Email Alert | RSS

北京大学学报（医学版） ›› 2024, Vol. 56 ›› Issue (4): 687-692. doi: 10.19723/j.issn.1671-167X.2024.04.023

• 论著 • 上一篇    下一篇

亚慢性PM2.5和O3共同暴露对大鼠鼻黏膜ATP总量及ATP酶活性的影响

阎腾龙1,2,胥嘉钰1,陈田3,杨鑫3,王伟伟4,周淑佩5,牛丕业3,贾光1,夏交4,*()   

  1. 1. 北京大学公共卫生学院劳动卫生与环境卫生学系，北京 100191
    2. 北京市职业病防治研究院，北京 100093
    3. 首都医科大学公共卫生学院，首都医科大学环境毒理学北京市重点实验室，北京 100069
    4. 首都医科大学附属北京友谊医院耳鼻咽喉头颈外科，北京 100050
    5. 北京大学医学部实验动物科学部，北京 100191
  • 收稿日期:2021-01-26 出版日期:2024-08-18 发布日期:2024-07-23
  • 通讯作者: 夏交 E-mail:jiaoxia0322@msn.com
  • 基金资助:
    国家自然科学基金(91643111);国家自然科学基金(91743114)

Effects of PM2.5 and O3 sub-chronic combined exposure on ATP amount and ATPase activities in rat nasal mucosa

Tenglong YAN1,2,Jiayu XU1,Tian CHEN3,Xin YANG3,Weiwei WANG4,Shupei ZHOU5,Piye NIU3,Guang JIA1,Jiao XIA4,*()   

  1. 1. Department of Occupational and Environmental Health Sciences, Peking University School of Public Health, Beijing 100191, China
    2. Beijing Institute of Occupational Disease Prevention and Treatment, Beijing 100093, China
    3. School of Public Health and the Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China
    4. Department of Otorhinolaryngology, Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
    5. Department of Laboratory Animal Science, Peking University Health Science Center, Beijing 100191, China
  • Received:2021-01-26 Online:2024-08-18 Published:2024-07-23
  • Contact: Jiao XIA E-mail:jiaoxia0322@msn.com
  • Supported by:
    the National Natural Science Foundation of China(91643111);the National Natural Science Foundation of China(91743114)

RICH HTML

   

PDF (PC)

摘要：

目的: 探讨细颗粒物(fine particle matter，PM2.5)和臭氧(ozone，O3)亚慢性共同暴露对大鼠鼻黏膜组织腺嘌呤核苷三磷酸(adenosine triphosphate, ATP)总量及ATP酶活性的影响。方法: 采用随机数字表法将20只雄性Sprague Dawley (SD) 大鼠均分为对照组和暴露组，每组各10只，分别饲养于常规清洁级环境和本团队既往所搭建的大气污染物暴露系统中，连续暴露208 d。暴露期间，监测暴露系统内PM2.5和O3浓度，采用自测和站点数据相结合的方法对暴露系统内PM2.5和O3进行综合评估。在暴露第208天处死大鼠取心、肝、脾、肾、睾丸等主要器官和鼻黏膜组织，称量各脏器重量并计算脏器系数。利用所收集鼻黏膜组织，使用生物发光法测定ATP总量，使用分光光度法检测Na+-K+-ATP酶和Ca2+-ATP酶活性。使用两独立样本t检验比较各指标组间差异。结果: 自第3周开始至暴露结束，暴露组大鼠体质量高于对照组(P < 0.05)，两组间脏器系数差异无统计学意义。暴露组日均PM2.5浓度为(30.68 ± 19.23) μg/m3，O3最大8 h浓度(O3-8 h)为(82.45 ± 35.81) μg/m3。暴露组大鼠鼻黏膜组织ATP化学发光值(792.4±274.1) IU/L低于对照组(1 126.8±218.1) IU/L，鼻黏膜组织Na+-K+-ATP酶活性(1.53±0.85) U/mg低于对照组(4.31±1.60) U/mg(P < 0.05)。对照组和暴露组鼻黏膜组织的蛋白含量分别为(302.14±52.51) mg/L和(234.58±53.49) mg/L，Ca2+-ATP酶活性分别为(0.81±0.27) U/mg和(0.99± 0.73) U/mg，组间差异均无统计学意义。结论: 亚慢性PM2.5和O3共同暴露可能影响大鼠鼻黏膜组织供氧能力。

