 2019, 51(5): 907-912.
[6] Hao WANG,Yang LIU,Hao-chen LIU,Dong HAN,Hai-lan FENG. Detection and functional analysis of BMP2 gene mutation in patients with tooth agenesis [J]. Journal of Peking University(Health Sciences), 2019, 51(1): 9-15.
[7] SUI Hua-xin, LV Pei-jun, WANG Yong, FENG Yu-chi. Effects of low level laser irradiation on the osteogenic capacity of sodium alginate/gelatin/human adipose-derived stem cells 3D bio-printing construct [J]. Journal of Peking University(Health Sciences), 2018, 50(5): 868-875.
[8] LIU Jing-yin, CHEN Fei, GE Yan-jun, WEI Ling, PAN Shao-xia, FENG Hai-lan. Influence of implants prepared by selective laser melting on early bone healing [J]. Journal of Peking University(Health Sciences), 2018, 50(1): 117-122.
[9] CHEN Fei, PAN Shao-xia, FENG Hai-lan. Distribution and content of transforming growth factor-β1 and vascular endothelial growth factor in each layer of concentrated growth factors [J]. Journal of Peking University(Health Sciences), 2016, 48(5): 860-865.
[10] QIN Xue-yan， ZHAO Hua-xiang， ZHANG Qian， CHEN Feng， LIN Jiu-xiang. NELL-1： a novel highly efficient and specific growth factor [J]. Journal of Peking University(Health Sciences), 2016, 48(2): 380-383.
[11] GE Wen-shu, TANG Yi-man, ZHANG Xiao, LIU Yun-song, ZHOU Yong-sheng. Establishing a luciferase reporter system to evaluate osteogenic differentiation potential of human adipose-derived stem cells [J]. Journal of Peking University(Health Sciences), 2016, 48(1): 170-174.
[12] SONG Yang, WANG Xiao-fei, WANG Yu-guang, SUN Yu-chun, LV Pei-jun. Osteogenesis of human adipose-derived mesenchymal stem cells-biomaterial mixture in vivo after 3D bio-printing [J]. Journal of Peking University(Health Sciences), 2016, 48(1): 45-50.
[13] 欧Meng-恩 , ZHANG Xiao, LIU Yun-Song, GE Yan-Jun, ZHOU Yong-Sheng. Ectopic osteogenesis of stromal cell-derived factor 1 combined with simvastatin loaded collagen scaffold in vivo [J]. Journal of Peking University(Health Sciences), 2015, 47(1): 47-51.
[14] DING Qian, ZHANG Feng-qiu, MA Yu-shi. Effect of icariin on osteoblastic differentiation gene expression of human periodontal ligament cells [J]. Journal of Peking University(Health Sciences), 2013, 45(6): 975-978.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] . [J]. Journal of Peking University(Health Sciences), 2009, 41(4): 456 -458 .
[2] . [J]. Journal of Peking University(Health Sciences), 2009, 41(2): 125 -128 .
[3] . [J]. Journal of Peking University(Health Sciences), 2009, 41(2): 135 -140 .
[4] . [J]. Journal of Peking University(Health Sciences), 2009, 41(2): 158 -161 .
[5] . [J]. Journal of Peking University(Health Sciences), 2009, 41(2): 217 -220 .
[6] . [J]. Journal of Peking University(Health Sciences), 2009, 41(1): 52 -55 .
[7] . [J]. Journal of Peking University(Health Sciences), 2009, 41(1): 109 -111 .
[8] . [J]. Journal of Peking University(Health Sciences), 2009, 41(3): 297 -301 .
[9] . [J]. Journal of Peking University(Health Sciences), 2009, 41(5): 505 -515 .
[10] . [J]. Journal of Peking University(Health Sciences), 2009, 41(5): 599 -601 .
Copyright © Journal of Peking University (Health Sciences), All Rights Reserved.
Powered by Beijing Magtech Co. Ltd