运动导致兴奋脑区组织液流动一过性加速

doi:10.19723/j.issn.1671-167X.2019.02.002

摘要/Abstract

摘要：

目的: 研究运动后兴奋脑区组织液(interstitial fluid, ISF)流动的变化。方法: 将20只雄性Sprague-Dawley大鼠随机分为对照组(12只)和运动组(8只), 两组大鼠均采用异氟烷进行麻醉,并动态监测尾状核区ISF流动情况,其中运动组在实验前期被放入到特制的转轮中进行运动20 min,对照组则给予持续麻醉,所有大鼠无偏瘫,运动能力良好。另外将5只大鼠采用异氟烷气体麻醉后向尾状核内植入微电极,记录运动态和麻醉态下尾状核神经元电活动。采用立体定位技术注射磁性示踪剂至尾状核ISF内,在注射前和注射后不同时间点进行一系列的磁共振扫描,直到示踪剂所致的高信号消失,采用细胞间隙定量分析系统(1.2版)对图像进行后处理和分析,监测ISF流动过程和测量相关参数,得到可反映示踪剂总量的加权信号强度(加权ΔSI)和其在ISF内的半衰期,分别计算运动组的运动前,运动后10、40、70、130和190 min各时间点以及对照组相同时间点的加权ΔSI和半衰期,采用独立样本t检验对两组测量值进行比较。结果: 微电极检测显示麻醉态和运动态下尾状核区的场电位显著不同;各时间点运动组和对照组的加权ΔSI(单位:信号强度×mm ³)分别为:运动前(60 257.1±23 069.2 vs. 61 072.0±19 547.3), 运动后10 min(83 624.3±21 475.7 vs. 71 218.1±12 586.5), 运动后40 min (57 336.0±36 243.4 vs. 69 756.1±13 306.0), 运动后70 min (43 705.9±10 246.3 vs. 55 443.2±20 733.3)、运动后130 min(7 734.9±2 645.2 vs. 8 967.6±2 007.3)和运动后190 min(2 497.3±987.5 vs. 3 013.2±1 760.8)。相对于对照组,运动组加权ΔSI在运动后40 min出现一过性的降低(P<0.05), 其余各时间点两组的加权ΔSI差异无统计学意义,两组示踪剂半衰期差异无统计学意义[(104.3±54.1) min vs. (113.4±47.3) min, P>0.05]。 结论: 运动可以导致兴奋脑区ISF流动一过性加速。

关键词: 脑, 磁共振成像, 组织液, 示踪剂, 大鼠

Abstract:

Objective: To investigate the changes of brain interstitial fluid (ISF) induced by movement.Methods: Twenty mature male Sprague-Dawley rats were randomly divided into two groups: control group and movement group. Electrophysiological neurons in caudate nuclear of additional five rats were recorded and the differences analyzed between under anesthesia and by movement. In the control group, the rats were anesthetized using isoflurane continuously during the experiment process. In the meantime the magnetic tracer was injected into the center of the caudate nucleus and multi-period magne-tic resonance scanning was performed at several time points until high signal intensity invisible in the images. In the movement group, the rats were anesthetized for the injection of the tracer, and the first post-injection magnetic resonance scanning was performed. Then the rats were waken and allowed moving voluntarily for 20 minutes. The rats were anesthetized again and multi-period magnetic resonance scanning was performed until the experiment ended. NanoDetect system (Version 1.2, MRI lab, Beijing, China) was used to measure the parameters on ISF, which included the weighed signal intensity (weighed ΔSI) , the term predicting the amount of the tracer, and half-time of the tracer. In movement group, the weighed ΔSI at the time points of pre-movement and 10, 40, 70, 130, and 190 minutes after movement were calculated respectively. In control group, the weighed ΔSI at the same time points also were measured. The weighed ΔSI and half-time were compared between the two groups.Results: The electrophysiological recording and data analysis showed significant difference in the local field potential of Caudate Nucleus between under anesthesia and by movement. The weighed ΔSI (unit: ΔSI×mm ³) va-lues of the two groups, presented by movement group vs. control group, were as followings, 60 257.1±23 069.2 vs. 61 072.0±19 547.3 at pre-move, 83 624.3±21 475.7 vs. 71 218.1±12 586.5 at 10 min after movement, 57 336.0±36 243.4 vs. 69 756.1±13 306.0 at 40 min after movement, 43 705.9±10 246.3 vs. 55 443.2±20 733.3 at 70 min after movement, 7 734.9±2 645.2 vs. 8 967.6±2 007.3 at 130 min after movement and 2 497.3±987.5 vs. 3 013.2±1 760.8 at 190 min after movement. Moreover, at 40 min after movement, the weighed ΔSI of movement group was significantly reduced compared with control group (P<0.05). The half-time was not significantly different [(104.3±54.1) min vs. (113.4±47.3) min, P>0.05]. Conclusion: ISF drainage of caudate nuclear can be acclerated temporarily by movement.