关键词: 细颗粒物, 臭氧, 腺嘌呤核苷三磷酸

Abstract:

Objective: To evaluate the effects of fine particle matter (PM2.5) and ozone (O3) combined exposure on adenosine triphosphate (ATP) amount and ATPase activities in nasal mucosa of Sprague Dawley (SD) rats. Methods: Twenty male SD rats were divided into control group (n=10) and exposure group (n=10) by random number table method. The rats were fed in the conventional clean environment and the air pollutant exposure system established by our team, respectively, and exposed for 208 d. During the exposure period, the concentrations of PM2.5 and O3 in the exposure system were monitored, and a comprehensive assessment of PM2.5 and O3 in the exposure system was conducted by combining self-measurement and site data. On the 208 d of exposure, the core, liver, spleen, kidney, testis and other major organs and nasal mucosal tissues of the rats were harvested. Each organ was weighed and the organ coefficient calculated. The total amount of ATP was measured by bioluminescence, and the activities of Na+-K+ -ATPase and Ca2+ -ATPase were detected by spectrophotometry. The t test of two independent samples was used to compare the differences among the indicator groups. Results: From the 3rd week to the end of exposure duration, the body weight of the rats in the exposure group was higher than that in the control group (P < 0.05), and there was no significant difference in organ coefficients between the two groups. The average daily PM2.5 concentration in the exposure group was (30.68±19.23) μg/m3, and the maximum 8 h ozone concentration (O3-8 h) was (82.45±35.81) μg/m3. The chemiluminescence value (792.4±274.1) IU/L of ATP in nasal mucosa of the rats in the exposure group was lower than that in the control group (1 126.8±218.1) IU/L. The Na+-K+-ATPase activity (1.53±0.85) U/mg in nasal mucosa of the rats in the exposure group was lower than that in the control group (4.31±1.60) U/mg (P < 0.05). The protein content of nasal mucosa in the control group and the exposure group were (302.14±52.51) mg/L and (234.58±53.49) mg/L, respectively, and the activity of Ca2+-ATPase was (0.81±0.27) U/mg and (0.99±0.73) U/mg, respectively. There was no significant difference between the groups. Conclusion: The ability of power capacity decreased in the rat nasal mucossa under the sub-chronic low-concentration exposure of PM2.5 and O3.

Key words: Fine particle matter, Ozone, Adenosine triphosphate

中图分类号: 

  • R122.7

图1

暴露期间动物体质量监测及一般毒性评价"

图2

暴露系统内和监测站PM2.5浓度"

图3

暴露系统内和监测站点O3-8 h浓度"

1 World Health Organization. Concentration of fine particle matter (PM2.5)[EB/OL]. (2019-08-28)[2020-08-12]. https://www.who.int/data/gho/data/indicators/indicator-details/GHO/concentrations-of-fine-particulate-matter-(pm2-5).
2 中华人民共和国生态环境部. 2015年中国生态环境状况公报[R/OL]. (2016-06-02)[2020-10-08]. https://www.mee.gov.cn/ywdt/tpxw/201606/t20160602_353077.shtml.
3 中华人民共和国生态环境部. 2016年中国生态环境状况公报[R/OL]. (2017-06-05)[2020-11-02]. https://www.mee.gov.cn/gkml/sthjbgw/qt/201706/t20170605_415442.htm.
4 中华人民共和国生态环境部. 2017年中国生态环境状况公报[R/OL]. (2018-05-31)[2020-10-05]. https://www.mee.gov.cn/hjzl/sthjzk/zghjzkgb/201805/P020180531534645032372. pdf.
5 中华人民共和国生态环境部. 2018年中国生态环境状况公报[R]. (2019-05-29)[2020-11-07]. https://www.mee.gov.cn/ywdt/tpxw/201905/t20190529_704841.shtml.
6 孙宏发. 基于CT图像的真实成人上呼吸道内气流特性及颗粒物沉积研究[D]. 陕西: 西安建筑科技大学, 2015.
7 Sundukov YN . First record of the ground beetle Trechoblemus postilenatus (Coleoptera, Carabidae) in Primorskii krai[J]. Far Eastern Entomologist, 2006, 165 (4): 16.
8 World Health Organization. Exposure to ambient air pollution from particulate matter for 2016[R/OL]. (2018-04-02)[2020-10-10]. https://cdn.who.int/media/docs/default-source/air-quality-database/aqd-2018/aap_exposure_apr2018_final.pdf?sfvrsn=86d2fad9_3.
9 Cabrera M , Garzón García B , Moreno-Grau S , et al. Association between seasonal allergic rhinitis and air pollution, meteorological factors, and grass pollen counts in madrid (1996 and 2009)[J]. J Investig Allergol Clin Immunol, 2019, 29 (5): 371- 377.
doi: 10.18176/jiaci.0368
10 Brant T , Brant T , Yoshida C , et al. Mucociliary clearance, airway inflammation and nasal symptoms in urban motorcyclists[J]. Clinics, 2014, 69 (12): 867- 870.
doi: 10.6061/clinics/2014(12)13
11 Jia JX , Xia J , Zhang RX , et al. Investigation of the impact of PM2.5 on the ciliary motion of human nasal epithelial cells[J]. Chemosphere, 2019, 233 (5): 309- 318.
12 Li FX , Ma HH , Liu J . Pyrethroid insecticide cypermethrin modulates gonadotropin synthesis via calcium homeostasis and ERK1/2 signaling in LβT2 mouse pituitary cells[J]. Toxicol Sci, 2018, 162 (1): 43- 52.
doi: 10.1093/toxsci/kfx248
13 Dizier MH , Margaritte-Jeannin P , Pain L , et al. Interactive effect between ATPase-related genes and early-life tobacco smoke exposure on bronchial hyper-responsiveness detected in asthma-ascertained families[J]. Thorax, 2019, 74 (3): 254- 260.
doi: 10.1136/thoraxjnl-2018-211797
14 阎腾龙, 夏交, 胥嘉钰, 等. 北京大气污染物暴露对大鼠嗅觉的影响[J]. 中华预防医学杂志, 2020, 54 (7): 774- 778.
doi: 10.3760/cma.j.cn112150-20200508-00699
15 Ostrowski LE , Bennett WD . Cilia and mucociliary clearance[J]. Encycl Respir Med, 2006, 10 (1101): 466- 470.
16 Angelis N , Spyratos D , Domvri K , et al. Effect of ambient ozone exposure assessed by individual monitors on nasal function and exhaled NO among school children in the area of Thessaloniki, Greece[J]. J Occup Environ Med, 2017, 59 (6): 509- 515.
doi: 10.1097/JOM.0000000000001011
17 宋晓玲, 邢志敏, 余力生. 大气可吸入颗粒物对变应性鼻炎大鼠鼻黏膜的影响[J]. 中国耳鼻咽喉头颈外科, 2008, 15 (4): 223- 226.
18 Wagner JG , Hotchkiss JA , Harkema JR . Enhancement of nasal inflammatory and epithelial responses after ozone and allergen coexposure in Brown Norway rats[J]. Toxicol Sci, 2002, 67 (2): 284- 294.