Key words: Brain, Magnetic resonance imaging, Interstitial fluid, Tracer, Rats

中图分类号:

R445

王伟,侯进,黄文强. 运动导致兴奋脑区组织液流动一过性加速[J]. 北京大学学报（医学版）, 2019, 51(2): 206-209.

Wei WANG,Jin HOU,Wen-qiang HUANG. Temporary acceleration of interstitial fluid drainage in excited brain region induced by movement[J]. Journal of Peking University(Health Sciences), 2019, 51(2): 206-209.

图/表 2

图1

图2

参考文献 19

[1]	He C, Chen F, Li B , et al. Neurophysiology of HCN channels: from cellular functions to multiple regulations[J]. Prog Neuro-biol, 2014,112(1):1-23. doi: 10.1016/j.pneurobio.2013.10.001
[2]	Frischknecht R, Gundelfinger ED . The brain’s extracellular matrix and its role in synaptic plasticity[J]. Adv Exp Med Biol, 2012,970(1):153-171. doi: 10.1007/978-3-7091-0932-8
[3]	Deco G, Rolls ET, Albantakis L , et al. Brain mechanisms for perceptual and reward-related decision-making[J]. Prog Neuro-biol, 2013,103(4):194-213. doi: 10.1016/j.pneurobio.2012.01.010
[4]	杨双风, 韩鸿宾, 彭芸 . 大鼠生后发育过程中脑细胞外间隙的解剖及生理特性的变化[J]. 中国比较医学杂志, 2015,25(3):73-79.
[5]	Shi C, Lei Y, Han H , et al. Transportation inthe interstitial space of the brain can be regulated by neuronal excitation[J]. Sci Rep, 2015,5(12):17673.
[6]	Liu B, Bai X, Zhou F , et al. Mutual information based three-dimensional registration of rat brain magnetic resonance imaging time-series[J]. Comput Electr Eng, 2013,39(5):1473-1484. doi: 10.1016/j.compeleceng.2012.11.026
[7]	Han H, Shi C, Fu Y , et al. A novel MRI tracer-based method for measuring waterdiffusion in the extracellular space of the rat brain[J]. IEEE J Biomed Health Inform, 2014,18(3):978-983. doi: 10.1109/JBHI.2014.2308279
[8]	Lei Y, Han H, Yuan F , et al. The brain interstitial system: Ana-tomy, modeling, in vivo measurement, and applications[J]. Prog Neurobiol, 2017,157(10):230-246. doi: 10.1016/j.pneurobio.2015.12.007
[9]	刘娥, 张艺璇, 孙琳琳 , 等. 水通道蛋白4在阿尔兹海默病脑组织间液引流改变中的作用[J]. 北京大学学报(医学版), 2018,50(2):203-206.
[10]	Xie L, Kang H, Xu Q , et al. Sleep drives metabolite clearance from the adult brain[J]. Science, 2013,342(6156):373-377. doi: 10.1126/science.1241224
[11]	李学义, 王伟, 韩鸿宾 , 等. 采用磁共振示踪法探讨大鼠脑细胞间隙内物质转运清除规律[J]. 中国医学影像技术, 2018,34(1):1-4.
[12]	Lv D, Li J, Li H , et al. Imaging and quantitative analysis of the interstitial space in the caudate nucleus in a rotenone-induced rat model of Parkinson’s disease using tracer-based MRI[J]. Aging Dis, 2017,8(1):1-6. doi: 10.14336/AD.2016.0625
[13]	Watanabe M, Munoz DP . Saccade suppression by electrical microstimulation in monkey caudate nucleus[J]. J Neurosci, 2010,30(7):2700-2709. doi: 10.1523/JNEUROSCI.5011-09.2010
[14]	Kravitz AV, Kreitzer AC . Striatal mechanisms underlying movement, reinforcement, and punishment[J]. Physiology, 2012,27(3):167-177. doi: 10.1152/physiol.00004.2012
[15]	Hikosaka O, Kim HF, Yasuda M , et al. Basal ganglia circuits for reward value-guided behavior[J]. Annu Rev Neurosci, 2014,37(8):289-306. doi: 10.1146/annurev-neuro-071013-013924
[16]	Lee SH, Koh JS, Ryu CW , et al. Changes of motor deactivation regions in patients with intracranial lesions[J]. J Korean Neurosurg Soc, 2013,54(6):453-460. doi: 10.3340/jkns.2013.54.6.453
[17]	Hughes LE, Altena E, Barker RA , et al. Perseveration and choice in Parkinson’s disease: the impact of progressive frontostriatal dysfunction on action decisions[J]. Cerebral Cortex, 2013,23(7):1572-1581. doi: 10.1093/cercor/bhs144
[18]	Hou J, Wang W, Quan X , et al. Quantitativevisualization of dynamic tracer transportation in the extracellular space of deep brain regions using tracer-based magnetic resonance imaging[J]. Med Sci Monit, 2017,23(9):4260-4268. doi: 10.12659/MSM.903010
[19]	滕泽, 王伟, 关湘萍 , 等. 大脑类淋巴系统的研究进展[J]. 中华老年心脑血管病杂志, 2017,19(9):1001-1003.

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