doi: 10.1093/toxsci/67.2.284
19 Gudis D , Zhao KQ , Cohen NA . Acquired cilia dysfunction in chronic rhinosinusitis[J]. Am J Rhinol Allergy, 2012, 26 (1): 1- 6.
doi: 10.2500/ajra.2012.26.3716
20 Andreoli SM , Schlosser RJ , Wang LF , et al. Adenoid ciliostimulation in children with chronic otitis media[J]. Otolaryngol Head Neck Surg, 2013, 148 (1): 135- 139.
doi: 10.1177/0194599812462664
21 Guo ZQ , Hong ZC , Dong WY , et al. PM2.5-induced oxidative stress and mitochondrial damage in the nasal mucosa of rats[J]. Int J Environ Res Public Health, 2017, 14 (2): 134.
doi: 10.3390/ijerph14020134
22 中华人民共和国生态环境部. 环境空气质量标准[EB/OL]. (2016-01-01)[2020-10-11]. https://www.mee.gov.cn/ywgz/fgbz/bz/bzwb/dqhjbh/dqhjzlbz/201203/t20120302_224165.shtml.
23 Zhang ZL , Dong B , Chen GB , et al. Ambient air pollution and obesity in school-aged children and adolescents: A multicenter study in China[J]. Sci Total Environ, 2021, 771, 144583.
doi: 10.1016/j.scitotenv.2020.144583
24 Jin XT , Su H , Ding G , et al. Exposure to ambient fine particles causes abnormal energy metabolism and ATP decrease in lung tissues[J]. Chemosphere, 2019, 224, 29- 38.
doi: 10.1016/j.chemosphere.2019.02.116
25 de Sá MC , Nakagawa NK , de André CDS , et al. Aerobic exercise in polluted urban environments: Effects on airway defense mechanisms in young healthy amateur runners[J]. J Breath Res, 2016, 10 (4): 1- 9.
26 Skou JC , Esmann M . The Na+-K+-ATPase[J]. J Bioenerg Biomembr, 1992, 24 (3): 249- 261.
27 Anbarasi K , Vani G , Balakrishna K , et al. Effect of bacoside A on membrane-bound ATPases in the brain of rats exposed to cigarette smoke[J]. J Biochem Mol Toxic, 2005, 19 (1): 59- 65.
28 Demir TA , Akar T , Akyüz F , et al. Nickel and cadmium concentrations in plasma and Na+/ K+ATPase activities in erythrocyte membranes of the people exposed to cement dust emissions[J]. Environ Monit Assess, 2005, 104 (1/2/3): 437- 444.
29 Li RJ , Kou XJ , Geng H , et al. Effect of ambient PM2.5 on lung mitochondrial damage and fusion/fission gene expression in rats[J]. Chem Res Toxicol, 2015, 28 (3): 408- 418.
30 Giraud F , Claret M , Garay R . Interactions of cholesterol with the Na pump in red blood cells[J]. Nature, 1976, 264 (5587): 646- 648.
31 Duchnowicz P , Koter M . Damage to the erythrocyte membrane caused by chlorophenoxyacetic herbicides[J]. Cel Mol Biol Lett, 2003, 8 (1): 25- 30.
[1] 张宏,董继元,王建军,范临夏,曲强,刘洋. 兰州市臭氧对儿童哮喘的短期影响及其季节性差异[J]. 北京大学学报（医学版）, 2022, 54(2): 227-235.
[2] 白枫,何倚帆,牛亚楠,杨若娟,曹静. 超细颗粒物对大鼠离体灌注心脏功能的影响[J]. 北京大学学报（医学版）, 2021, 53(2): 240-245.
[3] 陈嘉惠,胡大宇,贾旭,牛薇,邓芙蓉,郭新彪. 大气臭氧短期监测指标与健康年轻人肺功能和气道炎症的关联[J]. 北京大学学报（医学版）, 2020, 52(3): 492-499.
[4] 潘小川. 关注中国大气灰霾（PM2.5）对人群健康影响的新常态[J]. 北京大学学报（医学版）, 2015, 47(3): 377-379.
[5] 郜鑫, 尚静, 杨敬林, 李前, 陈田, 庞元捷, 张文晓, 栾先国, 朱彤, 贾光. 黑碳和臭氧处理的黑碳对小鼠遗传损伤的比较研究[J]. 北京大学学报（医学版）, 2014, 46(3): 400-404.
[6] 王欣, 邓芙蓉, 吴少伟, 郑迎东, 孙秀明, 刘红, 郭新彪. 北京市某区大气可吸入颗粒物和细颗粒物对儿童肺功能的短期影响[J]. 北京大学学报（医学版）, 2010, 42(3): 340-344.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
ISSN 1671-167X
CN 11-4691/R
主管单位：中华人民共和国教育部
主办单位：北京大学
地址： 北京市海淀区学院路38号 北京大学医学部 《北京大学学报（医学版）》
邮编：100191
电话：010-82801551
Email： xbbjb2@bjmu.edu.cn

《北京大学学报（医学版）》
微信公众号
版权所有 © 2018 《北京大学学报（医学版）》编辑部